Acinetobacter phage SH-Ab 15599

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; unclassified Ackermannviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AWY8|A0A345AWY8_9CAUD DNA-binding protein HU-beta OS=Acinetobacter phage SH-Ab 15599 OX=2202135 GN=SHAb15599_00057 PE=3 SV=1
MM1 pKa = 7.2SWYY4 pKa = 10.4HH5 pKa = 5.46ITPLEE10 pKa = 4.15NLDD13 pKa = 4.62SILQFGLILHH23 pKa = 6.91AVPNTSDD30 pKa = 2.93NHH32 pKa = 6.76DD33 pKa = 3.95PLGHH37 pKa = 5.92YY38 pKa = 10.5VSDD41 pKa = 3.83DD42 pKa = 3.06WVQLLISDD50 pKa = 4.67TYY52 pKa = 11.37PEE54 pKa = 3.81IFKK57 pKa = 10.6YY58 pKa = 10.8DD59 pKa = 4.09EE60 pKa = 3.92IAILLVDD67 pKa = 3.76ISDD70 pKa = 4.12YY71 pKa = 10.48QTMDD75 pKa = 3.45DD76 pKa = 4.69PEE78 pKa = 4.32YY79 pKa = 11.35SSIKK83 pKa = 10.37SDD85 pKa = 4.95DD86 pKa = 3.8DD87 pKa = 3.87FQVQVVLSNVKK98 pKa = 10.55AKK100 pKa = 10.48DD101 pKa = 3.25IEE103 pKa = 4.37LLCTVDD109 pKa = 4.55FSEE112 pKa = 4.94IDD114 pKa = 3.49FNIYY118 pKa = 9.46SNKK121 pKa = 9.34RR122 pKa = 11.84LKK124 pKa = 10.78EE125 pKa = 3.63FLEE128 pKa = 4.17YY129 pKa = 10.83KK130 pKa = 9.82EE131 pKa = 4.33AQSAA135 pKa = 3.47

Molecular weight: 15.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AX54|A0A345AX54_9CAUD Uncharacterized protein OS=Acinetobacter phage SH-Ab 15599 OX=2202135 GN=SHAb15599_00131 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.38KK3 pKa = 10.19LQFLYY8 pKa = 9.52MYY10 pKa = 10.22LKK12 pKa = 10.6VFPFYY17 pKa = 11.03YY18 pKa = 9.98WATFWLMPRR27 pKa = 11.84LYY29 pKa = 10.29KK30 pKa = 10.4SYY32 pKa = 10.09PHH34 pKa = 7.44DD35 pKa = 3.61STVAVYY41 pKa = 9.14EE42 pKa = 4.14VEE44 pKa = 4.33VIRR47 pKa = 11.84FTEE50 pKa = 3.83ISSVVAHH57 pKa = 5.54SQRR60 pKa = 11.84MRR62 pKa = 11.84KK63 pKa = 7.14TKK65 pKa = 10.55NEE67 pKa = 3.49AYY69 pKa = 10.79LRR71 pKa = 11.84MRR73 pKa = 11.84LAALQIDD80 pKa = 4.61FRR82 pKa = 11.84TPAMFGISFRR92 pKa = 11.84LRR94 pKa = 11.84KK95 pKa = 10.01VGEE98 pKa = 3.9

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

177

0

177

43512

37

1603

245.8

27.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.499 ± 0.203

1.096 ± 0.07

6.293 ± 0.13

6.796 ± 0.2

4.67 ± 0.141

6.044 ± 0.227

1.871 ± 0.102

6.895 ± 0.156

7.127 ± 0.194

7.904 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.56 ± 0.091

5.883 ± 0.132

3.597 ± 0.121

3.817 ± 0.102

4.153 ± 0.125

6.706 ± 0.17

6.074 ± 0.208

6.929 ± 0.151

1.165 ± 0.059

3.921 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski