Xanthomonas phage phiXv2

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4QHX2|A0A2Z4QHX2_9VIRU Replication initiation protein OS=Xanthomonas phage phiXv2 OX=2201472 PE=4 SV=1
MM1 pKa = 6.52ITVRR5 pKa = 11.84LVACILAVALWSCAFSANAAMCRR28 pKa = 11.84DD29 pKa = 3.58AADD32 pKa = 4.16ACDD35 pKa = 3.34QGQAFLAARR44 pKa = 11.84MLADD48 pKa = 3.26QRR50 pKa = 11.84GVDD53 pKa = 3.69LCKK56 pKa = 10.56LVGGSNSLYY65 pKa = 10.45KK66 pKa = 11.0GPDD69 pKa = 3.14VVAEE73 pKa = 3.98SAGIYY78 pKa = 9.08NAQATCSIGGPAGAGSTFYY97 pKa = 11.28SKK99 pKa = 9.39TCAQRR104 pKa = 11.84PPLIGASSSDD114 pKa = 3.3GSGFSCDD121 pKa = 4.58DD122 pKa = 3.36GCFYY126 pKa = 11.22NFTVGASGGSGMYY139 pKa = 10.05PSGATCSAGDD149 pKa = 4.66APPSTPGDD157 pKa = 3.73DD158 pKa = 4.8GGDD161 pKa = 3.51GDD163 pKa = 4.7GGSDD167 pKa = 3.22GGGDD171 pKa = 3.53GGSDD175 pKa = 3.17GGGDD179 pKa = 3.53GGSDD183 pKa = 3.17GGGDD187 pKa = 3.57GGSDD191 pKa = 3.23GGSDD195 pKa = 3.81GGGDD199 pKa = 3.74GDD201 pKa = 5.07GDD203 pKa = 4.06GDD205 pKa = 4.55GDD207 pKa = 4.55GDD209 pKa = 4.29GDD211 pKa = 4.77GDD213 pKa = 4.51GDD215 pKa = 4.1TPGDD219 pKa = 3.86GDD221 pKa = 4.04GTTPGQGEE229 pKa = 4.5GGEE232 pKa = 4.19GAPMSEE238 pKa = 5.38LYY240 pKa = 10.52NKK242 pKa = 10.02SGKK245 pKa = 7.31TVEE248 pKa = 4.36SVLSKK253 pKa = 10.98FNTQVRR259 pKa = 11.84GTPMVAGITDD269 pKa = 4.56FMTVPSGGSCPVFSLGASKK288 pKa = 8.92WWNAMTINFHH298 pKa = 6.93CGGDD302 pKa = 3.5FLAFLRR308 pKa = 11.84AAGWVILAIAAYY320 pKa = 8.93AALRR324 pKa = 11.84IAVTT328 pKa = 3.72

Molecular weight: 31.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4QI91|A0A2Z4QI91_9VIRU Helix-turn-helix XRE-family DNA binding protein OS=Xanthomonas phage phiXv2 OX=2201472 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84WCPPVNRR10 pKa = 11.84LASAAATCSSVVANTACMRR29 pKa = 11.84WCKK32 pKa = 10.19KK33 pKa = 10.21RR34 pKa = 11.84SPCPARR40 pKa = 11.84RR41 pKa = 11.84TPP43 pKa = 3.41

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

1894

33

440

157.8

17.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.827 ± 1.126

2.059 ± 0.633

5.861 ± 1.034

3.749 ± 0.648

4.224 ± 0.561

10.718 ± 2.371

1.637 ± 0.48

3.643 ± 0.452

4.435 ± 0.706

7.233 ± 1.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.491

2.429 ± 0.429

4.857 ± 0.519

3.115 ± 0.524

6.969 ± 1.281

6.23 ± 0.863

5.702 ± 0.678

7.392 ± 0.692

2.64 ± 0.514

2.587 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski