Thioflavicoccus mobilis 8321
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3528 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0GW24|L0GW24_9GAMM FeS assembly protein SufB OS=Thioflavicoccus mobilis 8321 OX=765912 GN=Thimo_2216 PE=3 SV=1
MM1 pKa = 7.43 LPIILALCLFAGATQADD18 pKa = 3.88 DD19 pKa = 5.06 ALIQEE24 pKa = 4.74 YY25 pKa = 10.49 LSAPALPPASLTDD38 pKa = 3.46 DD39 pKa = 3.63 TVEE42 pKa = 4.12 PEE44 pKa = 3.75 VTIIEE49 pKa = 4.36 RR50 pKa = 11.84 GQDD53 pKa = 3.04 VIYY56 pKa = 9.59 EE57 pKa = 4.0 YY58 pKa = 10.53 RR59 pKa = 11.84 VRR61 pKa = 11.84 GQLYY65 pKa = 7.95 MVKK68 pKa = 9.18 VQPIAGPTYY77 pKa = 10.59 YY78 pKa = 11.27 LLDD81 pKa = 3.74 TDD83 pKa = 5.35 GDD85 pKa = 4.18 GTLDD89 pKa = 3.58 TRR91 pKa = 11.84 DD92 pKa = 3.83 DD93 pKa = 4.05 RR94 pKa = 11.84 PLNMATQQWLLFDD107 pKa = 3.89 WW108 pKa = 4.61
Molecular weight: 12.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.795
IPC_protein 3.757
Toseland 3.541
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.151
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.884
Patrickios 1.888
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|L0GT22|L0GT22_9GAMM Mg-chelatase subunit ChlD OS=Thioflavicoccus mobilis 8321 OX=765912 GN=Thimo_1115 PE=4 SV=1
MM1 pKa = 7.58 SLPSSEE7 pKa = 4.11 VRR9 pKa = 11.84 SPSATAEE16 pKa = 4.06 PPTADD21 pKa = 3.16 RR22 pKa = 11.84 SGRR25 pKa = 11.84 AHH27 pKa = 5.93 LRR29 pKa = 11.84 EE30 pKa = 3.87 RR31 pKa = 11.84 AIRR34 pKa = 11.84 WLIRR38 pKa = 11.84 LIARR42 pKa = 11.84 LPLAWIHH49 pKa = 6.45 RR50 pKa = 11.84 LGATLSQLIVWLPNRR65 pKa = 11.84 QRR67 pKa = 11.84 RR68 pKa = 11.84 NALINIATCLPDD80 pKa = 4.25 LSPTEE85 pKa = 4.17 QVALRR90 pKa = 11.84 NANLRR95 pKa = 11.84 EE96 pKa = 3.86 FGKK99 pKa = 9.04 TYY101 pKa = 11.06 LEE103 pKa = 5.0 IGHH106 pKa = 7.1 LWLRR110 pKa = 11.84 PVDD113 pKa = 3.83 EE114 pKa = 4.35 VLGLIRR120 pKa = 11.84 EE121 pKa = 4.27 VRR123 pKa = 11.84 GAEE126 pKa = 3.8 LLRR129 pKa = 11.84 RR130 pKa = 11.84 HH131 pKa = 6.03 GGKK134 pKa = 10.36 GLIVLSPHH142 pKa = 5.56 IGAWEE147 pKa = 3.66 LAGLYY152 pKa = 10.48 LSIQGPTAIFYY163 pKa = 10.47 KK164 pKa = 8.66 PQKK167 pKa = 10.32 YY168 pKa = 10.43 LDD170 pKa = 4.15 DD171 pKa = 5.69 LIVAARR177 pKa = 11.84 RR178 pKa = 11.84 RR179 pKa = 11.84 GGARR183 pKa = 11.84 LAPITAKK190 pKa = 10.6 GIRR193 pKa = 11.84 VLVQALEE200 pKa = 3.93 RR201 pKa = 11.84 GEE203 pKa = 4.07 YY204 pKa = 10.42 VGILPDD210 pKa = 4.57 QEE212 pKa = 4.13 PKK214 pKa = 9.25 MDD216 pKa = 3.8 KK217 pKa = 10.82 GAVFAPFFGIPALTMLLVNRR237 pKa = 11.84 LARR240 pKa = 11.84 RR241 pKa = 11.84 TGAPVIFMFAEE252 pKa = 4.27 RR253 pKa = 11.84 LPRR256 pKa = 11.84 ARR258 pKa = 11.84 GFRR261 pKa = 11.84 IHH263 pKa = 7.07 CLPAPDD269 pKa = 5.76 GIDD272 pKa = 3.93 SADD275 pKa = 3.6 DD276 pKa = 3.67 HH277 pKa = 6.15 QAAAALNRR285 pKa = 11.84 GIEE288 pKa = 4.02 QCIGVCPAQFVWPYY302 pKa = 9.83 KK303 pKa = 9.44 RR304 pKa = 11.84 FRR306 pKa = 11.84 RR307 pKa = 11.84 RR308 pKa = 11.84 PEE310 pKa = 4.19 GQPKK314 pKa = 10.53 LYY316 pKa = 10.23 TGPLDD321 pKa = 4.71 DD322 pKa = 4.81 AQTLARR328 pKa = 11.84 LASDD332 pKa = 3.2 QVGRR336 pKa = 11.84 GTPTT340 pKa = 2.81
Molecular weight: 37.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.56
IPC_protein 10.555
Toseland 10.584
ProMoST 10.409
Dawson 10.701
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.745
Grimsley 10.76
Solomon 10.862
Lehninger 10.804
Nozaki 10.599
DTASelect 10.467
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.643
Patrickios 10.423
IPC_peptide 10.862
IPC2_peptide 9.648
IPC2.peptide.svr19 8.626
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3528
0
3528
1133450
28
2948
321.3
35.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.049 ± 0.045
1.022 ± 0.014
5.891 ± 0.029
6.316 ± 0.042
3.335 ± 0.025
8.328 ± 0.036
2.239 ± 0.019
4.714 ± 0.029
2.467 ± 0.029
11.358 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.015 ± 0.018
2.237 ± 0.021
5.467 ± 0.033
3.43 ± 0.024
8.338 ± 0.046
4.804 ± 0.032
4.948 ± 0.031
7.222 ± 0.036
1.435 ± 0.019
2.386 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here