Amino acid dipepetide frequency for Arthrobacter phage Temper16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.12AlaAla: 12.12 ± 1.125
0.713AlaCys: 0.713 ± 0.278
5.419AlaAsp: 5.419 ± 0.5
6.844AlaGlu: 6.844 ± 0.769
2.567AlaPhe: 2.567 ± 0.476
8.342AlaGly: 8.342 ± 1.064
1.069AlaHis: 1.069 ± 0.292
5.419AlaIle: 5.419 ± 0.744
5.775AlaLys: 5.775 ± 0.847
8.841AlaLeu: 8.841 ± 1.301
2.567AlaMet: 2.567 ± 0.561
3.779AlaAsn: 3.779 ± 0.438
4.42AlaPro: 4.42 ± 0.615
4.42AlaGln: 4.42 ± 0.643
5.846AlaArg: 5.846 ± 0.749
6.417AlaSer: 6.417 ± 0.587
6.345AlaThr: 6.345 ± 0.713
5.989AlaVal: 5.989 ± 0.75
1.925AlaTrp: 1.925 ± 0.411
2.994AlaTyr: 2.994 ± 0.544
0.0AlaXaa: 0.0 ± 0.0
Cys
0.428CysAla: 0.428 ± 0.201
0.0CysCys: 0.0 ± 0.0
0.499CysAsp: 0.499 ± 0.227
0.57CysGlu: 0.57 ± 0.261
0.214CysPhe: 0.214 ± 0.114
1.212CysGly: 1.212 ± 0.349
0.214CysHis: 0.214 ± 0.105
0.428CysIle: 0.428 ± 0.167
0.285CysLys: 0.285 ± 0.125
0.356CysLeu: 0.356 ± 0.148
0.0CysMet: 0.0 ± 0.0
0.143CysAsn: 0.143 ± 0.101
0.356CysPro: 0.356 ± 0.173
0.499CysGln: 0.499 ± 0.188
0.499CysArg: 0.499 ± 0.22
0.356CysSer: 0.356 ± 0.156
0.428CysThr: 0.428 ± 0.209
0.214CysVal: 0.214 ± 0.128
0.356CysTrp: 0.356 ± 0.169
0.214CysTyr: 0.214 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
6.06AspAla: 6.06 ± 0.542
0.57AspCys: 0.57 ± 0.199
3.28AspAsp: 3.28 ± 0.48
4.919AspGlu: 4.919 ± 0.801
1.854AspPhe: 1.854 ± 0.417
5.276AspGly: 5.276 ± 0.748
1.069AspHis: 1.069 ± 0.432
2.21AspIle: 2.21 ± 0.348
1.996AspLys: 1.996 ± 0.361
5.704AspLeu: 5.704 ± 0.625
1.283AspMet: 1.283 ± 0.277
1.355AspAsn: 1.355 ± 0.312
3.636AspPro: 3.636 ± 0.487
2.281AspGln: 2.281 ± 0.487
3.707AspArg: 3.707 ± 0.538
3.208AspSer: 3.208 ± 0.476
3.422AspThr: 3.422 ± 0.583
2.638AspVal: 2.638 ± 0.355
1.141AspTrp: 1.141 ± 0.302
1.782AspTyr: 1.782 ± 0.373
0.0AspXaa: 0.0 ± 0.0
Glu
6.987GluAla: 6.987 ± 0.952
0.642GluCys: 0.642 ± 0.223
3.993GluAsp: 3.993 ± 0.803
4.064GluGlu: 4.064 ± 0.621
2.353GluPhe: 2.353 ± 0.423
6.417GluGly: 6.417 ± 0.594
1.283GluHis: 1.283 ± 0.31
2.424GluIle: 2.424 ± 0.458
3.066GluLys: 3.066 ± 0.512
5.918GluLeu: 5.918 ± 0.616
1.426GluMet: 1.426 ± 0.404
2.068GluAsn: 2.068 ± 0.351
2.567GluPro: 2.567 ± 0.473
3.208GluGln: 3.208 ± 0.52
4.206GluArg: 4.206 ± 0.616
2.781GluSer: 2.781 ± 0.397
3.565GluThr: 3.565 ± 0.564
4.42GluVal: 4.42 ± 0.704
1.426GluTrp: 1.426 ± 0.351
1.212GluTyr: 1.212 ± 0.314
0.0GluXaa: 0.0 ± 0.0
Phe
2.852PheAla: 2.852 ± 0.602
0.143PheCys: 0.143 ± 0.092
2.567PheAsp: 2.567 ± 0.401
2.068PheGlu: 2.068 ± 0.414
1.569PhePhe: 1.569 ± 0.545
3.137PheGly: 3.137 ± 0.596
0.642PheHis: 0.642 ± 0.2
0.998PheIle: 0.998 ± 0.343
1.497PheLys: 1.497 ± 0.297
1.711PheLeu: 1.711 ± 0.274
0.856PheMet: 0.856 ± 0.318
1.283PheAsn: 1.283 ± 0.33
0.57PhePro: 0.57 ± 0.202
1.355PheGln: 1.355 ± 0.36
2.21PheArg: 2.21 ± 0.346
1.854PheSer: 1.854 ± 0.667
2.068PheThr: 2.068 ± 0.546
1.782PheVal: 1.782 ± 0.413
0.214PheTrp: 0.214 ± 0.121
0.784PheTyr: 0.784 ± 0.358
0.0PheXaa: 0.0 ± 0.0
Gly
8.342GlyAla: 8.342 ± 1.152
0.642GlyCys: 0.642 ± 0.217
4.492GlyAsp: 4.492 ± 0.684
5.632GlyGlu: 5.632 ± 0.681
3.208GlyPhe: 3.208 ± 0.66
5.918GlyGly: 5.918 ± 0.543
2.139GlyHis: 2.139 ± 0.529
4.349GlyIle: 4.349 ± 0.691
3.636GlyLys: 3.636 ± 0.384
7.201GlyLeu: 7.201 ± 0.864
2.068GlyMet: 2.068 ± 0.359
4.135GlyAsn: 4.135 ± 0.889
4.064GlyPro: 4.064 ± 0.512
3.565GlyGln: 3.565 ± 0.45
4.919GlyArg: 4.919 ± 0.522
6.488GlySer: 6.488 ± 0.966
5.989GlyThr: 5.989 ± 0.991
7.486GlyVal: 7.486 ± 0.821
1.782GlyTrp: 1.782 ± 0.379
2.281GlyTyr: 2.281 ± 0.451
0.0GlyXaa: 0.0 ± 0.0
His
1.854HisAla: 1.854 ± 0.416
0.214HisCys: 0.214 ± 0.112
0.856HisAsp: 0.856 ± 0.227
0.784HisGlu: 0.784 ± 0.264
0.642HisPhe: 0.642 ± 0.201
1.925HisGly: 1.925 ± 0.401
0.499HisHis: 0.499 ± 0.211
1.355HisIle: 1.355 ± 0.318
0.856HisLys: 0.856 ± 0.224
1.782HisLeu: 1.782 ± 0.448
0.499HisMet: 0.499 ± 0.185
0.856HisAsn: 0.856 ± 0.274
0.713HisPro: 0.713 ± 0.26
0.57HisGln: 0.57 ± 0.205
0.642HisArg: 0.642 ± 0.196
1.069HisSer: 1.069 ± 0.255
1.141HisThr: 1.141 ± 0.336
0.784HisVal: 0.784 ± 0.261
0.428HisTrp: 0.428 ± 0.182
1.141HisTyr: 1.141 ± 0.34
0.0HisXaa: 0.0 ± 0.0
Ile
4.706IleAla: 4.706 ± 0.596
0.214IleCys: 0.214 ± 0.146
2.852IleAsp: 2.852 ± 0.472
2.852IleGlu: 2.852 ± 0.435
1.141IlePhe: 1.141 ± 0.36
3.85IleGly: 3.85 ± 0.733
0.856IleHis: 0.856 ± 0.229
1.996IleIle: 1.996 ± 0.582
2.638IleLys: 2.638 ± 0.461
3.636IleLeu: 3.636 ± 0.541
0.927IleMet: 0.927 ± 0.367
2.21IleAsn: 2.21 ± 0.404
1.925IlePro: 1.925 ± 0.473
2.781IleGln: 2.781 ± 0.373
3.422IleArg: 3.422 ± 0.478
3.707IleSer: 3.707 ± 0.67
2.994IleThr: 2.994 ± 0.536
3.28IleVal: 3.28 ± 0.611
0.642IleTrp: 0.642 ± 0.167
0.927IleTyr: 0.927 ± 0.211
0.0IleXaa: 0.0 ± 0.0
Lys
5.846LysAla: 5.846 ± 0.745
0.57LysCys: 0.57 ± 0.184
2.781LysAsp: 2.781 ± 0.486
2.709LysGlu: 2.709 ± 0.483
1.711LysPhe: 1.711 ± 0.416
4.064LysGly: 4.064 ± 0.557
1.426LysHis: 1.426 ± 0.357
2.638LysIle: 2.638 ± 0.451
2.068LysLys: 2.068 ± 0.424
4.706LysLeu: 4.706 ± 0.626
1.355LysMet: 1.355 ± 0.294
0.713LysAsn: 0.713 ± 0.245
2.781LysPro: 2.781 ± 0.524
1.569LysGln: 1.569 ± 0.315
2.495LysArg: 2.495 ± 0.442
2.709LysSer: 2.709 ± 0.484
2.923LysThr: 2.923 ± 0.457
3.993LysVal: 3.993 ± 0.669
0.499LysTrp: 0.499 ± 0.185
1.212LysTyr: 1.212 ± 0.235
0.0LysXaa: 0.0 ± 0.0
Leu
8.983LeuAla: 8.983 ± 0.943
0.642LeuCys: 0.642 ± 0.24
5.704LeuAsp: 5.704 ± 0.44
4.064LeuGlu: 4.064 ± 0.612
2.424LeuPhe: 2.424 ± 0.522
8.27LeuGly: 8.27 ± 1.041
1.497LeuHis: 1.497 ± 0.361
4.206LeuIle: 4.206 ± 0.479
2.923LeuLys: 2.923 ± 0.475
6.987LeuLeu: 6.987 ± 0.993
1.782LeuMet: 1.782 ± 0.401
3.707LeuAsn: 3.707 ± 0.487
5.062LeuPro: 5.062 ± 0.755
3.351LeuGln: 3.351 ± 0.391
5.775LeuArg: 5.775 ± 0.626
4.206LeuSer: 4.206 ± 0.566
5.276LeuThr: 5.276 ± 0.433
6.417LeuVal: 6.417 ± 0.634
1.497LeuTrp: 1.497 ± 0.292
1.996LeuTyr: 1.996 ± 0.342
0.0LeuXaa: 0.0 ± 0.0
Met
2.781MetAla: 2.781 ± 0.472
0.071MetCys: 0.071 ± 0.083
1.069MetAsp: 1.069 ± 0.344
1.711MetGlu: 1.711 ± 0.357
0.285MetPhe: 0.285 ± 0.147
1.497MetGly: 1.497 ± 0.369
0.285MetHis: 0.285 ± 0.12
1.782MetIle: 1.782 ± 0.377
1.212MetLys: 1.212 ± 0.301
1.212MetLeu: 1.212 ± 0.21
0.356MetMet: 0.356 ± 0.13
0.998MetAsn: 0.998 ± 0.236
1.854MetPro: 1.854 ± 0.41
0.499MetGln: 0.499 ± 0.256
0.642MetArg: 0.642 ± 0.244
1.782MetSer: 1.782 ± 0.348
1.64MetThr: 1.64 ± 0.375
2.495MetVal: 2.495 ± 0.423
0.356MetTrp: 0.356 ± 0.186
0.57MetTyr: 0.57 ± 0.241
0.0MetXaa: 0.0 ± 0.0
Asn
2.852AsnAla: 2.852 ± 0.353
0.356AsnCys: 0.356 ± 0.163
1.711AsnAsp: 1.711 ± 0.398
1.711AsnGlu: 1.711 ± 0.306
0.927AsnPhe: 0.927 ± 0.343
4.135AsnGly: 4.135 ± 0.674
0.856AsnHis: 0.856 ± 0.375
1.355AsnIle: 1.355 ± 0.285
1.854AsnLys: 1.854 ± 0.364
3.208AsnLeu: 3.208 ± 0.431
0.499AsnMet: 0.499 ± 0.206
1.355AsnAsn: 1.355 ± 0.5
1.996AsnPro: 1.996 ± 0.394
1.854AsnGln: 1.854 ± 0.35
1.782AsnArg: 1.782 ± 0.433
1.782AsnSer: 1.782 ± 0.375
2.852AsnThr: 2.852 ± 0.413
3.066AsnVal: 3.066 ± 0.524
1.069AsnTrp: 1.069 ± 0.283
1.069AsnTyr: 1.069 ± 0.294
0.0AsnXaa: 0.0 ± 0.0
Pro
5.49ProAla: 5.49 ± 0.571
0.428ProCys: 0.428 ± 0.188
2.923ProAsp: 2.923 ± 0.51
3.28ProGlu: 3.28 ± 0.564
1.069ProPhe: 1.069 ± 0.294
4.848ProGly: 4.848 ± 0.573
0.499ProHis: 0.499 ± 0.23
2.139ProIle: 2.139 ± 0.458
3.066ProLys: 3.066 ± 0.528
3.066ProLeu: 3.066 ± 0.479
1.283ProMet: 1.283 ± 0.319
1.854ProAsn: 1.854 ± 0.351
1.569ProPro: 1.569 ± 0.313
1.069ProGln: 1.069 ± 0.24
2.353ProArg: 2.353 ± 0.425
3.921ProSer: 3.921 ± 0.619
3.779ProThr: 3.779 ± 0.716
5.062ProVal: 5.062 ± 0.673
1.497ProTrp: 1.497 ± 0.386
0.57ProTyr: 0.57 ± 0.228
0.0ProXaa: 0.0 ± 0.0
Gln
3.565GlnAla: 3.565 ± 0.489
0.143GlnCys: 0.143 ± 0.103
2.21GlnAsp: 2.21 ± 0.442
2.424GlnGlu: 2.424 ± 0.377
1.283GlnPhe: 1.283 ± 0.304
2.852GlnGly: 2.852 ± 0.538
0.499GlnHis: 0.499 ± 0.152
1.854GlnIle: 1.854 ± 0.349
2.281GlnLys: 2.281 ± 0.332
5.062GlnLeu: 5.062 ± 0.717
1.212GlnMet: 1.212 ± 0.253
1.64GlnAsn: 1.64 ± 0.372
2.139GlnPro: 2.139 ± 0.428
1.925GlnGln: 1.925 ± 0.508
1.996GlnArg: 1.996 ± 0.409
1.569GlnSer: 1.569 ± 0.369
2.139GlnThr: 2.139 ± 0.319
3.422GlnVal: 3.422 ± 0.421
0.998GlnTrp: 0.998 ± 0.24
0.856GlnTyr: 0.856 ± 0.259
0.0GlnXaa: 0.0 ± 0.0
Arg
5.704ArgAla: 5.704 ± 0.675
0.499ArgCys: 0.499 ± 0.2
3.636ArgAsp: 3.636 ± 0.618
3.636ArgGlu: 3.636 ± 0.638
1.711ArgPhe: 1.711 ± 0.354
3.707ArgGly: 3.707 ± 0.469
1.141ArgHis: 1.141 ± 0.329
3.208ArgIle: 3.208 ± 0.519
4.064ArgLys: 4.064 ± 0.638
5.704ArgLeu: 5.704 ± 0.738
1.426ArgMet: 1.426 ± 0.347
2.353ArgAsn: 2.353 ± 0.41
2.781ArgPro: 2.781 ± 0.485
2.353ArgGln: 2.353 ± 0.426
3.707ArgArg: 3.707 ± 0.569
3.066ArgSer: 3.066 ± 0.391
3.066ArgThr: 3.066 ± 0.414
3.779ArgVal: 3.779 ± 0.567
0.998ArgTrp: 0.998 ± 0.351
1.426ArgTyr: 1.426 ± 0.392
0.0ArgXaa: 0.0 ± 0.0
Ser
4.349SerAla: 4.349 ± 0.672
0.428SerCys: 0.428 ± 0.211
3.208SerAsp: 3.208 ± 0.508
4.777SerGlu: 4.777 ± 0.47
1.64SerPhe: 1.64 ± 0.336
8.056SerGly: 8.056 ± 1.088
1.141SerHis: 1.141 ± 0.263
3.494SerIle: 3.494 ± 0.559
2.353SerLys: 2.353 ± 0.356
4.991SerLeu: 4.991 ± 0.779
1.497SerMet: 1.497 ± 0.499
1.64SerAsn: 1.64 ± 0.324
3.137SerPro: 3.137 ± 0.507
1.64SerGln: 1.64 ± 0.344
2.923SerArg: 2.923 ± 0.421
3.422SerSer: 3.422 ± 0.535
4.278SerThr: 4.278 ± 0.674
4.777SerVal: 4.777 ± 0.572
1.069SerTrp: 1.069 ± 0.419
2.281SerTyr: 2.281 ± 0.405
0.0SerXaa: 0.0 ± 0.0
Thr
7.415ThrAla: 7.415 ± 0.918
0.356ThrCys: 0.356 ± 0.155
3.351ThrAsp: 3.351 ± 0.508
4.42ThrGlu: 4.42 ± 0.515
1.996ThrPhe: 1.996 ± 0.475
5.205ThrGly: 5.205 ± 1.099
0.927ThrHis: 0.927 ± 0.215
2.353ThrIle: 2.353 ± 0.484
3.066ThrLys: 3.066 ± 0.496
4.991ThrLeu: 4.991 ± 0.572
1.212ThrMet: 1.212 ± 0.321
2.139ThrAsn: 2.139 ± 0.388
3.707ThrPro: 3.707 ± 0.505
2.424ThrGln: 2.424 ± 0.452
3.779ThrArg: 3.779 ± 0.534
4.706ThrSer: 4.706 ± 0.565
5.419ThrThr: 5.419 ± 0.87
4.42ThrVal: 4.42 ± 0.565
0.927ThrTrp: 0.927 ± 0.332
1.782ThrTyr: 1.782 ± 0.436
0.0ThrXaa: 0.0 ± 0.0
Val
6.488ValAla: 6.488 ± 0.66
0.285ValCys: 0.285 ± 0.134
3.85ValAsp: 3.85 ± 0.561
4.848ValGlu: 4.848 ± 0.517
1.996ValPhe: 1.996 ± 0.588
5.704ValGly: 5.704 ± 0.847
1.569ValHis: 1.569 ± 0.295
3.565ValIle: 3.565 ± 0.629
3.85ValLys: 3.85 ± 0.467
5.918ValLeu: 5.918 ± 0.733
2.21ValMet: 2.21 ± 0.347
2.709ValAsn: 2.709 ± 0.434
4.278ValPro: 4.278 ± 0.454
3.565ValGln: 3.565 ± 0.524
3.921ValArg: 3.921 ± 0.451
5.205ValSer: 5.205 ± 0.556
3.993ValThr: 3.993 ± 0.48
4.991ValVal: 4.991 ± 0.543
1.925ValTrp: 1.925 ± 0.345
1.711ValTyr: 1.711 ± 0.334
0.0ValXaa: 0.0 ± 0.0
Trp
2.709TrpAla: 2.709 ± 0.362
0.071TrpCys: 0.071 ± 0.079
1.355TrpAsp: 1.355 ± 0.417
0.998TrpGlu: 0.998 ± 0.347
0.784TrpPhe: 0.784 ± 0.238
1.426TrpGly: 1.426 ± 0.356
0.356TrpHis: 0.356 ± 0.177
0.856TrpIle: 0.856 ± 0.246
0.998TrpLys: 0.998 ± 0.281
1.854TrpLeu: 1.854 ± 0.317
0.356TrpMet: 0.356 ± 0.161
0.642TrpAsn: 0.642 ± 0.213
0.784TrpPro: 0.784 ± 0.303
0.428TrpGln: 0.428 ± 0.194
0.998TrpArg: 0.998 ± 0.28
1.569TrpSer: 1.569 ± 0.365
1.141TrpThr: 1.141 ± 0.306
1.925TrpVal: 1.925 ± 0.326
0.713TrpTrp: 0.713 ± 0.194
0.285TrpTyr: 0.285 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.281TyrAla: 2.281 ± 0.39
0.285TyrCys: 0.285 ± 0.152
1.64TyrAsp: 1.64 ± 0.306
1.711TyrGlu: 1.711 ± 0.301
0.784TyrPhe: 0.784 ± 0.241
2.495TyrGly: 2.495 ± 0.47
0.642TyrHis: 0.642 ± 0.177
0.927TyrIle: 0.927 ± 0.241
1.283TyrLys: 1.283 ± 0.329
1.925TyrLeu: 1.925 ± 0.358
0.214TyrMet: 0.214 ± 0.124
0.713TyrAsn: 0.713 ± 0.244
1.426TyrPro: 1.426 ± 0.326
0.713TyrGln: 0.713 ± 0.259
2.139TyrArg: 2.139 ± 0.305
1.283TyrSer: 1.283 ± 0.386
2.139TyrThr: 2.139 ± 0.447
1.64TyrVal: 1.64 ± 0.276
0.856TyrTrp: 0.856 ± 0.247
0.499TyrTyr: 0.499 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (14027 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski