Hubei tombus-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGL0|A0A1L3KGL0_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 5 OX=1923292 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.26SVAPDD7 pKa = 3.46HH8 pKa = 7.56PDD10 pKa = 3.0LQVRR14 pKa = 11.84KK15 pKa = 9.32RR16 pKa = 11.84WGDD19 pKa = 3.28TKK21 pKa = 10.64VRR23 pKa = 11.84KK24 pKa = 6.48TARR27 pKa = 11.84VSGISPRR34 pKa = 11.84MEE36 pKa = 3.5LKK38 pKa = 10.78SFNNDD43 pKa = 1.84IDD45 pKa = 3.97TLEE48 pKa = 4.21RR49 pKa = 11.84AVKK52 pKa = 10.09EE53 pKa = 3.55RR54 pKa = 11.84VFFVKK59 pKa = 10.48NDD61 pKa = 3.27LGEE64 pKa = 4.22FVEE67 pKa = 4.87PPKK70 pKa = 10.08PAPHH74 pKa = 6.96HH75 pKa = 5.69FRR77 pKa = 11.84KK78 pKa = 10.43AMLATEE84 pKa = 4.11KK85 pKa = 10.92LLVRR89 pKa = 11.84RR90 pKa = 11.84LPKK93 pKa = 9.48TAPLSRR99 pKa = 11.84DD100 pKa = 3.05AFVEE104 pKa = 4.07TFRR107 pKa = 11.84GRR109 pKa = 11.84KK110 pKa = 8.6RR111 pKa = 11.84EE112 pKa = 3.9IYY114 pKa = 10.09AKK116 pKa = 10.2AVEE119 pKa = 4.45SLSSQSVTQRR129 pKa = 11.84DD130 pKa = 3.11AGVQVFVKK138 pKa = 10.42FEE140 pKa = 3.92KK141 pKa = 10.02TDD143 pKa = 3.46YY144 pKa = 9.79TRR146 pKa = 11.84KK147 pKa = 9.53KK148 pKa = 10.49DD149 pKa = 3.43PVPRR153 pKa = 11.84VISPRR158 pKa = 11.84NPRR161 pKa = 11.84YY162 pKa = 8.55NVEE165 pKa = 3.58VGRR168 pKa = 11.84YY169 pKa = 8.5LRR171 pKa = 11.84TIEE174 pKa = 4.26EE175 pKa = 4.41PLFHH179 pKa = 7.06SLSQLFEE186 pKa = 4.11GKK188 pKa = 8.43RR189 pKa = 11.84TVFKK193 pKa = 10.91GMNAADD199 pKa = 4.42SGRR202 pKa = 11.84AMYY205 pKa = 10.08DD206 pKa = 2.66LWSSFRR212 pKa = 11.84KK213 pKa = 9.13PVAVGLDD220 pKa = 3.18ASRR223 pKa = 11.84FDD225 pKa = 3.56QHH227 pKa = 6.64VSKK230 pKa = 10.97CALQWEE236 pKa = 4.6HH237 pKa = 7.14AIYY240 pKa = 8.55PQCFTSSGDD249 pKa = 3.33RR250 pKa = 11.84AEE252 pKa = 4.61LRR254 pKa = 11.84RR255 pKa = 11.84LLKK258 pKa = 9.72WQVHH262 pKa = 5.09NKK264 pKa = 9.46CVGYY268 pKa = 10.33CADD271 pKa = 3.94GRR273 pKa = 11.84IKK275 pKa = 9.36YY276 pKa = 7.66TKK278 pKa = 9.85EE279 pKa = 3.56GTRR282 pKa = 11.84MSGDD286 pKa = 3.41MNTSLGNCVLMCSMIKK302 pKa = 10.05QYY304 pKa = 11.37SLDD307 pKa = 3.57RR308 pKa = 11.84GVRR311 pKa = 11.84TLLANNGDD319 pKa = 3.82DD320 pKa = 3.43CVVFMEE326 pKa = 4.63ADD328 pKa = 3.23EE329 pKa = 4.4LARR332 pKa = 11.84FSSGLDD338 pKa = 2.85EE339 pKa = 4.68WFRR342 pKa = 11.84AMGFNMVVEE351 pKa = 4.57PPCYY355 pKa = 9.21QFEE358 pKa = 4.42EE359 pKa = 4.53IEE361 pKa = 4.43FCQTHH366 pKa = 7.26PIYY369 pKa = 10.74VGPKK373 pKa = 8.16HH374 pKa = 6.94SDD376 pKa = 3.25YY377 pKa = 11.69LMVRR381 pKa = 11.84HH382 pKa = 6.27PKK384 pKa = 8.62WALAKK389 pKa = 10.66DD390 pKa = 3.77SMCIHH395 pKa = 7.12GFPTSKK401 pKa = 9.78IYY403 pKa = 10.36KK404 pKa = 8.74AWLDD408 pKa = 3.6AVGTGGLAMTGGVPIFQDD426 pKa = 5.37FYY428 pKa = 11.37STYY431 pKa = 9.9CKK433 pKa = 10.56YY434 pKa = 10.33GVRR437 pKa = 11.84GKK439 pKa = 7.3THH441 pKa = 6.07FHH443 pKa = 5.02EE444 pKa = 4.59QSWGVRR450 pKa = 11.84SLQQGMVRR458 pKa = 11.84KK459 pKa = 9.41YY460 pKa = 10.73GAVLPEE466 pKa = 4.21TRR468 pKa = 11.84ASFYY472 pKa = 10.04WAFGVLPDD480 pKa = 3.91EE481 pKa = 4.27QLAIEE486 pKa = 4.25DD487 pKa = 4.68FYY489 pKa = 11.64RR490 pKa = 11.84GVKK493 pKa = 9.97LDD495 pKa = 3.7EE496 pKa = 4.14AWRR499 pKa = 11.84DD500 pKa = 3.51EE501 pKa = 5.94LEE503 pKa = 4.25FQPLLPLL510 pKa = 4.0

Molecular weight: 58.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGL0|A0A1L3KGL0_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 5 OX=1923292 PE=4 SV=1
MM1 pKa = 6.59VTIILEE7 pKa = 4.09AALISSVIVPLATTTALRR25 pKa = 11.84VSRR28 pKa = 11.84WMEE31 pKa = 3.52RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NALAKK40 pKa = 9.92EE41 pKa = 4.23CIARR45 pKa = 11.84FDD47 pKa = 3.9NLDD50 pKa = 3.38GSFANVLEE58 pKa = 4.47SARR61 pKa = 11.84QDD63 pKa = 3.31SARR66 pKa = 11.84RR67 pKa = 11.84DD68 pKa = 3.91AIEE71 pKa = 4.08ATAVTSAVLSNMGVVGVTVEE91 pKa = 4.19SACAQKK97 pKa = 10.49AAEE100 pKa = 4.23RR101 pKa = 11.84MRR103 pKa = 11.84EE104 pKa = 3.65QRR106 pKa = 11.84GWIRR110 pKa = 11.84IRR112 pKa = 11.84NHH114 pKa = 6.76RR115 pKa = 11.84LTEE118 pKa = 4.08LAFSAADD125 pKa = 3.32EE126 pKa = 4.86AYY128 pKa = 10.68NEE130 pKa = 4.08FGARR134 pKa = 11.84SLSKK138 pKa = 11.17ANDD141 pKa = 3.17LVTRR145 pKa = 11.84KK146 pKa = 9.4FLRR149 pKa = 11.84DD150 pKa = 3.53FFRR153 pKa = 11.84EE154 pKa = 4.25CKK156 pKa = 10.18DD157 pKa = 4.53LRR159 pKa = 11.84TKK161 pKa = 10.45DD162 pKa = 3.24ANRR165 pKa = 11.84AIEE168 pKa = 3.83IALPFSYY175 pKa = 10.38LPPAEE180 pKa = 4.28RR181 pKa = 11.84GSMQEE186 pKa = 4.86FVTLDD191 pKa = 3.45PFVEE195 pKa = 4.39RR196 pKa = 11.84VGPADD201 pKa = 4.86LRR203 pKa = 11.84VQQ205 pKa = 3.46

Molecular weight: 22.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

715

205

510

357.5

40.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.811 ± 2.78

1.958 ± 0.258

5.734 ± 0.192

6.573 ± 0.642

5.455 ± 0.3

5.734 ± 1.208

1.958 ± 0.766

3.357 ± 0.793

5.734 ± 1.463

8.112 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.937 ± 0.259

2.797 ± 0.576

4.615 ± 0.88

3.217 ± 0.405

9.231 ± 1.544

6.434 ± 0.206

4.755 ± 0.318

7.972 ± 0.087

1.678 ± 0.366

2.937 ± 1.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski