Aspergillus ellipticus CBS 707.79
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12861 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A319D1A5|A0A319D1A5_9EURO Uncharacterized protein OS=Aspergillus ellipticus CBS 707.79 OX=1448320 GN=BO71DRAFT_67798 PE=4 SV=1
MM1 pKa = 8.03 GDD3 pKa = 3.49 TFHH6 pKa = 7.89 PDD8 pKa = 3.22 NSWSLLDD15 pKa = 5.4 DD16 pKa = 4.08 DD17 pKa = 4.71 TPAYY21 pKa = 10.43 DD22 pKa = 5.15 DD23 pKa = 3.99 YY24 pKa = 11.88 PEE26 pKa = 4.73 VIPLATTEE34 pKa = 4.17 YY35 pKa = 10.63 VDD37 pKa = 4.1 PFPAPFSTQGYY48 pKa = 8.69 APRR51 pKa = 11.84 EE52 pKa = 4.0 IIQAVWSVSGAPSNQNIPAAAPVPNTNPDD81 pKa = 3.37 PAAAPVIDD89 pKa = 4.48 PNLNQTAAPTVDD101 pKa = 3.59 SNLNNTEE108 pKa = 4.02 APVVDD113 pKa = 4.04 PGLYY117 pKa = 9.92 PSVAPGTTFYY127 pKa = 10.79 FDD129 pKa = 3.23 QVVGVFTGSDD139 pKa = 3.98 LDD141 pKa = 3.56 PTMPLFLEE149 pKa = 4.88 PDD151 pKa = 2.98 WSFPPLIQSMPPGVSPTADD170 pKa = 3.45 ATPTAAVDD178 pKa = 3.51 ATMDD182 pKa = 4.0 PAIDD186 pKa = 4.1 PSQDD190 pKa = 3.03 VAADD194 pKa = 3.67 PAIDD198 pKa = 4.11 PSQDD202 pKa = 3.03 VAADD206 pKa = 3.64 PAIDD210 pKa = 4.26 PNLDD214 pKa = 3.03 AAADD218 pKa = 4.0 PNIDD222 pKa = 3.91 PNLDD226 pKa = 3.12 AAADD230 pKa = 4.08 PNIHH234 pKa = 6.89 SDD236 pKa = 3.99 LDD238 pKa = 4.16 LAALWAEE245 pKa = 4.67 LDD247 pKa = 3.43 QTAAPGNDD255 pKa = 3.05 TGPAQTVVPAVNPHH269 pKa = 6.07 LQLTLDD275 pKa = 4.35 DD276 pKa = 4.3 FQIPEE281 pKa = 4.49 PDD283 pKa = 3.35 QPLLINSFDD292 pKa = 3.89 LVIHH296 pKa = 6.21 GFEE299 pKa = 4.29 DD300 pKa = 3.41 VPYY303 pKa = 9.27 ILPPEE308 pKa = 4.35 PPGPQIVMVQKK319 pKa = 10.65 RR320 pKa = 11.84 YY321 pKa = 8.05 QCPWYY326 pKa = 10.18 GCRR329 pKa = 11.84 TILRR333 pKa = 11.84 TLVGLRR339 pKa = 11.84 AHH341 pKa = 6.8 IRR343 pKa = 11.84 LEE345 pKa = 4.05 HH346 pKa = 6.03 QGRR349 pKa = 11.84 PTVRR353 pKa = 11.84 CPYY356 pKa = 10.33 CPLKK360 pKa = 10.98 YY361 pKa = 10.46 GEE363 pKa = 4.95 GYY365 pKa = 10.09 NLHH368 pKa = 6.41 RR369 pKa = 11.84 HH370 pKa = 5.19 VRR372 pKa = 11.84 WKK374 pKa = 10.88 HH375 pKa = 5.58 PDD377 pKa = 3.37 FLDD380 pKa = 4.28 DD381 pKa = 4.56 FVNFAPFF388 pKa = 3.3
Molecular weight: 41.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.757
IPC_protein 3.808
Toseland 3.567
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.617
Grimsley 3.465
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.24
Thurlkill 3.63
EMBOSS 3.783
Sillero 3.935
Patrickios 1.049
IPC_peptide 3.808
IPC2_peptide 3.897
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A319DGA6|A0A319DGA6_9EURO NIF-domain-containing protein OS=Aspergillus ellipticus CBS 707.79 OX=1448320 GN=BO71DRAFT_320935 PE=4 SV=1
MM1 pKa = 7.51 SSSSQNPSPKK11 pKa = 10.32 GKK13 pKa = 9.83 NPKK16 pKa = 9.16 NQPQTPTPTSKK27 pKa = 10.89 SNPLRR32 pKa = 11.84 ARR34 pKa = 11.84 ARR36 pKa = 11.84 ARR38 pKa = 11.84 ARR40 pKa = 11.84 ARR42 pKa = 11.84 ARR44 pKa = 11.84 VRR46 pKa = 11.84 VRR48 pKa = 11.84 ARR50 pKa = 11.84 VRR52 pKa = 11.84 VRR54 pKa = 11.84 RR55 pKa = 11.84 ARR57 pKa = 11.84 ARR59 pKa = 11.84 PLRR62 pKa = 11.84 NNPNNPNNPNPTPNQHH78 pKa = 6.16 ATPPSPLAAFPRR90 pKa = 11.84 SVTCAGAA97 pKa = 3.3
Molecular weight: 10.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.499
IPC2_protein 11.111
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.364
Rodwell 12.471
Grimsley 12.925
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.193
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12861
0
12861
5629130
49
6272
437.7
48.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.507 ± 0.018
1.337 ± 0.008
5.549 ± 0.017
5.956 ± 0.021
3.768 ± 0.013
6.915 ± 0.021
2.49 ± 0.009
4.923 ± 0.016
4.286 ± 0.021
9.197 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.188 ± 0.008
3.493 ± 0.012
6.232 ± 0.026
3.981 ± 0.015
6.219 ± 0.018
8.327 ± 0.025
6.042 ± 0.016
6.229 ± 0.017
1.514 ± 0.009
2.847 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here