Caulobacter phage Lullwater
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A291LB23|A0A291LB23_9CAUD DNA helicase OS=Caulobacter phage Lullwater OX=2024607 GN=Lull_017 PE=4 SV=1
MM1 pKa = 7.68 LGPSVYY7 pKa = 10.55 LPVGVQLLKK16 pKa = 10.93 DD17 pKa = 3.02 IGLNVDD23 pKa = 3.6 PPKK26 pKa = 11.12 GRR28 pKa = 11.84 DD29 pKa = 3.04 PWDD32 pKa = 3.96 YY33 pKa = 10.07 ICEE36 pKa = 4.32 DD37 pKa = 3.66 EE38 pKa = 5.58 SVTFTDD44 pKa = 3.25 QTDD47 pKa = 3.53 RR48 pKa = 11.84 DD49 pKa = 3.61 ILEE52 pKa = 3.89 AHH54 pKa = 7.14 GIVVDD59 pKa = 4.24 CTPVDD64 pKa = 3.08 AFMNRR69 pKa = 11.84 GYY71 pKa = 11.33 GFLLTEE77 pKa = 4.4 EE78 pKa = 4.63 DD79 pKa = 3.51
Molecular weight: 8.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.996
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.592
ProMoST 3.923
Dawson 3.808
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.935
Patrickios 2.994
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.879
Protein with the highest isoelectric point:
>tr|A0A291LC12|A0A291LC12_9CAUD Uncharacterized protein OS=Caulobacter phage Lullwater OX=2024607 GN=Lull_012 PE=4 SV=1
MM1 pKa = 8.21 DD2 pKa = 5.71 DD3 pKa = 3.68 FASFGIGLVAVVFAAVLVWRR23 pKa = 11.84 EE24 pKa = 3.55 RR25 pKa = 11.84 QAYY28 pKa = 7.1 MWRR31 pKa = 11.84 QRR33 pKa = 11.84 QIAKK37 pKa = 10.49 GKK39 pKa = 9.37 DD40 pKa = 3.16 LRR42 pKa = 11.84 PP43 pKa = 3.54
Molecular weight: 5.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.269
IPC2_protein 9.472
IPC_protein 10.116
Toseland 10.452
ProMoST 10.116
Dawson 10.57
Bjellqvist 10.248
Wikipedia 10.76
Rodwell 10.804
Grimsley 10.628
Solomon 10.687
Lehninger 10.657
Nozaki 10.409
DTASelect 10.248
Thurlkill 10.452
EMBOSS 10.847
Sillero 10.482
Patrickios 10.716
IPC_peptide 10.687
IPC2_peptide 8.931
IPC2.peptide.svr19 8.701
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
52
0
52
13664
40
1317
262.8
29.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.992 ± 0.532
0.746 ± 0.128
6.279 ± 0.186
5.782 ± 0.335
3.476 ± 0.172
7.362 ± 0.294
1.778 ± 0.17
4.537 ± 0.191
5.657 ± 0.269
8.277 ± 0.277
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.605 ± 0.208
4.215 ± 0.189
3.967 ± 0.221
4.311 ± 0.306
5.708 ± 0.292
6.038 ± 0.257
6.316 ± 0.436
6.828 ± 0.321
1.566 ± 0.164
3.557 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here