Microviridae sp. ct0DW36

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WA30|A0A5Q2WA30_9VIRU Putative minor capsid protein OS=Microviridae sp. ct0DW36 OX=2656693 PE=4 SV=1
MM1 pKa = 7.82IYY3 pKa = 10.25HH4 pKa = 7.05IFSVFDD10 pKa = 3.67YY11 pKa = 10.8GAEE14 pKa = 3.94HH15 pKa = 6.8YY16 pKa = 7.63MTPFYY21 pKa = 11.33VEE23 pKa = 3.93NEE25 pKa = 3.89KK26 pKa = 10.84LAVRR30 pKa = 11.84MFSDD34 pKa = 3.57ICQDD38 pKa = 2.96SDD40 pKa = 3.85SAIARR45 pKa = 11.84HH46 pKa = 6.1PSDD49 pKa = 4.68FSLFCLGSFNNCSGDD64 pKa = 4.08FNIHH68 pKa = 5.75PQPEE72 pKa = 4.52LVSKK76 pKa = 10.7AVSFVDD82 pKa = 3.4SRR84 pKa = 11.84SAADD88 pKa = 3.81HH89 pKa = 6.4IARR92 pKa = 11.84AVVAASDD99 pKa = 3.6SS100 pKa = 3.6

Molecular weight: 11.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W7I2|A0A5Q2W7I2_9VIRU Scaffold protein OS=Microviridae sp. ct0DW36 OX=2656693 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84PHH4 pKa = 6.94GLHH7 pKa = 6.06SRR9 pKa = 11.84GHH11 pKa = 5.51GRR13 pKa = 11.84SNGILVLVTSTRR25 pKa = 11.84RR26 pKa = 11.84SAGTTASSGQNRR38 pKa = 11.84IEE40 pKa = 4.19SGRR43 pKa = 11.84LEE45 pKa = 4.34IGNVTLPCSRR55 pKa = 11.84RR56 pKa = 11.84HH57 pKa = 5.76SLLKK61 pKa = 10.21SHH63 pKa = 7.16LGISDD68 pKa = 3.28VRR70 pKa = 11.84IVLISRR76 pKa = 11.84LLTEE80 pKa = 4.17KK81 pKa = 10.25TSTSRR86 pKa = 11.84QCRR89 pKa = 11.84QSKK92 pKa = 10.05RR93 pKa = 11.84NPGHH97 pKa = 7.19KK98 pKa = 8.86ITSS101 pKa = 3.52

Molecular weight: 11.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1447

53

533

206.7

23.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.639 ± 1.638

1.313 ± 0.538

6.565 ± 0.859

3.594 ± 0.29

4.976 ± 0.816

6.289 ± 0.881

3.041 ± 0.505

4.147 ± 0.483

3.732 ± 0.763

6.98 ± 0.643

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.557 ± 0.277

4.492 ± 0.519

4.907 ± 0.661

4.077 ± 0.518

6.911 ± 1.011

9.122 ± 1.519

6.773 ± 1.203

6.634 ± 0.552

1.106 ± 0.328

4.147 ± 0.921
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski