Viral hemorrhagic septicemia virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Gammarhabdovirinae; Novirhabdovirus; Piscine novirhabdovirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4VAX6|S4VAX6_9RHAB GDP polyribonucleotidyltransferase OS=Viral hemorrhagic septicemia virus OX=11287 GN=L PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.39GGLRR6 pKa = 11.84AAFSGLNDD14 pKa = 3.32VRR16 pKa = 11.84IDD18 pKa = 3.5PTGGEE23 pKa = 4.17GRR25 pKa = 11.84VLVPGDD31 pKa = 3.53VEE33 pKa = 4.97LIVYY37 pKa = 9.72VGGFGEE43 pKa = 4.44EE44 pKa = 3.92DD45 pKa = 3.19RR46 pKa = 11.84KK47 pKa = 11.18VIVDD51 pKa = 3.84ALSALGGPQTVQALSVLLSYY71 pKa = 10.6VLQGNTQEE79 pKa = 5.28DD80 pKa = 5.29LEE82 pKa = 4.86TKK84 pKa = 10.55CKK86 pKa = 10.67VLTDD90 pKa = 3.37MGFKK94 pKa = 8.85VTQAVRR100 pKa = 11.84ATSIEE105 pKa = 4.05AGIMMPMRR113 pKa = 11.84EE114 pKa = 4.11LALTVNDD121 pKa = 4.5DD122 pKa = 3.6NLMEE126 pKa = 4.42IVKK129 pKa = 8.57GTLMTCSLLTKK140 pKa = 10.37YY141 pKa = 10.81SVDD144 pKa = 3.72KK145 pKa = 9.66MIKK148 pKa = 10.37YY149 pKa = 7.92ITKK152 pKa = 10.55KK153 pKa = 10.45LGEE156 pKa = 4.24LADD159 pKa = 3.93TQGVGEE165 pKa = 4.55LQHH168 pKa = 5.73FTADD172 pKa = 3.03KK173 pKa = 10.57AAIRR177 pKa = 11.84KK178 pKa = 8.49LAGCVRR184 pKa = 11.84PGQKK188 pKa = 7.96ITKK191 pKa = 9.34ALYY194 pKa = 10.43AFILTEE200 pKa = 3.84IADD203 pKa = 3.77PTTQSRR209 pKa = 11.84ARR211 pKa = 11.84AMGALRR217 pKa = 11.84LNGTGMTMIGLFTQAANNLGIAPAKK242 pKa = 10.36LLEE245 pKa = 4.44DD246 pKa = 3.75LCMEE250 pKa = 4.52SLVEE254 pKa = 3.87SARR257 pKa = 11.84RR258 pKa = 11.84IIQLMRR264 pKa = 11.84QVSEE268 pKa = 3.86AKK270 pKa = 10.34SIQEE274 pKa = 4.01RR275 pKa = 11.84YY276 pKa = 10.18AIMMSRR282 pKa = 11.84MLGEE286 pKa = 4.49SYY288 pKa = 10.77YY289 pKa = 11.0KK290 pKa = 10.58SYY292 pKa = 10.83GLNDD296 pKa = 3.4NSKK299 pKa = 9.96ISYY302 pKa = 9.71ILSQISGKK310 pKa = 10.03YY311 pKa = 9.9AVDD314 pKa = 3.4SLEE317 pKa = 3.87GLEE320 pKa = 4.64GIKK323 pKa = 9.22VTEE326 pKa = 4.2KK327 pKa = 10.21FRR329 pKa = 11.84EE330 pKa = 4.06FAEE333 pKa = 4.36LVAEE337 pKa = 4.19VLVDD341 pKa = 3.29KK342 pKa = 10.41YY343 pKa = 11.34EE344 pKa = 4.77RR345 pKa = 11.84IGEE348 pKa = 4.24DD349 pKa = 3.07STEE352 pKa = 3.94VSDD355 pKa = 5.0VIKK358 pKa = 10.46EE359 pKa = 3.92AARR362 pKa = 11.84QHH364 pKa = 5.75ARR366 pKa = 11.84RR367 pKa = 11.84TSAKK371 pKa = 9.81PEE373 pKa = 3.73PKK375 pKa = 10.08ARR377 pKa = 11.84NFRR380 pKa = 11.84SSTGRR385 pKa = 11.84GKK387 pKa = 9.68EE388 pKa = 3.93QEE390 pKa = 4.28TGEE393 pKa = 4.59SDD395 pKa = 4.29DD396 pKa = 5.12DD397 pKa = 5.31DD398 pKa = 4.73YY399 pKa = 12.03PEE401 pKa = 6.09DD402 pKa = 3.8SDD404 pKa = 4.34

Molecular weight: 44.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q08489|Q08489_9RHAB Non-virion protein OS=Viral hemorrhagic septicemia virus OX=11287 GN=NV PE=4 SV=1
MM1 pKa = 7.42TLFKK5 pKa = 10.38RR6 pKa = 11.84KK7 pKa = 8.44RR8 pKa = 11.84TILVPPPHH16 pKa = 6.44LTSNDD21 pKa = 3.32EE22 pKa = 4.34DD23 pKa = 3.71RR24 pKa = 11.84VSTILTEE31 pKa = 4.17GTLTITGPPPGNQVDD46 pKa = 4.38KK47 pKa = 11.47VCMAMKK53 pKa = 10.22LARR56 pKa = 11.84AILCEE61 pKa = 4.17DD62 pKa = 3.14QHH64 pKa = 6.64PAFNPLVHH72 pKa = 6.97LFQSAMIFGEE82 pKa = 4.23TSEE85 pKa = 5.33KK86 pKa = 10.04IDD88 pKa = 3.42FGTRR92 pKa = 11.84SKK94 pKa = 9.83TLITSFKK101 pKa = 9.8IAEE104 pKa = 4.22AKK106 pKa = 10.35AIYY109 pKa = 10.03LDD111 pKa = 3.81SSPVRR116 pKa = 11.84SRR118 pKa = 11.84IEE120 pKa = 3.74AKK122 pKa = 10.43KK123 pKa = 7.99YY124 pKa = 4.56TTPIRR129 pKa = 11.84HH130 pKa = 6.09GSVTYY135 pKa = 9.47YY136 pKa = 11.16GPFIFADD143 pKa = 3.49DD144 pKa = 3.77HH145 pKa = 7.49VGGKK149 pKa = 7.29GHH151 pKa = 6.61RR152 pKa = 11.84EE153 pKa = 3.6KK154 pKa = 11.12LGALCGFLQSEE165 pKa = 5.24PYY167 pKa = 10.24GQAKK171 pKa = 10.21DD172 pKa = 3.79YY173 pKa = 10.74YY174 pKa = 8.74NRR176 pKa = 11.84AVEE179 pKa = 4.22EE180 pKa = 4.47EE181 pKa = 4.0IGIPPRR187 pKa = 11.84DD188 pKa = 3.64PKK190 pKa = 10.69RR191 pKa = 11.84RR192 pKa = 11.84SGTSSVRR199 pKa = 11.84PWW201 pKa = 2.81

Molecular weight: 22.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3440

122

1984

573.3

64.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.337 ± 0.592

1.483 ± 0.357

5.349 ± 0.29

6.86 ± 0.815

3.372 ± 0.263

6.279 ± 0.461

2.645 ± 0.527

6.221 ± 0.215

6.483 ± 0.613

10.814 ± 1.003

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.279

3.052 ± 0.33

4.593 ± 0.752

3.866 ± 0.503

5.64 ± 0.553

7.645 ± 0.322

6.948 ± 0.572

5.901 ± 0.483

1.308 ± 0.418

2.733 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski