Escherichia phage LL11
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385IPI7|A0A385IPI7_9CAUD DNA-directed DNA polymerase OS=Escherichia phage LL11 OX=2315628 GN=CPT_LL11_018 PE=3 SV=1
MM1 pKa = 7.87 DD2 pKa = 5.65 RR3 pKa = 11.84 LDD5 pKa = 3.28 IVYY8 pKa = 10.48 LFFTFFLALLMLACYY23 pKa = 10.59 GSMRR27 pKa = 11.84 LDD29 pKa = 3.32 IPDD32 pKa = 3.71 EE33 pKa = 4.29 EE34 pKa = 4.51 EE35 pKa = 5.35 GYY37 pKa = 10.68 DD38 pKa = 3.39
Molecular weight: 4.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.804
IPC2_protein 3.77
IPC_protein 3.605
Toseland 3.427
ProMoST 3.694
Dawson 3.63
Bjellqvist 3.923
Wikipedia 3.617
Rodwell 3.465
Grimsley 3.35
Solomon 3.567
Lehninger 3.528
Nozaki 3.795
DTASelect 3.961
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.745
Patrickios 0.477
IPC_peptide 3.567
IPC2_peptide 3.706
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A385IQA0|A0A385IQA0_9CAUD DNA primase/helicase OS=Escherichia phage LL11 OX=2315628 GN=CPT_LL11_013 PE=4 SV=1
MM1 pKa = 7.45 EE2 pKa = 4.63 IWRR5 pKa = 11.84 PIAGIPDD12 pKa = 3.65 YY13 pKa = 9.98 MVSSFGRR20 pKa = 11.84 VRR22 pKa = 11.84 SHH24 pKa = 6.46 KK25 pKa = 9.75 RR26 pKa = 11.84 GKK28 pKa = 8.66 VTILNPPHH36 pKa = 7.39 DD37 pKa = 4.08 KK38 pKa = 10.82 GGYY41 pKa = 8.12 RR42 pKa = 11.84 NVVLRR47 pKa = 11.84 ANGEE51 pKa = 4.05 KK52 pKa = 10.03 INRR55 pKa = 11.84 KK56 pKa = 5.78 VHH58 pKa = 5.99 RR59 pKa = 11.84 LVAEE63 pKa = 4.24 AFLPNPRR70 pKa = 11.84 GLPQVNHH77 pKa = 6.71 KK78 pKa = 11.25 DD79 pKa = 3.38 EE80 pKa = 4.58 VRR82 pKa = 11.84 DD83 pKa = 3.82 NNHH86 pKa = 5.13 VSNLEE91 pKa = 4.01 WCDD94 pKa = 3.09 CAYY97 pKa = 10.99 NVDD100 pKa = 3.7 YY101 pKa = 10.99 SQAATYY107 pKa = 10.63 IVMCPKK113 pKa = 10.79 GNILVVRR120 pKa = 11.84 NVAKK124 pKa = 10.43 FCRR127 pKa = 11.84 EE128 pKa = 3.75 NGLSSGSFTDD138 pKa = 3.32 MLKK141 pKa = 10.59 GRR143 pKa = 11.84 RR144 pKa = 11.84 KK145 pKa = 6.36 QHH147 pKa = 5.17 KK148 pKa = 9.51 GYY150 pKa = 10.76 RR151 pKa = 11.84 LVV153 pKa = 3.34
Molecular weight: 17.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 9.37
IPC_protein 9.487
Toseland 10.087
ProMoST 9.78
Dawson 10.277
Bjellqvist 9.97
Wikipedia 10.452
Rodwell 10.657
Grimsley 10.35
Solomon 10.321
Lehninger 10.292
Nozaki 10.145
DTASelect 9.955
Thurlkill 10.145
EMBOSS 10.496
Sillero 10.204
Patrickios 10.292
IPC_peptide 10.321
IPC2_peptide 8.873
IPC2.peptide.svr19 8.295
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
13991
32
1267
254.4
28.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.206 ± 0.599
1.058 ± 0.168
5.954 ± 0.222
7.055 ± 0.351
3.574 ± 0.161
7.669 ± 0.387
2.13 ± 0.266
5.375 ± 0.212
6.504 ± 0.292
7.962 ± 0.216
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.909 ± 0.19
4.51 ± 0.229
3.402 ± 0.186
4.146 ± 0.269
5.425 ± 0.26
6.004 ± 0.367
5.346 ± 0.312
7.069 ± 0.293
1.329 ± 0.129
3.374 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here