Trichinella pseudospiralis (Parasitic roundworm)
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14914 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0V0W537|A0A0V0W537_TRIPS Uncharacterized protein OS=Trichinella pseudospiralis OX=6337 GN=T4E_10601 PE=4 SV=1
MM1 pKa = 7.5 AGVPPFDD8 pKa = 3.28 VPSFNFYY15 pKa = 10.84 VEE17 pKa = 4.45 SIKK20 pKa = 10.4 ILPKK24 pKa = 10.34 NFVHH28 pKa = 6.13 RR29 pKa = 11.84 TYY31 pKa = 10.93 NIQTEE36 pKa = 4.52 AEE38 pKa = 4.01 EE39 pKa = 4.52 STRR42 pKa = 11.84 SSQLYY47 pKa = 7.92 EE48 pKa = 3.96 AEE50 pKa = 4.46 YY51 pKa = 10.2 PMGMYY56 pKa = 10.07 NPKK59 pKa = 10.1 QPFEE63 pKa = 4.11 VEE65 pKa = 3.57 RR66 pKa = 11.84 FEE68 pKa = 4.44 IRR70 pKa = 11.84 DD71 pKa = 3.5 GAGEE75 pKa = 4.05 DD76 pKa = 3.88 VEE78 pKa = 4.97 EE79 pKa = 4.21 LAAVQQHH86 pKa = 5.86 EE87 pKa = 4.63 EE88 pKa = 4.3 DD89 pKa = 3.76 FGDD92 pKa = 3.7 RR93 pKa = 11.84 SPAEE97 pKa = 4.38 EE98 pKa = 4.26 YY99 pKa = 9.47 TSSVASYY106 pKa = 11.47 SNASFNDD113 pKa = 3.6 DD114 pKa = 4.12 DD115 pKa = 6.94 DD116 pKa = 5.31 EE117 pKa = 7.19 DD118 pKa = 4.8 EE119 pKa = 5.63 DD120 pKa = 4.3 DD121 pKa = 4.48 ASSIEE126 pKa = 3.6 ADD128 pKa = 3.85 YY129 pKa = 11.41 YY130 pKa = 11.39 FEE132 pKa = 4.31
Molecular weight: 15.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.859
IPC_protein 3.808
Toseland 3.63
ProMoST 3.923
Dawson 3.783
Bjellqvist 3.973
Wikipedia 3.668
Rodwell 3.643
Grimsley 3.541
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.05
Thurlkill 3.656
EMBOSS 3.681
Sillero 3.923
Patrickios 1.85
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A0V0WJS3|A0A0V0WJS3_TRIPS Uncharacterized protein OS=Trichinella pseudospiralis OX=6337 GN=T4E_3400 PE=4 SV=1
LL1 pKa = 7.21 ARR3 pKa = 11.84 GPPVRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 SKK12 pKa = 9.79 LTTPAPVPRR21 pKa = 11.84 WTRR24 pKa = 11.84 ATSTSTWSARR34 pKa = 11.84 TVTTRR39 pKa = 11.84 RR40 pKa = 11.84 AGRR43 pKa = 11.84 FRR45 pKa = 11.84 TCRR48 pKa = 11.84 PSRR51 pKa = 11.84 SRR53 pKa = 11.84 NTSAHH58 pKa = 6.25 RR59 pKa = 11.84 RR60 pKa = 11.84 SQPSWCPRR68 pKa = 11.84 SNIRR72 pKa = 11.84 TLGQLPMTTTRR83 pKa = 11.84 WRR85 pKa = 11.84 WKK87 pKa = 10.25 RR88 pKa = 11.84 MPLRR92 pKa = 11.84 SRR94 pKa = 11.84 CRR96 pKa = 11.84 ILTTSATRR104 pKa = 11.84 TSISTSTAAASAARR118 pKa = 11.84 RR119 pKa = 11.84 PTLRR123 pKa = 11.84 TAPP126 pKa = 3.71
Molecular weight: 14.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.31
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.032
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.151
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10629
4285
14914
6968983
30
12697
467.3
52.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.39 ± 0.016
2.7 ± 0.018
5.267 ± 0.014
6.407 ± 0.027
4.586 ± 0.016
4.951 ± 0.023
2.424 ± 0.008
5.599 ± 0.016
6.063 ± 0.02
9.86 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.438 ± 0.009
5.044 ± 0.012
4.241 ± 0.021
4.305 ± 0.016
5.431 ± 0.016
8.274 ± 0.02
5.219 ± 0.015
6.397 ± 0.013
1.207 ± 0.006
3.145 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here