Buchnera aphidicola (Cinara tujafilina)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola

Average proteome isoelectric point is 8.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 359 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7WZ75|F7WZ75_9GAMM ATP-binding cell division protein OS=Buchnera aphidicola (Cinara tujafilina) OX=261317 GN=ftsA PE=4 SV=1
MM1 pKa = 7.52FEE3 pKa = 4.43PVNLEE8 pKa = 3.17NDD10 pKa = 4.22AIIKK14 pKa = 9.74VIGIGGGGSNAVEE27 pKa = 3.65HH28 pKa = 6.7MIRR31 pKa = 11.84EE32 pKa = 4.32KK33 pKa = 10.73IEE35 pKa = 3.84GVEE38 pKa = 4.14FFAINTDD45 pKa = 3.23AQALRR50 pKa = 11.84KK51 pKa = 9.14IDD53 pKa = 3.51VGQTIQIGNNITKK66 pKa = 10.6GLGAGANPEE75 pKa = 4.15VGRR78 pKa = 11.84TAAEE82 pKa = 3.86EE83 pKa = 3.79DD84 pKa = 3.8TEE86 pKa = 4.22RR87 pKa = 11.84LKK89 pKa = 11.19SALEE93 pKa = 4.07GADD96 pKa = 3.66MIFIAAGMGGGTGTGATPIIAKK118 pKa = 9.68IARR121 pKa = 11.84DD122 pKa = 3.73LGILTVAVVTKK133 pKa = 10.06PFSFEE138 pKa = 3.59GKK140 pKa = 9.81KK141 pKa = 10.53RR142 pKa = 11.84MIYY145 pKa = 10.4AEE147 pKa = 4.05QGLQEE152 pKa = 4.27LSKK155 pKa = 11.2SVDD158 pKa = 3.45SLITIPNDD166 pKa = 3.11KK167 pKa = 10.19LLKK170 pKa = 9.37VLSRR174 pKa = 11.84GISLLDD180 pKa = 3.39AFKK183 pKa = 10.75AANDD187 pKa = 3.61ILKK190 pKa = 10.68GAVQGIAEE198 pKa = 5.59LITRR202 pKa = 11.84PGLMNVDD209 pKa = 4.24FADD212 pKa = 3.24VRR214 pKa = 11.84TVMSEE219 pKa = 3.25MGYY222 pKa = 11.54AMMGTGSASGEE233 pKa = 3.9NRR235 pKa = 11.84AEE237 pKa = 4.07EE238 pKa = 4.11ASEE241 pKa = 3.91IAISSPLLEE250 pKa = 6.07DD251 pKa = 3.85IDD253 pKa = 4.68LSGTKK258 pKa = 10.14GVLVNITAGFDD269 pKa = 3.28LRR271 pKa = 11.84LDD273 pKa = 3.57EE274 pKa = 5.7FEE276 pKa = 4.61TVGNTIRR283 pKa = 11.84AFSSDD288 pKa = 3.06NATVVIGTSLDD299 pKa = 3.48PKK301 pKa = 9.77MEE303 pKa = 3.95EE304 pKa = 4.05SLRR307 pKa = 11.84VTVVATGIGGDD318 pKa = 3.37KK319 pKa = 10.74HH320 pKa = 8.19SDD322 pKa = 2.72IMLMNNRR329 pKa = 11.84SSKK332 pKa = 11.37DD333 pKa = 3.22MLLGYY338 pKa = 8.52QNKK341 pKa = 10.1LSLNQNDD348 pKa = 4.01TNNTYY353 pKa = 10.65SEE355 pKa = 4.15NKK357 pKa = 9.18MKK359 pKa = 10.54EE360 pKa = 4.04KK361 pKa = 10.79NITPNNQNEE370 pKa = 4.51KK371 pKa = 10.45IFF373 pKa = 3.86

Molecular weight: 39.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7WYV9|F7WYV9_9GAMM 10 kDa chaperonin OS=Buchnera aphidicola (Cinara tujafilina) OX=261317 GN=groS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.24IKK11 pKa = 10.37RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 9.67NGRR28 pKa = 11.84HH29 pKa = 4.79ILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84IKK37 pKa = 10.76SRR39 pKa = 11.84IRR41 pKa = 11.84LCVV44 pKa = 2.96

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

359

0

359

115088

38

1420

320.6

36.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.909 ± 0.107

1.372 ± 0.048

3.917 ± 0.086

4.305 ± 0.125

5.3 ± 0.152

5.182 ± 0.123

2.178 ± 0.044

12.742 ± 0.163

10.82 ± 0.199

10.052 ± 0.113

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.045

7.622 ± 0.131

2.964 ± 0.054

3.244 ± 0.053

3.559 ± 0.096

6.666 ± 0.085

4.546 ± 0.07

4.333 ± 0.094

0.852 ± 0.044

4.311 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski