Paraburkholderia caffeinilytica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5423 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BGF7|A0A346BGF7_9BURK ATP synthase gamma chain OS=Paraburkholderia caffeinilytica OX=1761016 GN=atpG PE=3 SV=1
MM1 pKa = 6.7EE2 pKa = 4.1QFYY5 pKa = 10.98EE6 pKa = 4.58GMAEE10 pKa = 3.63IFEE13 pKa = 4.59IDD15 pKa = 3.45VSQITPEE22 pKa = 4.89LDD24 pKa = 3.43LQEE27 pKa = 5.27HH28 pKa = 6.07NWDD31 pKa = 3.51SLAVVSTIALVDD43 pKa = 3.4DD44 pKa = 5.08CFNVMLSGQALNNCNTIADD63 pKa = 4.0IEE65 pKa = 4.31ALISTAKK72 pKa = 10.18KK73 pKa = 10.12AAA75 pKa = 3.64

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BGV8|A0A346BGV8_9BURK ATP-dependent protease subunit HslV OS=Paraburkholderia caffeinilytica OX=1761016 GN=hslV PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5423

0

5423

1771409

37

3896

326.6

35.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.7 ± 0.043

0.896 ± 0.01

5.373 ± 0.025

5.138 ± 0.034

3.744 ± 0.024

8.218 ± 0.035

2.299 ± 0.016

4.79 ± 0.024

3.131 ± 0.028

10.195 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.017

2.908 ± 0.024

5.008 ± 0.022

3.649 ± 0.02

6.683 ± 0.035

5.789 ± 0.027

5.544 ± 0.033

7.776 ± 0.025

1.347 ± 0.015

2.445 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski