Amino acid dipepetide frequency for Caulobacter phage Lullwater

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.126AlaAla: 14.126 ± 2.1
1.025AlaCys: 1.025 ± 0.277
5.782AlaAsp: 5.782 ± 0.702
7.319AlaGlu: 7.319 ± 0.77
3.513AlaPhe: 3.513 ± 0.4
7.539AlaGly: 7.539 ± 1.062
2.123AlaHis: 2.123 ± 0.48
5.416AlaIle: 5.416 ± 0.73
6.66AlaLys: 6.66 ± 0.677
8.856AlaLeu: 8.856 ± 0.782
2.928AlaMet: 2.928 ± 0.494
4.538AlaAsn: 4.538 ± 0.712
3.733AlaPro: 3.733 ± 0.696
5.636AlaGln: 5.636 ± 1.106
6.075AlaArg: 6.075 ± 0.797
5.928AlaSer: 5.928 ± 0.818
6.148AlaThr: 6.148 ± 0.938
6.587AlaVal: 6.587 ± 0.618
2.196AlaTrp: 2.196 ± 0.413
3.806AlaTyr: 3.806 ± 0.479
0.0AlaXaa: 0.0 ± 0.0
Cys
0.951CysAla: 0.951 ± 0.335
0.146CysCys: 0.146 ± 0.111
0.146CysAsp: 0.146 ± 0.144
0.439CysGlu: 0.439 ± 0.178
0.512CysPhe: 0.512 ± 0.217
0.439CysGly: 0.439 ± 0.234
0.146CysHis: 0.146 ± 0.131
0.659CysIle: 0.659 ± 0.245
0.22CysLys: 0.22 ± 0.127
0.732CysLeu: 0.732 ± 0.284
0.22CysMet: 0.22 ± 0.117
0.366CysAsn: 0.366 ± 0.188
0.586CysPro: 0.586 ± 0.18
0.293CysGln: 0.293 ± 0.135
0.073CysArg: 0.073 ± 0.066
0.512CysSer: 0.512 ± 0.184
0.293CysThr: 0.293 ± 0.155
0.439CysVal: 0.439 ± 0.169
0.0CysTrp: 0.0 ± 0.0
0.293CysTyr: 0.293 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
6.587AspAla: 6.587 ± 0.755
0.659AspCys: 0.659 ± 0.224
3.513AspAsp: 3.513 ± 0.561
3.074AspGlu: 3.074 ± 0.662
3.001AspPhe: 3.001 ± 0.689
4.977AspGly: 4.977 ± 0.635
1.171AspHis: 1.171 ± 0.335
3.586AspIle: 3.586 ± 0.546
3.66AspLys: 3.66 ± 0.533
4.977AspLeu: 4.977 ± 0.682
1.903AspMet: 1.903 ± 0.353
1.61AspAsn: 1.61 ± 0.313
3.367AspPro: 3.367 ± 0.58
2.854AspGln: 2.854 ± 0.46
3.66AspArg: 3.66 ± 0.53
2.415AspSer: 2.415 ± 0.454
4.538AspThr: 4.538 ± 1.115
4.172AspVal: 4.172 ± 0.59
0.586AspTrp: 0.586 ± 0.281
2.488AspTyr: 2.488 ± 0.452
0.0AspXaa: 0.0 ± 0.0
Glu
6.221GluAla: 6.221 ± 0.979
0.586GluCys: 0.586 ± 0.274
4.831GluAsp: 4.831 ± 0.751
4.831GluGlu: 4.831 ± 0.649
2.123GluPhe: 2.123 ± 0.603
2.562GluGly: 2.562 ± 0.529
1.317GluHis: 1.317 ± 0.402
2.928GluIle: 2.928 ± 0.39
3.367GluLys: 3.367 ± 0.704
5.562GluLeu: 5.562 ± 0.921
1.244GluMet: 1.244 ± 0.423
1.683GluAsn: 1.683 ± 0.376
1.537GluPro: 1.537 ± 0.364
3.44GluGln: 3.44 ± 0.638
2.781GluArg: 2.781 ± 0.447
1.976GluSer: 1.976 ± 0.409
2.708GluThr: 2.708 ± 0.475
4.245GluVal: 4.245 ± 0.666
1.317GluTrp: 1.317 ± 0.368
2.562GluTyr: 2.562 ± 0.398
0.0GluXaa: 0.0 ± 0.0
Phe
3.22PheAla: 3.22 ± 0.624
0.366PheCys: 0.366 ± 0.17
2.781PheAsp: 2.781 ± 0.31
1.83PheGlu: 1.83 ± 0.424
0.878PhePhe: 0.878 ± 0.318
2.488PheGly: 2.488 ± 0.442
0.586PheHis: 0.586 ± 0.269
1.903PheIle: 1.903 ± 0.342
1.464PheLys: 1.464 ± 0.302
2.854PheLeu: 2.854 ± 0.523
0.512PheMet: 0.512 ± 0.199
2.708PheAsn: 2.708 ± 0.51
1.244PhePro: 1.244 ± 0.326
1.464PheGln: 1.464 ± 0.309
1.83PheArg: 1.83 ± 0.329
2.196PheSer: 2.196 ± 0.378
2.342PheThr: 2.342 ± 0.386
2.415PheVal: 2.415 ± 0.448
0.293PheTrp: 0.293 ± 0.166
1.391PheTyr: 1.391 ± 0.307
0.0PheXaa: 0.0 ± 0.0
Gly
7.026GlyAla: 7.026 ± 0.867
0.659GlyCys: 0.659 ± 0.231
4.611GlyAsp: 4.611 ± 0.602
3.367GlyGlu: 3.367 ± 0.686
2.854GlyPhe: 2.854 ± 0.569
6.221GlyGly: 6.221 ± 0.876
1.537GlyHis: 1.537 ± 0.455
3.074GlyIle: 3.074 ± 0.53
4.904GlyLys: 4.904 ± 0.59
5.123GlyLeu: 5.123 ± 0.796
2.269GlyMet: 2.269 ± 0.45
3.513GlyAsn: 3.513 ± 0.454
2.123GlyPro: 2.123 ± 0.479
2.562GlyGln: 2.562 ± 0.465
4.611GlyArg: 4.611 ± 0.71
5.782GlySer: 5.782 ± 0.761
4.245GlyThr: 4.245 ± 0.469
5.343GlyVal: 5.343 ± 0.818
1.025GlyTrp: 1.025 ± 0.355
2.781GlyTyr: 2.781 ± 0.452
0.0GlyXaa: 0.0 ± 0.0
His
1.244HisAla: 1.244 ± 0.301
0.073HisCys: 0.073 ± 0.071
1.537HisAsp: 1.537 ± 0.428
1.098HisGlu: 1.098 ± 0.246
0.659HisPhe: 0.659 ± 0.217
1.464HisGly: 1.464 ± 0.342
0.22HisHis: 0.22 ± 0.134
0.659HisIle: 0.659 ± 0.255
0.951HisLys: 0.951 ± 0.273
1.683HisLeu: 1.683 ± 0.423
0.586HisMet: 0.586 ± 0.191
0.805HisAsn: 0.805 ± 0.231
0.951HisPro: 0.951 ± 0.241
0.439HisGln: 0.439 ± 0.159
0.878HisArg: 0.878 ± 0.313
1.244HisSer: 1.244 ± 0.375
1.244HisThr: 1.244 ± 0.387
1.171HisVal: 1.171 ± 0.364
0.22HisTrp: 0.22 ± 0.112
0.659HisTyr: 0.659 ± 0.244
0.0HisXaa: 0.0 ± 0.0
Ile
4.684IleAla: 4.684 ± 0.511
0.293IleCys: 0.293 ± 0.158
2.196IleAsp: 2.196 ± 0.464
3.22IleGlu: 3.22 ± 0.623
0.805IlePhe: 0.805 ± 0.209
3.952IleGly: 3.952 ± 0.498
0.732IleHis: 0.732 ± 0.222
1.903IleIle: 1.903 ± 0.369
3.001IleLys: 3.001 ± 0.612
3.074IleLeu: 3.074 ± 0.543
1.171IleMet: 1.171 ± 0.329
1.83IleAsn: 1.83 ± 0.37
2.415IlePro: 2.415 ± 0.507
2.049IleGln: 2.049 ± 0.413
2.854IleArg: 2.854 ± 0.504
2.196IleSer: 2.196 ± 0.413
3.44IleThr: 3.44 ± 0.706
3.586IleVal: 3.586 ± 0.707
0.366IleTrp: 0.366 ± 0.174
1.61IleTyr: 1.61 ± 0.444
0.0IleXaa: 0.0 ± 0.0
Lys
7.246LysAla: 7.246 ± 1.086
0.22LysCys: 0.22 ± 0.124
4.465LysAsp: 4.465 ± 0.468
3.001LysGlu: 3.001 ± 0.562
2.049LysPhe: 2.049 ± 0.416
3.294LysGly: 3.294 ± 0.463
0.805LysHis: 0.805 ± 0.339
1.61LysIle: 1.61 ± 0.358
3.44LysLys: 3.44 ± 0.59
5.343LysLeu: 5.343 ± 0.624
1.025LysMet: 1.025 ± 0.268
2.196LysAsn: 2.196 ± 0.398
2.635LysPro: 2.635 ± 0.446
2.415LysGln: 2.415 ± 0.492
2.928LysArg: 2.928 ± 0.435
3.001LysSer: 3.001 ± 0.544
3.586LysThr: 3.586 ± 0.525
4.245LysVal: 4.245 ± 0.57
1.098LysTrp: 1.098 ± 0.328
1.976LysTyr: 1.976 ± 0.361
0.0LysXaa: 0.0 ± 0.0
Leu
9.368LeuAla: 9.368 ± 0.772
0.586LeuCys: 0.586 ± 0.231
5.489LeuAsp: 5.489 ± 0.545
5.416LeuGlu: 5.416 ± 0.674
2.635LeuPhe: 2.635 ± 0.461
5.343LeuGly: 5.343 ± 0.829
1.757LeuHis: 1.757 ± 0.427
3.147LeuIle: 3.147 ± 0.722
4.757LeuLys: 4.757 ± 0.662
6.148LeuLeu: 6.148 ± 0.698
1.683LeuMet: 1.683 ± 0.295
3.513LeuAsn: 3.513 ± 0.567
3.586LeuPro: 3.586 ± 0.559
2.854LeuGln: 2.854 ± 0.603
6.221LeuArg: 6.221 ± 0.757
5.27LeuSer: 5.27 ± 0.565
6.368LeuThr: 6.368 ± 0.857
4.318LeuVal: 4.318 ± 0.597
1.317LeuTrp: 1.317 ± 0.377
3.001LeuTyr: 3.001 ± 0.592
0.0LeuXaa: 0.0 ± 0.0
Met
3.074MetAla: 3.074 ± 0.565
0.073MetCys: 0.073 ± 0.072
1.391MetAsp: 1.391 ± 0.331
1.025MetGlu: 1.025 ± 0.344
0.512MetPhe: 0.512 ± 0.155
2.342MetGly: 2.342 ± 0.568
0.22MetHis: 0.22 ± 0.113
0.732MetIle: 0.732 ± 0.218
1.61MetLys: 1.61 ± 0.378
2.342MetLeu: 2.342 ± 0.432
0.878MetMet: 0.878 ± 0.223
1.098MetAsn: 1.098 ± 0.253
0.732MetPro: 0.732 ± 0.234
1.391MetGln: 1.391 ± 0.284
1.83MetArg: 1.83 ± 0.381
2.049MetSer: 2.049 ± 0.358
2.049MetThr: 2.049 ± 0.489
1.757MetVal: 1.757 ± 0.441
0.439MetTrp: 0.439 ± 0.184
0.512MetTyr: 0.512 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
5.562AsnAla: 5.562 ± 0.928
0.293AsnCys: 0.293 ± 0.139
2.342AsnAsp: 2.342 ± 0.421
1.537AsnGlu: 1.537 ± 0.285
1.244AsnPhe: 1.244 ± 0.362
4.172AsnGly: 4.172 ± 0.511
0.659AsnHis: 0.659 ± 0.216
2.781AsnIle: 2.781 ± 0.614
2.196AsnLys: 2.196 ± 0.345
3.586AsnLeu: 3.586 ± 0.481
1.464AsnMet: 1.464 ± 0.361
1.537AsnAsn: 1.537 ± 0.365
2.049AsnPro: 2.049 ± 0.385
1.976AsnGln: 1.976 ± 0.442
1.83AsnArg: 1.83 ± 0.452
2.488AsnSer: 2.488 ± 0.4
2.269AsnThr: 2.269 ± 0.52
2.562AsnVal: 2.562 ± 0.399
0.512AsnTrp: 0.512 ± 0.201
1.098AsnTyr: 1.098 ± 0.269
0.0AsnXaa: 0.0 ± 0.0
Pro
3.66ProAla: 3.66 ± 0.578
0.146ProCys: 0.146 ± 0.108
2.342ProAsp: 2.342 ± 0.486
2.196ProGlu: 2.196 ± 0.39
1.61ProPhe: 1.61 ± 0.332
3.586ProGly: 3.586 ± 0.441
0.732ProHis: 0.732 ± 0.258
1.683ProIle: 1.683 ± 0.345
2.635ProLys: 2.635 ± 0.538
3.147ProLeu: 3.147 ± 0.488
1.098ProMet: 1.098 ± 0.228
1.83ProAsn: 1.83 ± 0.313
1.391ProPro: 1.391 ± 0.381
1.317ProGln: 1.317 ± 0.282
1.83ProArg: 1.83 ± 0.429
2.928ProSer: 2.928 ± 0.458
3.074ProThr: 3.074 ± 0.736
2.928ProVal: 2.928 ± 0.656
0.366ProTrp: 0.366 ± 0.158
1.171ProTyr: 1.171 ± 0.504
0.0ProXaa: 0.0 ± 0.0
Gln
7.905GlnAla: 7.905 ± 1.11
0.073GlnCys: 0.073 ± 0.082
1.757GlnAsp: 1.757 ± 0.452
2.928GlnGlu: 2.928 ± 0.415
1.025GlnPhe: 1.025 ± 0.247
3.001GlnGly: 3.001 ± 0.623
0.586GlnHis: 0.586 ± 0.267
1.757GlnIle: 1.757 ± 0.386
2.342GlnLys: 2.342 ± 0.404
3.147GlnLeu: 3.147 ± 0.431
1.171GlnMet: 1.171 ± 0.293
1.537GlnAsn: 1.537 ± 0.319
1.171GlnPro: 1.171 ± 0.357
2.562GlnGln: 2.562 ± 0.675
2.562GlnArg: 2.562 ± 0.552
1.83GlnSer: 1.83 ± 0.412
2.342GlnThr: 2.342 ± 0.426
3.44GlnVal: 3.44 ± 0.503
0.439GlnTrp: 0.439 ± 0.169
1.537GlnTyr: 1.537 ± 0.45
0.0GlnXaa: 0.0 ± 0.0
Arg
5.05ArgAla: 5.05 ± 0.73
0.512ArgCys: 0.512 ± 0.205
4.172ArgAsp: 4.172 ± 0.54
3.733ArgGlu: 3.733 ± 0.5
1.464ArgPhe: 1.464 ± 0.446
4.025ArgGly: 4.025 ± 0.596
0.878ArgHis: 0.878 ± 0.485
2.415ArgIle: 2.415 ± 0.461
3.074ArgLys: 3.074 ± 0.558
5.562ArgLeu: 5.562 ± 0.616
1.464ArgMet: 1.464 ± 0.37
2.488ArgAsn: 2.488 ± 0.401
1.903ArgPro: 1.903 ± 0.451
2.049ArgGln: 2.049 ± 0.42
3.952ArgArg: 3.952 ± 0.525
3.367ArgSer: 3.367 ± 0.626
2.488ArgThr: 2.488 ± 0.33
4.684ArgVal: 4.684 ± 0.546
1.317ArgTrp: 1.317 ± 0.329
2.415ArgTyr: 2.415 ± 0.428
0.0ArgXaa: 0.0 ± 0.0
Ser
5.27SerAla: 5.27 ± 0.834
0.512SerCys: 0.512 ± 0.209
4.099SerAsp: 4.099 ± 0.646
3.733SerGlu: 3.733 ± 0.455
2.928SerPhe: 2.928 ± 0.486
4.245SerGly: 4.245 ± 0.778
1.317SerHis: 1.317 ± 0.437
2.708SerIle: 2.708 ± 0.461
2.562SerLys: 2.562 ± 0.451
5.416SerLeu: 5.416 ± 0.797
1.683SerMet: 1.683 ± 0.38
2.049SerAsn: 2.049 ± 0.488
2.196SerPro: 2.196 ± 0.36
2.342SerGln: 2.342 ± 0.598
4.391SerArg: 4.391 ± 0.863
3.733SerSer: 3.733 ± 0.71
3.806SerThr: 3.806 ± 0.566
4.172SerVal: 4.172 ± 0.935
0.951SerTrp: 0.951 ± 0.268
2.269SerTyr: 2.269 ± 0.466
0.0SerXaa: 0.0 ± 0.0
Thr
6.441ThrAla: 6.441 ± 1.043
0.22ThrCys: 0.22 ± 0.143
3.074ThrAsp: 3.074 ± 0.365
3.147ThrGlu: 3.147 ± 0.515
1.757ThrPhe: 1.757 ± 0.412
5.343ThrGly: 5.343 ± 0.598
0.878ThrHis: 0.878 ± 0.297
2.928ThrIle: 2.928 ± 0.668
3.879ThrLys: 3.879 ± 0.469
5.855ThrLeu: 5.855 ± 0.664
1.464ThrMet: 1.464 ± 0.368
2.854ThrAsn: 2.854 ± 0.665
3.294ThrPro: 3.294 ± 0.583
2.342ThrGln: 2.342 ± 0.326
2.488ThrArg: 2.488 ± 0.285
4.977ThrSer: 4.977 ± 1.454
4.465ThrThr: 4.465 ± 1.112
4.025ThrVal: 4.025 ± 0.771
0.951ThrTrp: 0.951 ± 0.287
2.781ThrTyr: 2.781 ± 0.661
0.0ThrXaa: 0.0 ± 0.0
Val
6.514ValAla: 6.514 ± 0.541
0.293ValCys: 0.293 ± 0.161
3.952ValAsp: 3.952 ± 0.596
3.586ValGlu: 3.586 ± 0.533
2.562ValPhe: 2.562 ± 0.359
5.416ValGly: 5.416 ± 0.715
1.244ValHis: 1.244 ± 0.34
3.66ValIle: 3.66 ± 0.497
3.294ValLys: 3.294 ± 0.716
4.977ValLeu: 4.977 ± 0.61
1.976ValMet: 1.976 ± 0.413
3.586ValAsn: 3.586 ± 0.489
2.928ValPro: 2.928 ± 0.521
2.781ValGln: 2.781 ± 0.472
4.099ValArg: 4.099 ± 0.618
5.123ValSer: 5.123 ± 0.835
4.245ValThr: 4.245 ± 0.763
4.538ValVal: 4.538 ± 0.576
1.391ValTrp: 1.391 ± 0.391
2.123ValTyr: 2.123 ± 0.446
0.0ValXaa: 0.0 ± 0.0
Trp
1.757TrpAla: 1.757 ± 0.391
0.293TrpCys: 0.293 ± 0.168
1.171TrpAsp: 1.171 ± 0.264
0.586TrpGlu: 0.586 ± 0.201
1.025TrpPhe: 1.025 ± 0.278
0.878TrpGly: 0.878 ± 0.22
0.22TrpHis: 0.22 ± 0.133
0.293TrpIle: 0.293 ± 0.146
0.805TrpLys: 0.805 ± 0.262
1.683TrpLeu: 1.683 ± 0.392
0.366TrpMet: 0.366 ± 0.176
1.025TrpAsn: 1.025 ± 0.373
0.732TrpPro: 0.732 ± 0.236
0.512TrpGln: 0.512 ± 0.206
0.732TrpArg: 0.732 ± 0.251
1.171TrpSer: 1.171 ± 0.291
1.025TrpThr: 1.025 ± 0.254
0.878TrpVal: 0.878 ± 0.286
0.146TrpTrp: 0.146 ± 0.085
0.366TrpTyr: 0.366 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.025TyrAla: 4.025 ± 0.428
0.439TyrCys: 0.439 ± 0.18
3.147TyrAsp: 3.147 ± 0.67
1.757TyrGlu: 1.757 ± 0.34
1.61TyrPhe: 1.61 ± 0.42
2.342TyrGly: 2.342 ± 0.355
0.659TyrHis: 0.659 ± 0.235
1.537TyrIle: 1.537 ± 0.337
1.757TyrLys: 1.757 ± 0.345
2.708TyrLeu: 2.708 ± 0.363
0.878TyrMet: 0.878 ± 0.186
1.391TyrAsn: 1.391 ± 0.59
1.098TyrPro: 1.098 ± 0.362
1.83TyrGln: 1.83 ± 0.441
1.464TyrArg: 1.464 ± 0.376
2.196TyrSer: 2.196 ± 0.372
2.488TyrThr: 2.488 ± 0.648
2.781TyrVal: 2.781 ± 0.547
0.732TyrTrp: 0.732 ± 0.256
0.732TyrTyr: 0.732 ± 0.286
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (13664 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski