Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium; Agrobacterium tumefaciens complex; Agrobacterium fabrum

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5344 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7CXS1|Q7CXS1_AGRFC Uncharacterized protein OS=Agrobacterium fabrum (strain C58 / ATCC 33970) OX=176299 GN=Atu2146 PE=4 SV=2
MM1 pKa = 7.05KK2 pKa = 9.67TLIVTSLLALSASTAMAADD21 pKa = 3.7AVYY24 pKa = 7.47EE25 pKa = 4.53TPAPPVAQEE34 pKa = 4.02TLPVFTWSGPYY45 pKa = 10.34LGIQGGAGWANGDD58 pKa = 4.05FSAGGPVVSDD68 pKa = 4.23DD69 pKa = 3.92FNGGILGAFAGYY81 pKa = 10.56NYY83 pKa = 10.18QFDD86 pKa = 4.31NNMVLGIEE94 pKa = 4.47GDD96 pKa = 3.56VDD98 pKa = 4.49YY99 pKa = 11.53NWNDD103 pKa = 3.04NDD105 pKa = 3.54YY106 pKa = 11.35SGIKK110 pKa = 10.23VGTDD114 pKa = 2.83WQGSVRR120 pKa = 11.84GRR122 pKa = 11.84VGYY125 pKa = 10.8AFDD128 pKa = 3.93HH129 pKa = 6.09ALIYY133 pKa = 9.3ATAGWTATRR142 pKa = 11.84GFIEE146 pKa = 4.74TPVGDD151 pKa = 5.17DD152 pKa = 2.85KK153 pKa = 11.72ATFNGYY159 pKa = 7.0TVGAGVDD166 pKa = 3.5YY167 pKa = 11.31AFTDD171 pKa = 3.55NVFGRR176 pKa = 11.84LEE178 pKa = 3.82YY179 pKa = 10.33RR180 pKa = 11.84YY181 pKa = 10.46NDD183 pKa = 3.79YY184 pKa = 11.23GDD186 pKa = 3.93KK187 pKa = 10.89DD188 pKa = 3.33IFGINTDD195 pKa = 4.43FDD197 pKa = 3.52QHH199 pKa = 4.79TVKK202 pKa = 10.85VGLGVKK208 pKa = 9.56FF209 pKa = 3.72

Molecular weight: 22.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q44452|TRAM_AGRFC Transcriptional repressor TraM OS=Agrobacterium fabrum (strain C58 / ATCC 33970) OX=176299 GN=traM PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.84LVRR13 pKa = 11.84KK14 pKa = 9.15RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATAGGRR29 pKa = 11.84KK30 pKa = 8.88VLAARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.99

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5344

0

5344

1660809

33

2831

310.8

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.572 ± 0.046

0.798 ± 0.01

5.589 ± 0.026

5.816 ± 0.031

4.053 ± 0.023

8.266 ± 0.033

2.01 ± 0.015

5.797 ± 0.028

3.892 ± 0.03

9.925 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.015

2.986 ± 0.018

4.782 ± 0.023

3.125 ± 0.02

6.564 ± 0.032

5.946 ± 0.024

5.375 ± 0.022

7.338 ± 0.026

1.252 ± 0.013

2.313 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski