Mucor ambiguus
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11311 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C9MKJ6|A0A0C9MKJ6_9FUNG Within the bgcn gene intron protein OS=Mucor ambiguus OX=91626 GN=MAM1_0045c03112 PE=3 SV=1
MM1 pKa = 7.8 IYY3 pKa = 10.75 YY4 pKa = 10.43 LFLIHH9 pKa = 7.38 DD10 pKa = 4.01 VNGDD14 pKa = 3.56 GFLDD18 pKa = 3.83 GHH20 pKa = 6.09 EE21 pKa = 4.3 LRR23 pKa = 11.84 AAFTDD28 pKa = 4.18 FGDD31 pKa = 3.68 EE32 pKa = 4.79 HH33 pKa = 7.27 EE34 pKa = 5.84 DD35 pKa = 3.15 ATQYY39 pKa = 11.57 LSLEE43 pKa = 4.47 EE44 pKa = 3.92 VTEE47 pKa = 4.26 MVDD50 pKa = 4.02 HH51 pKa = 6.57 VLEE54 pKa = 4.46 EE55 pKa = 5.28 DD56 pKa = 4.67 DD57 pKa = 5.1 LDD59 pKa = 5.75 GDD61 pKa = 4.74 GRR63 pKa = 11.84 ISWSEE68 pKa = 3.72 YY69 pKa = 10.69 LEE71 pKa = 4.35 SQSYY75 pKa = 9.17 HH76 pKa = 5.14 QDD78 pKa = 2.99 KK79 pKa = 11.27
Molecular weight: 9.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.656
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.783
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.973
Patrickios 0.947
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A0C9MKN7|A0A0C9MKN7_9FUNG ATP dependent helicase OS=Mucor ambiguus OX=91626 GN=MAM1_0046d03142 PE=3 SV=1
MM1 pKa = 7.63 GNTHH5 pKa = 5.46 STTTRR10 pKa = 11.84 RR11 pKa = 11.84 HH12 pKa = 4.74 AAPRR16 pKa = 11.84 AVKK19 pKa = 8.93 TRR21 pKa = 11.84 KK22 pKa = 8.79 SPRR25 pKa = 11.84 RR26 pKa = 11.84 FGLPSQKK33 pKa = 9.46 RR34 pKa = 11.84 AHH36 pKa = 6.55 AAAPVAVKK44 pKa = 9.84 PRR46 pKa = 11.84 RR47 pKa = 11.84 KK48 pKa = 9.73 FGFGSSRR55 pKa = 11.84 KK56 pKa = 8.39 HH57 pKa = 4.37 RR58 pKa = 11.84 HH59 pKa = 4.38 GAAPVATTTVPSNSMTLRR77 pKa = 11.84 KK78 pKa = 9.52 LKK80 pKa = 10.81 AKK82 pKa = 10.19 ISPGPKK88 pKa = 8.99 VANTNKK94 pKa = 10.11 RR95 pKa = 11.84 SRR97 pKa = 11.84 KK98 pKa = 8.03 NAKK101 pKa = 9.01 KK102 pKa = 10.49 DD103 pKa = 3.49 PVSGIVNKK111 pKa = 10.19 LAPTVGATGASAAPRR126 pKa = 11.84 HH127 pKa = 5.95 RR128 pKa = 11.84 SRR130 pKa = 11.84 GFGFGRR136 pKa = 11.84 RR137 pKa = 11.84 RR138 pKa = 11.84 PVEE141 pKa = 3.67 PPITTKK147 pKa = 10.57 RR148 pKa = 11.84 GMFSRR153 pKa = 11.84 FQRR156 pKa = 11.84 ARR158 pKa = 11.84 AYY160 pKa = 10.3
Molecular weight: 17.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.067
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.457
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.164
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.073
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11311
0
11311
5257899
27
6210
464.8
52.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.274 ± 0.021
1.346 ± 0.008
5.83 ± 0.016
6.081 ± 0.025
3.869 ± 0.014
5.03 ± 0.022
2.665 ± 0.011
5.777 ± 0.017
6.108 ± 0.021
8.895 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.501 ± 0.009
4.865 ± 0.015
4.946 ± 0.017
4.979 ± 0.027
4.78 ± 0.016
8.449 ± 0.031
6.274 ± 0.02
5.918 ± 0.017
1.112 ± 0.006
3.297 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here