Lucifera butyrica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Lucifera

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5043 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498RH87|A0A498RH87_9FIRM AAA_31 domain-containing protein (Fragment) OS=Lucifera butyrica OX=1351585 GN=LUCI_4742 PE=4 SV=1
MM1 pKa = 7.63ASSTSSISLSTTSSSALGTTHH22 pKa = 7.87LSNLVSGLDD31 pKa = 3.39VDD33 pKa = 4.81ALVSAQILADD43 pKa = 4.52SIPLTLLQNTVAKK56 pKa = 9.18LTAQKK61 pKa = 10.77SDD63 pKa = 3.55YY64 pKa = 9.15QTLEE68 pKa = 3.79TDD70 pKa = 3.77LQTLQYY76 pKa = 11.13ALQDD80 pKa = 3.81LTYY83 pKa = 9.19STAFSTRR90 pKa = 11.84TATSSDD96 pKa = 3.58EE97 pKa = 4.3KK98 pKa = 11.27VVTASTKK105 pKa = 8.81TGASAGSYY113 pKa = 9.87SINVSQLATTSSVTGTALSSFNDD136 pKa = 3.86GTKK139 pKa = 9.54ATLTGNVSLSGYY151 pKa = 9.58NGNDD155 pKa = 3.02STALTSLGITPGTVTINGASIVITSGDD182 pKa = 3.77TINTVLNKK190 pKa = 9.94ISASSAGVTATLNSSTGDD208 pKa = 3.27VVLTQKK214 pKa = 9.32TAGSSDD220 pKa = 4.06TITLGSDD227 pKa = 3.02GGTNLFNAFGLSSGNGTLVAGQDD250 pKa = 3.28ATDD253 pKa = 3.72AQTLTQVFGSSLQAGYY269 pKa = 10.29FSINGTVFQVNPTTDD284 pKa = 3.32TLDD287 pKa = 4.2SILSEE292 pKa = 4.26INSSTTAGVQAFYY305 pKa = 11.19NSTSKK310 pKa = 9.66TVSIVSTTAGSQGSVTLGTYY330 pKa = 10.68GSGGTDD336 pKa = 2.85SSNLLDD342 pKa = 4.06LLKK345 pKa = 10.35VTTAGGNTPAVTTGTDD361 pKa = 2.85ASVTVNGVAVAANGNSITMNGNTFALQGAGTATVTVSTNYY401 pKa = 10.28DD402 pKa = 3.61AIITKK407 pKa = 8.46VQNVVTQYY415 pKa = 11.31NSVVDD420 pKa = 4.97AINAKK425 pKa = 9.43IQEE428 pKa = 4.55APSSSSDD435 pKa = 3.34SSSSASTGDD444 pKa = 3.39LHH446 pKa = 7.57LDD448 pKa = 3.46PFLEE452 pKa = 6.0GIIQDD457 pKa = 4.11LGSFSSTVLNSPNSVYY473 pKa = 9.07QTLSQVGITTGDD485 pKa = 3.2VGQSVTDD492 pKa = 3.59SEE494 pKa = 5.01LGHH497 pKa = 7.11LSLNTDD503 pKa = 3.69TLKK506 pKa = 10.04TALEE510 pKa = 4.15TNMAGVEE517 pKa = 4.27SLFGNTTVYY526 pKa = 10.69VDD528 pKa = 3.68SEE530 pKa = 4.59KK531 pKa = 11.22VGTSSSSQLTYY542 pKa = 11.28SLAHH546 pKa = 6.64GDD548 pKa = 3.15ITGTPTVMVGSKK560 pKa = 10.1QYY562 pKa = 9.92TVVTAAQLSTDD573 pKa = 3.67DD574 pKa = 4.1TNAKK578 pKa = 10.15NSSTTIYY585 pKa = 9.64EE586 pKa = 4.29CSVDD590 pKa = 3.34ATTGKK595 pKa = 8.34ITFATGAGTFPNGSQITVSYY615 pKa = 10.71NYY617 pKa = 10.17TNTTGGSGTGIFSQMQAFINTYY639 pKa = 5.84TTVGGQFDD647 pKa = 4.12TMIGSNGSLTDD658 pKa = 3.88TISYY662 pKa = 11.19DD663 pKa = 3.41NDD665 pKa = 3.45RR666 pKa = 11.84VSEE669 pKa = 4.08MQDD672 pKa = 3.13RR673 pKa = 11.84INNEE677 pKa = 3.5QSSLYY682 pKa = 9.91TYY684 pKa = 9.71YY685 pKa = 11.59NNMLSQLQTLSSEE698 pKa = 4.08NTMVSALLSGLSSSSSSSSKK718 pKa = 10.91GG719 pKa = 3.27

Molecular weight: 73.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498R6D0|A0A498R6D0_9FIRM HD-GYP domain-containing protein OS=Lucifera butyrica OX=1351585 GN=LUCI_1975 PE=4 SV=1
MM1 pKa = 7.58TIDD4 pKa = 3.21KK5 pKa = 10.6NSRR8 pKa = 11.84RR9 pKa = 11.84NSRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84LATRR18 pKa = 11.84TAIHH22 pKa = 6.31FVLPFAIQTAIHH34 pKa = 6.34FVLPLARR41 pKa = 11.84LPALPLLRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84LAILTAGHH59 pKa = 6.35GRR61 pKa = 11.84FFATPAAASHH71 pKa = 5.77GRR73 pKa = 11.84FLAFLTAQIAARR85 pKa = 11.84APGTKK90 pKa = 9.45TNFVRR95 pKa = 11.84KK96 pKa = 9.46YY97 pKa = 9.77RR98 pKa = 11.84NQSNRR103 pKa = 11.84KK104 pKa = 6.13TADD107 pKa = 3.01AVFLLDD113 pKa = 4.6SYY115 pKa = 11.41SGHH118 pKa = 6.26NPGAASSLQRR128 pKa = 11.84FLRR131 pKa = 11.84LSQRR135 pKa = 11.84GNQRR139 pKa = 11.84LPSRR143 pKa = 11.84FGRR146 pKa = 11.84HH147 pKa = 4.15KK148 pKa = 9.75LHH150 pKa = 6.99RR151 pKa = 11.84RR152 pKa = 11.84FHH154 pKa = 6.03LRR156 pKa = 11.84QHH158 pKa = 6.37AAGSKK163 pKa = 10.16LPLRR167 pKa = 11.84NIFFRR172 pKa = 11.84FFQTHH177 pKa = 5.29LRR179 pKa = 3.87

Molecular weight: 20.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5043

0

5043

1511077

23

6215

299.6

33.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.146 ± 0.046

1.215 ± 0.016

4.789 ± 0.026

6.168 ± 0.036

4.037 ± 0.028

7.654 ± 0.04

1.881 ± 0.016

7.217 ± 0.033

5.643 ± 0.034

9.99 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.016

4.06 ± 0.026

4.066 ± 0.023

3.849 ± 0.023

4.965 ± 0.032

5.391 ± 0.025

5.377 ± 0.027

7.397 ± 0.033

1.05 ± 0.014

3.367 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski