Arthrobacter phage Temper16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Korravirus; unclassified Korravirus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XT60|A0A249XT60_9CAUD HNH endonuclease OS=Arthrobacter phage Temper16 OX=2027887 GN=TEMPER16_60 PE=4 SV=1
MM1 pKa = 7.68INEE4 pKa = 4.75AAPALIEE11 pKa = 3.84QLNEE15 pKa = 3.53VGAEE19 pKa = 3.88LEE21 pKa = 4.6RR22 pKa = 11.84IHH24 pKa = 6.37EE25 pKa = 4.65AEE27 pKa = 4.19GQEE30 pKa = 4.16PPLTTGAIFLNVRR43 pKa = 11.84TGRR46 pKa = 11.84VWFQIQDD53 pKa = 3.32QSYY56 pKa = 10.6GSTLWVSKK64 pKa = 10.22HH65 pKa = 5.32EE66 pKa = 4.05RR67 pKa = 11.84HH68 pKa = 5.94AAPGEE73 pKa = 4.13TLVSVEE79 pKa = 4.32SMEE82 pKa = 4.33EE83 pKa = 3.99AINYY87 pKa = 7.6EE88 pKa = 3.6WDD90 pKa = 3.05

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XT40|A0A249XT40_9CAUD Uncharacterized protein OS=Arthrobacter phage Temper16 OX=2027887 GN=TEMPER16_40 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 4.95RR3 pKa = 11.84RR4 pKa = 11.84NYY6 pKa = 9.74NHH8 pKa = 7.12PTSQRR13 pKa = 11.84IPRR16 pKa = 11.84KK17 pKa = 9.61GKK19 pKa = 7.65EE20 pKa = 3.99LKK22 pKa = 10.75GGAKK26 pKa = 10.21SIRR29 pKa = 11.84FTADD33 pKa = 2.2YY34 pKa = 8.73WAALRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84KK42 pKa = 9.78QEE44 pKa = 3.67AQSRR48 pKa = 11.84LLQRR52 pKa = 11.84QVRR55 pKa = 11.84DD56 pKa = 3.14HH57 pKa = 6.86LRR59 pKa = 11.84FLRR62 pKa = 11.84EE63 pKa = 3.76DD64 pKa = 3.88DD65 pKa = 4.02GRR67 pKa = 11.84QPMYY71 pKa = 11.28VFFGSGKK78 pKa = 9.28EE79 pKa = 3.81AEE81 pKa = 4.08LWSRR85 pKa = 11.84EE86 pKa = 3.76FMLALLEE93 pKa = 4.38VGEE96 pKa = 4.34VWDD99 pKa = 4.54PRR101 pKa = 11.84KK102 pKa = 10.51QMIQARR108 pKa = 11.84IEE110 pKa = 3.88RR111 pKa = 11.84MRR113 pKa = 11.84GHH115 pKa = 7.54RR116 pKa = 11.84ARR118 pKa = 11.84LVAVWSSDD126 pKa = 3.33PMPTSDD132 pKa = 3.95YY133 pKa = 10.57RR134 pKa = 11.84YY135 pKa = 8.16QQEE138 pKa = 4.24VDD140 pKa = 2.84TMYY143 pKa = 10.91AIEE146 pKa = 4.91DD147 pKa = 3.69LNRR150 pKa = 11.84INGYY154 pKa = 7.96EE155 pKa = 4.05VSRR158 pKa = 11.84EE159 pKa = 3.99TASVV163 pKa = 3.04

Molecular weight: 19.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

14027

39

877

233.8

25.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.18 ± 0.327

0.727 ± 0.137

5.739 ± 0.197

6.402 ± 0.343

3.016 ± 0.216

8.833 ± 0.417

1.861 ± 0.17

4.755 ± 0.307

4.876 ± 0.298

8.477 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.31 ± 0.134

3.629 ± 0.195

4.976 ± 0.235

4.007 ± 0.175

5.796 ± 0.327

6.11 ± 0.291

6.459 ± 0.385

7.257 ± 0.253

2.032 ± 0.205

2.559 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski