Wolfiporia cocos (strain MD-104) (Brown rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Laetiporaceae; Wolfiporia; Wolfiporia cocos

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12197 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H3JMR7|A0A2H3JMR7_WOLCO Uncharacterized protein OS=Wolfiporia cocos (strain MD-104) OX=742152 GN=WOLCODRAFT_18207 PE=4 SV=1
MM1 pKa = 8.07IFEE4 pKa = 5.67DD5 pKa = 4.08PEE7 pKa = 4.45QYY9 pKa = 11.17NSTDD13 pKa = 3.53FKK15 pKa = 11.48VILDD19 pKa = 3.88PAHH22 pKa = 5.28VQEE25 pKa = 5.01VRR27 pKa = 11.84DD28 pKa = 4.38LYY30 pKa = 11.31ALPTDD35 pKa = 3.9PVFQLVPPAFRR46 pKa = 11.84DD47 pKa = 4.11LADD50 pKa = 3.82LLYY53 pKa = 9.8WQMGHH58 pKa = 7.11PLVIYY63 pKa = 7.99LTAWAIYY70 pKa = 10.49ADD72 pKa = 4.09LLNRR76 pKa = 11.84IRR78 pKa = 11.84TAGDD82 pKa = 2.99AVQFQDD88 pKa = 4.99LATDD92 pKa = 3.54YY93 pKa = 11.49SKK95 pKa = 11.5DD96 pKa = 3.3HH97 pKa = 6.58SEE99 pKa = 3.92WPGFSGDD106 pKa = 5.08AVDD109 pKa = 5.31ALNLAPVHH117 pKa = 6.4GDD119 pKa = 3.13NVVGSIPGSTSNEE132 pKa = 3.58ADD134 pKa = 4.23DD135 pKa = 5.39DD136 pKa = 4.43DD137 pKa = 6.3GYY139 pKa = 11.52YY140 pKa = 10.78SSDD143 pKa = 4.92ASLLWW148 pKa = 4.23

Molecular weight: 16.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H3JND3|A0A2H3JND3_WOLCO RRM domain-containing protein OS=Wolfiporia cocos (strain MD-104) OX=742152 GN=WOLCODRAFT_110020 PE=4 SV=1
MM1 pKa = 7.45PRR3 pKa = 11.84IPLSLSRR10 pKa = 11.84LLARR14 pKa = 11.84PPTLPFLAPRR24 pKa = 11.84AAAATSSAPAFSTLLHH40 pKa = 6.86RR41 pKa = 11.84STPSASRR48 pKa = 11.84TSRR51 pKa = 11.84ANGPLLLLAGLAARR65 pKa = 11.84SPILSMVMQARR76 pKa = 11.84HH77 pKa = 5.99RR78 pKa = 11.84SRR80 pKa = 11.84GTEE83 pKa = 3.82YY84 pKa = 10.69QPSQRR89 pKa = 11.84KK90 pKa = 8.85RR91 pKa = 11.84KK92 pKa = 9.17RR93 pKa = 11.84KK94 pKa = 9.56HH95 pKa = 5.55GFLARR100 pKa = 11.84KK101 pKa = 9.49RR102 pKa = 11.84SLGGQKK108 pKa = 9.58ILLRR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84AKK116 pKa = 9.66GRR118 pKa = 11.84RR119 pKa = 11.84FLTHH123 pKa = 6.7

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12197

0

12197

4997340

37

5033

409.7

45.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.569 ± 0.026

1.312 ± 0.01

5.579 ± 0.017

6.007 ± 0.026

3.408 ± 0.014

6.476 ± 0.02

2.616 ± 0.013

4.657 ± 0.015

4.1 ± 0.021

9.104 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.008

3.123 ± 0.014

6.545 ± 0.027

3.768 ± 0.014

6.74 ± 0.023

8.334 ± 0.026

5.926 ± 0.015

6.439 ± 0.018

1.453 ± 0.007

2.681 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski