Bacteriovorax sp. DB6_IX

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Bacteriovoracales; Bacteriovoracaceae; Bacteriovorax; unclassified Bacteriovorax

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0S299|T0S299_9PROT Uncharacterized protein OS=Bacteriovorax sp. DB6_IX OX=1353530 GN=M901_0912 PE=4 SV=1
VV1 pKa = 6.72VLEE4 pKa = 4.48EE5 pKa = 4.26EE6 pKa = 4.73EE7 pKa = 3.59ITYY10 pKa = 9.89IYY12 pKa = 9.82TFKK15 pKa = 10.92KK16 pKa = 9.97GDD18 pKa = 3.41SSITANVAVSDD29 pKa = 4.11PLYY32 pKa = 10.82FCDD35 pKa = 3.48VTFKK39 pKa = 10.83KK40 pKa = 10.73DD41 pKa = 3.33EE42 pKa = 3.99NDD44 pKa = 3.6YY45 pKa = 11.24FSLKK49 pKa = 10.31PYY51 pKa = 10.72LKK53 pKa = 9.55TLGEE57 pKa = 4.13NTEE60 pKa = 4.11MEE62 pKa = 4.37QLFDD66 pKa = 4.19FYY68 pKa = 10.57MDD70 pKa = 3.65EE71 pKa = 5.47KK72 pKa = 10.67IDD74 pKa = 3.95DD75 pKa = 3.82EE76 pKa = 4.88QYY78 pKa = 11.14VLGYY82 pKa = 11.08LNLFKK87 pKa = 10.71KK88 pKa = 10.49YY89 pKa = 10.38LSEE92 pKa = 4.88SDD94 pKa = 3.55VQMVLSGEE102 pKa = 4.48FWPDD106 pKa = 3.19VPDD109 pKa = 3.82EE110 pKa = 4.14EE111 pKa = 4.81

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0T3I7|T0T3I7_9PROT Pyridoxine 5'-phosphate synthase OS=Bacteriovorax sp. DB6_IX OX=1353530 GN=pdxJ PE=3 SV=1
MM1 pKa = 7.62ILLIALSILLSVLTFSFSKK20 pKa = 10.55LNSIGAIRR28 pKa = 11.84SSAIITLISCGIIMLLRR45 pKa = 11.84TRR47 pKa = 11.84WNFDD51 pKa = 3.05SEE53 pKa = 4.92TYY55 pKa = 9.34MALSFGASFVGMSCPTRR72 pKa = 11.84FGVKK76 pKa = 10.13SIAFGGLFFGIFFLNLVPLLTGFGGALGTSAFLAISLVHH115 pKa = 6.83LILSGARR122 pKa = 11.84HH123 pKa = 5.93LLFRR127 pKa = 11.84SCC129 pKa = 5.05

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3188

0

3188

893457

31

3867

280.3

31.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.111 ± 0.044

1.086 ± 0.018

5.695 ± 0.041

7.174 ± 0.054

5.14 ± 0.038

6.254 ± 0.069

2.049 ± 0.02

7.281 ± 0.046

8.323 ± 0.065

9.725 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.022

5.075 ± 0.036

3.222 ± 0.025

3.346 ± 0.027

4.053 ± 0.034

7.24 ± 0.045

5.076 ± 0.059

6.138 ± 0.036

0.91 ± 0.015

3.658 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski