Heterocephalus glaber (Naked mole rat)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires;

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21449 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G5AUA9|G5AUA9_HETGA LON peptidase and RING finger protein 1 OS=Heterocephalus glaber OX=10181 GN=GW7_13438 PE=4 SV=1
MM1 pKa = 7.86PNQSAADD8 pKa = 4.03RR9 pKa = 11.84QVCARR14 pKa = 11.84PTEE17 pKa = 4.25VSSPTEE23 pKa = 3.8HH24 pKa = 6.45TEE26 pKa = 3.86SARR29 pKa = 11.84ALATIHH35 pKa = 5.69WTPIPAPLYY44 pKa = 8.76SHH46 pKa = 7.15LVLHH50 pKa = 6.01YY51 pKa = 10.66KK52 pKa = 8.4EE53 pKa = 4.97TEE55 pKa = 4.12DD56 pKa = 4.82TGPPDD61 pKa = 3.59NGTEE65 pKa = 4.22SSGSLLRR72 pKa = 11.84DD73 pKa = 3.8PEE75 pKa = 5.77DD76 pKa = 4.22EE77 pKa = 5.86DD78 pKa = 4.44SDD80 pKa = 5.11GYY82 pKa = 11.21DD83 pKa = 3.22GEE85 pKa = 4.53EE86 pKa = 4.33YY87 pKa = 10.51DD88 pKa = 5.49VEE90 pKa = 4.24AHH92 pKa = 5.97EE93 pKa = 4.68QGQGDD98 pKa = 3.55IPKK101 pKa = 9.87FYY103 pKa = 9.91TYY105 pKa = 10.99EE106 pKa = 4.02EE107 pKa = 4.41GLSYY111 pKa = 9.92LTAEE115 pKa = 4.55GQDD118 pKa = 3.74TLEE121 pKa = 4.56MLPQSVSSQYY131 pKa = 11.78NMAGVQTEE139 pKa = 4.1DD140 pKa = 3.07SVRR143 pKa = 11.84DD144 pKa = 3.75YY145 pKa = 10.86EE146 pKa = 5.55DD147 pKa = 3.45SMEE150 pKa = 4.08VDD152 pKa = 3.52TTPGVARR159 pKa = 11.84QFEE162 pKa = 4.44DD163 pKa = 4.42ADD165 pKa = 3.59IDD167 pKa = 3.93HH168 pKa = 7.16

Molecular weight: 18.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G5BBX4|G5BBX4_HETGA G-protein coupled receptor 98 OS=Heterocephalus glaber OX=10181 GN=GW7_06052 PE=4 SV=1
MM1 pKa = 7.78APPSVPQPVLFCDD14 pKa = 4.57LPVCFQVAKK23 pKa = 9.93KK24 pKa = 10.13SSRR27 pKa = 11.84ARR29 pKa = 11.84RR30 pKa = 11.84PGPRR34 pKa = 11.84TGKK37 pKa = 10.14KK38 pKa = 9.66LSPRR42 pKa = 11.84ASPRR46 pKa = 11.84PPRR49 pKa = 11.84DD50 pKa = 3.06RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84KK54 pKa = 7.52PQQGLAVLSTNRR66 pKa = 11.84GSSPSKK72 pKa = 9.46MRR74 pKa = 11.84QGSSRR79 pKa = 11.84ALGARR84 pKa = 11.84KK85 pKa = 9.21RR86 pKa = 11.84CTRR89 pKa = 11.84RR90 pKa = 11.84GPKK93 pKa = 9.74RR94 pKa = 11.84SPAKK98 pKa = 10.17LGRR101 pKa = 11.84NRR103 pKa = 11.84STRR106 pKa = 11.84GRR108 pKa = 11.84SRR110 pKa = 11.84GRR112 pKa = 11.84RR113 pKa = 11.84APASRR118 pKa = 11.84KK119 pKa = 6.87TMPMRR124 pKa = 4.36

Molecular weight: 13.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21446

3

21449

10466552

37

36507

488.0

54.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.913 ± 0.017

2.309 ± 0.016

4.766 ± 0.012

7.028 ± 0.025

3.734 ± 0.015

6.461 ± 0.022

2.666 ± 0.013

4.402 ± 0.016

5.728 ± 0.021

10.107 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.009

3.539 ± 0.023

6.082 ± 0.025

4.751 ± 0.018

5.489 ± 0.016

8.239 ± 0.022

5.311 ± 0.014

6.155 ± 0.015

1.226 ± 0.006

2.688 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski