Bovine adenovirus 4 (BAdV-4)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Rowavirales; Adenoviridae; Atadenovirus; Bovine atadenovirus D

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q997H0|Q997H0_ADEB4 E4.2 OS=Bovine adenovirus 4 OX=70333 PE=4 SV=1
MM1 pKa = 7.0NQLSRR6 pKa = 11.84TFMGAAEE13 pKa = 4.2LVNYY17 pKa = 7.4FTDD20 pKa = 4.06LNSNQYY26 pKa = 11.07AIYY29 pKa = 10.25CVATSKK35 pKa = 11.09SLAYY39 pKa = 8.23WAKK42 pKa = 9.98GPFRR46 pKa = 11.84MGFRR50 pKa = 11.84FIGDD54 pKa = 3.46STVYY58 pKa = 10.96LLAQKK63 pKa = 10.73DD64 pKa = 3.97LFPEE68 pKa = 4.39FDD70 pKa = 4.16ALDD73 pKa = 3.72FLKK76 pKa = 10.23WANFSNMGMGFFAIEE91 pKa = 3.91WEE93 pKa = 4.11LLINSLVDD101 pKa = 3.67RR102 pKa = 11.84PMLTAEE108 pKa = 4.65VILIPGNTSHH118 pKa = 6.9EE119 pKa = 4.02SWEE122 pKa = 3.92FFKK125 pKa = 11.09YY126 pKa = 9.72YY127 pKa = 8.86WEE129 pKa = 4.33NYY131 pKa = 8.8FPYY134 pKa = 10.4FPIAPDD140 pKa = 3.33WEE142 pKa = 4.3

Molecular weight: 16.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q997I9|Q997I9_ADEB4 p32K OS=Bovine adenovirus 4 OX=70333 PE=4 SV=1
MM1 pKa = 7.27LTDD4 pKa = 4.43FYY6 pKa = 11.32NDD8 pKa = 3.48LMGGAYY14 pKa = 9.81RR15 pKa = 11.84RR16 pKa = 11.84TYY18 pKa = 10.69RR19 pKa = 11.84RR20 pKa = 11.84TRR22 pKa = 11.84KK23 pKa = 9.16AKK25 pKa = 9.52KK26 pKa = 9.67RR27 pKa = 11.84KK28 pKa = 9.06DD29 pKa = 3.3SRR31 pKa = 11.84RR32 pKa = 11.84SHH34 pKa = 6.62VNLKK38 pKa = 9.98KK39 pKa = 9.45RR40 pKa = 11.84TRR42 pKa = 11.84IARR45 pKa = 11.84NSNLYY50 pKa = 9.93SHH52 pKa = 7.08LRR54 pKa = 11.84LAPTAVNEE62 pKa = 4.16YY63 pKa = 9.23HH64 pKa = 6.53SKK66 pKa = 8.36PRR68 pKa = 11.84RR69 pKa = 11.84MSAVLHH75 pKa = 5.55MPISSVQLHH84 pKa = 5.63QIEE87 pKa = 4.58EE88 pKa = 4.38PKK90 pKa = 10.71HH91 pKa = 6.66SIASRR96 pKa = 11.84QFFNYY101 pKa = 9.06RR102 pKa = 11.84RR103 pKa = 11.84PPDD106 pKa = 3.67GEE108 pKa = 4.1EE109 pKa = 4.07DD110 pKa = 2.98VDD112 pKa = 3.89YY113 pKa = 10.85QAKK116 pKa = 10.3NKK118 pKa = 9.12WSLEE122 pKa = 3.96DD123 pKa = 3.52VISYY127 pKa = 8.68LQHH130 pKa = 6.12IPKK133 pKa = 9.77QVRR136 pKa = 11.84KK137 pKa = 10.33VILTSLFGATLGLIIDD153 pKa = 4.66ALLGGPWGLTTRR165 pKa = 11.84LLRR168 pKa = 11.84LIVSLVPGGKK178 pKa = 8.89ILLLALDD185 pKa = 3.77GLGYY189 pKa = 10.73FLGKK193 pKa = 10.2NNNPNLVAYY202 pKa = 10.13DD203 pKa = 3.57PDD205 pKa = 4.51LIKK208 pKa = 10.84FGTNIQRR215 pKa = 11.84NINGRR220 pKa = 11.84LTEE223 pKa = 4.93DD224 pKa = 2.81IVRR227 pKa = 11.84AAEE230 pKa = 3.94EE231 pKa = 3.87QLGGGFMRR239 pKa = 11.84TLAALLSAAASAGTHH254 pKa = 6.28LKK256 pKa = 10.0IALPAIPLAVIKK268 pKa = 9.89PFQRR272 pKa = 4.11

Molecular weight: 30.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

10427

54

1073

289.6

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.217 ± 0.292

2.58 ± 0.449

4.555 ± 0.216

5.63 ± 0.349

5.495 ± 0.226

4.555 ± 0.306

2.378 ± 0.214

5.965 ± 0.225

6.445 ± 0.613

10.674 ± 0.525

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.052 ± 0.171

6.272 ± 0.313

5.304 ± 0.259

4.066 ± 0.269

4.853 ± 0.386

7.413 ± 0.327

5.505 ± 0.329

5.265 ± 0.32

1.698 ± 0.194

4.076 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski