Burkholderia multivorans (strain ATCC 17616 / 249)
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6040 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H3KJQ0|A0A0H3KJQ0_BURM1 Permease of the major facilitator superfamily OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=BMULJ_01715 PE=4 SV=1
MM1 pKa = 7.36 TFSLNPSEE9 pKa = 5.42 LFDD12 pKa = 4.94 GFDD15 pKa = 3.78 SFDD18 pKa = 3.6 GFDD21 pKa = 4.19 GFDD24 pKa = 3.5 EE25 pKa = 4.72 PMHH28 pKa = 6.53 DD29 pKa = 3.31 HH30 pKa = 7.19 AGTPEE35 pKa = 3.96 EE36 pKa = 4.06 AHH38 pKa = 5.23 VTSYY42 pKa = 10.98 QAVLQEE48 pKa = 4.25 PMVSLVGSVDD58 pKa = 3.67 RR59 pKa = 11.84 PDD61 pKa = 3.5 VASVAILGYY70 pKa = 10.35 NN71 pKa = 3.21
Molecular weight: 7.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.516
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.427
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.846
Patrickios 1.875
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.76
Protein with the highest isoelectric point:
>tr|A0A0H3KBX0|A0A0H3KBX0_BURM1 DNA polymerase III subunit delta OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=holA PE=4 SV=1
MM1 pKa = 7.98 PFRR4 pKa = 11.84 LTSRR8 pKa = 11.84 LSRR11 pKa = 11.84 AAKK14 pKa = 9.58 RR15 pKa = 11.84 VVPSAPARR23 pKa = 11.84 PLRR26 pKa = 11.84 HH27 pKa = 5.45 HH28 pKa = 6.27 RR29 pKa = 11.84 TRR31 pKa = 11.84 VRR33 pKa = 11.84 ALAEE37 pKa = 3.88 RR38 pKa = 11.84 THH40 pKa = 6.33 ALSRR44 pKa = 11.84 LDD46 pKa = 5.1 GIRR49 pKa = 11.84 AWFHH53 pKa = 6.5 AFLSLMSAQALARR66 pKa = 11.84 RR67 pKa = 11.84 AGLRR71 pKa = 11.84 SLLQKK76 pKa = 10.34 PSSLRR81 pKa = 11.84 ALALRR86 pKa = 11.84 RR87 pKa = 11.84 AQAPQRR93 pKa = 11.84 RR94 pKa = 11.84 VNRR97 pKa = 11.84 PRR99 pKa = 11.84 RR100 pKa = 11.84 LAASNGWFGFGGRR113 pKa = 4.19
Molecular weight: 12.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.486
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.384
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.157
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6040
0
6040
1951710
25
4531
323.1
34.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.854 ± 0.05
0.932 ± 0.012
5.801 ± 0.032
4.723 ± 0.03
3.599 ± 0.021
8.194 ± 0.053
2.323 ± 0.017
4.52 ± 0.024
2.828 ± 0.028
10.137 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.237 ± 0.014
2.528 ± 0.022
5.295 ± 0.025
3.33 ± 0.019
7.486 ± 0.041
5.169 ± 0.04
5.422 ± 0.042
7.788 ± 0.031
1.399 ± 0.015
2.435 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here