Pistacia emaravirus
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A410JAQ1|A0A410JAQ1_9VIRU p6 protein OS=Pistacia emaravirus OX=2507689 PE=4 SV=1
MM1 pKa = 7.49 EE2 pKa = 5.01 FKK4 pKa = 10.89 SYY6 pKa = 10.77 VRR8 pKa = 11.84 TFNACRR14 pKa = 11.84 KK15 pKa = 9.76 GNVSDD20 pKa = 4.26 YY21 pKa = 10.98 YY22 pKa = 10.56 IEE24 pKa = 5.06 FDD26 pKa = 3.29 HH27 pKa = 6.93 QLFFSIVEE35 pKa = 4.07 RR36 pKa = 11.84 MDD38 pKa = 3.62 TEE40 pKa = 4.11 THH42 pKa = 5.97 DD43 pKa = 3.06 QFLRR47 pKa = 11.84 FGEE50 pKa = 4.75 LYY52 pKa = 10.65 DD53 pKa = 5.43 DD54 pKa = 4.5 GNIDD58 pKa = 3.13 IPEE61 pKa = 4.13 IGRR64 pKa = 11.84 TVNEE68 pKa = 4.26 IVLDD72 pKa = 4.77 DD73 pKa = 3.41 ITIKK77 pKa = 10.35 KK78 pKa = 9.99 KK79 pKa = 10.18 AMRR82 pKa = 11.84 YY83 pKa = 7.34 HH84 pKa = 6.22 FRR86 pKa = 11.84 YY87 pKa = 9.46 IQILHH92 pKa = 6.77 SILGIVDD99 pKa = 4.08 AFNDD103 pKa = 3.44 EE104 pKa = 4.3 MSPDD108 pKa = 3.14 FHH110 pKa = 9.21 IFGTLVLKK118 pKa = 11.01 NKK120 pKa = 9.77 LLPFIPDD127 pKa = 3.25 NSDD130 pKa = 3.49 CLLLSFDD137 pKa = 4.93 IIKK140 pKa = 10.59 NLIWEE145 pKa = 4.23 IVIGYY150 pKa = 9.72 DD151 pKa = 4.03 PIDD154 pKa = 3.95 IIEE157 pKa = 4.56 KK158 pKa = 9.63 YY159 pKa = 10.19 KK160 pKa = 10.75 SRR162 pKa = 11.84 QLKK165 pKa = 9.47 SVIYY169 pKa = 10.88 NEE171 pKa = 4.27 DD172 pKa = 3.06 TVAYY176 pKa = 8.84 QFQVLAISYY185 pKa = 8.42 LAIRR189 pKa = 11.84 ASSMVDD195 pKa = 3.02 KK196 pKa = 10.63 EE197 pKa = 3.92 KK198 pKa = 11.31 SKK200 pKa = 11.24 NKK202 pKa = 10.29 SYY204 pKa = 11.38 NDD206 pKa = 3.56 SNQVTIEE213 pKa = 4.46 DD214 pKa = 4.03 GNKK217 pKa = 10.05 LSEE220 pKa = 4.67 LEE222 pKa = 4.19 SDD224 pKa = 3.95 SKK226 pKa = 11.5 SIASSSHH233 pKa = 5.91 QDD235 pKa = 3.35 DD236 pKa = 4.76 LMSKK240 pKa = 10.39
Molecular weight: 27.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.831
IPC2_protein 4.863
IPC_protein 4.813
Toseland 4.685
ProMoST 4.952
Dawson 4.8
Bjellqvist 4.94
Wikipedia 4.698
Rodwell 4.685
Grimsley 4.596
Solomon 4.8
Lehninger 4.749
Nozaki 4.914
DTASelect 5.118
Thurlkill 4.698
EMBOSS 4.724
Sillero 4.965
Patrickios 3.935
IPC_peptide 4.8
IPC2_peptide 4.952
IPC2.peptide.svr19 4.935
Protein with the highest isoelectric point:
>tr|A0A410JAN1|A0A410JAN1_9VIRU p5 variant B OS=Pistacia emaravirus OX=2507689 PE=4 SV=1
MM1 pKa = 7.49 IHH3 pKa = 5.9 NQIIKK8 pKa = 9.85 KK9 pKa = 7.87 QRR11 pKa = 11.84 KK12 pKa = 7.22 KK13 pKa = 10.8 QLVSYY18 pKa = 9.43 LAKK21 pKa = 9.74 NANMEE26 pKa = 4.45 HH27 pKa = 6.6 IKK29 pKa = 10.43 PYY31 pKa = 8.71 TNSIGGCTIKK41 pKa = 10.72 LSNKK45 pKa = 9.49 EE46 pKa = 4.24 KK47 pKa = 10.31 ISDD50 pKa = 3.8 YY51 pKa = 10.95 KK52 pKa = 11.02 GLSSFEE58 pKa = 4.05 EE59 pKa = 4.17 NIYY62 pKa = 10.72 NQIKK66 pKa = 10.8 NKK68 pKa = 9.95 FNNCEE73 pKa = 4.1 CFEE76 pKa = 4.74 KK77 pKa = 10.89 DD78 pKa = 3.47 VFFDD82 pKa = 4.42 EE83 pKa = 5.04 DD84 pKa = 3.63 SKK86 pKa = 11.03 MPKK89 pKa = 9.36 NASISYY95 pKa = 10.25 IEE97 pKa = 5.18 INDD100 pKa = 3.28 QFPRR104 pKa = 11.84 YY105 pKa = 8.62 MYY107 pKa = 9.15 TIKK110 pKa = 10.75 KK111 pKa = 8.38 MRR113 pKa = 11.84 SKK115 pKa = 10.96 YY116 pKa = 10.2 KK117 pKa = 10.68 YY118 pKa = 10.01 SLTNIDD124 pKa = 5.17 DD125 pKa = 4.36 LLFSIIKK132 pKa = 9.51 NHH134 pKa = 5.3 LHH136 pKa = 5.61 NFIIRR141 pKa = 11.84 EE142 pKa = 4.01 FEE144 pKa = 3.89 SDD146 pKa = 3.04 INEE149 pKa = 3.57 INIIIKK155 pKa = 10.42 GFLHH159 pKa = 6.54 IPKK162 pKa = 9.4 QFVMVKK168 pKa = 10.04 INNKK172 pKa = 9.37 NLLINDD178 pKa = 4.1 TKK180 pKa = 11.43 YY181 pKa = 10.07 NWEE184 pKa = 4.39 NIISSTTLCDD194 pKa = 3.34 LRR196 pKa = 11.84 SILSHH201 pKa = 6.77 FDD203 pKa = 3.36 DD204 pKa = 4.7 MRR206 pKa = 11.84 DD207 pKa = 3.62 FYY209 pKa = 11.11 YY210 pKa = 10.88 KK211 pKa = 10.23 KK212 pKa = 10.46 VQGTNIVKK220 pKa = 10.32 SIYY223 pKa = 10.91 SFINDD228 pKa = 3.14 NKK230 pKa = 10.92 SLINHH235 pKa = 6.16 VLPSIKK241 pKa = 9.69 TSFKK245 pKa = 10.64 TYY247 pKa = 10.46 DD248 pKa = 3.61 DD249 pKa = 4.44 CVLKK253 pKa = 10.76 YY254 pKa = 10.58 INAHH258 pKa = 5.56 LKK260 pKa = 9.61 KK261 pKa = 10.49 AYY263 pKa = 10.17 NEE265 pKa = 4.38 SNYY268 pKa = 11.04 SLVFDD273 pKa = 4.3 SSTIQEE279 pKa = 4.19 FLIEE283 pKa = 4.37 TEE285 pKa = 4.2 SCSDD289 pKa = 3.58 FSLEE293 pKa = 3.7 VRR295 pKa = 11.84 KK296 pKa = 9.98 AIHH299 pKa = 6.69 EE300 pKa = 4.35 DD301 pKa = 3.14 VTKK304 pKa = 10.54 QLEE307 pKa = 4.21 GLKK310 pKa = 8.49 TQFSEE315 pKa = 4.11 YY316 pKa = 10.42 DD317 pKa = 3.4 ILKK320 pKa = 8.92 MPCLEE325 pKa = 4.6 LVNQRR330 pKa = 11.84 LVPIKK335 pKa = 10.52 HH336 pKa = 6.31 NKK338 pKa = 8.91 NKK340 pKa = 10.48 LNVSGSCEE348 pKa = 3.71 QSVAFPLNIDD358 pKa = 4.52 EE359 pKa = 4.35 IVKK362 pKa = 9.98 YY363 pKa = 10.11 PPGEE367 pKa = 4.02 NPIQDD372 pKa = 3.38 NFYY375 pKa = 10.75 YY376 pKa = 10.75 GLPNTLEE383 pKa = 4.09 ISTQYY388 pKa = 10.98 LNSAMRR394 pKa = 11.84 KK395 pKa = 9.36 IIQEE399 pKa = 4.11 EE400 pKa = 4.12 KK401 pKa = 10.73 DD402 pKa = 3.36 IDD404 pKa = 4.92 DD405 pKa = 3.56 IDD407 pKa = 4.64 YY408 pKa = 10.83 IAGYY412 pKa = 8.27 PFKK415 pKa = 10.43 RR416 pKa = 11.84 SYY418 pKa = 10.7 KK419 pKa = 9.75 ILIFSILKK427 pKa = 8.3 KK428 pKa = 9.86 QNYY431 pKa = 9.07 NIRR434 pKa = 11.84 ISHH437 pKa = 6.93 LFLHH441 pKa = 5.91 YY442 pKa = 10.19 FCYY445 pKa = 10.56 YY446 pKa = 9.47 MYY448 pKa = 10.05 AASVASIRR456 pKa = 11.84 VGVDD460 pKa = 2.86 MIYY463 pKa = 10.23 EE464 pKa = 3.98 QKK466 pKa = 10.82 QIDD469 pKa = 3.75 RR470 pKa = 11.84 ARR472 pKa = 11.84 EE473 pKa = 3.69 FSKK476 pKa = 11.16 KK477 pKa = 10.04 NISVIRR483 pKa = 11.84 NLVQSHH489 pKa = 5.55 KK490 pKa = 10.53 HH491 pKa = 5.96 LFLQNQCVSITLAA504 pKa = 3.73
Molecular weight: 59.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.855
IPC2_protein 8.039
IPC_protein 7.878
Toseland 7.98
ProMoST 8.434
Dawson 8.712
Bjellqvist 8.814
Wikipedia 8.741
Rodwell 8.799
Grimsley 8.039
Solomon 8.785
Lehninger 8.756
Nozaki 8.916
DTASelect 8.639
Thurlkill 8.726
EMBOSS 8.873
Sillero 8.96
Patrickios 4.139
IPC_peptide 8.77
IPC2_peptide 7.541
IPC2.peptide.svr19 7.77
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5314
240
2299
664.3
77.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.632 ± 0.558
1.844 ± 0.37
6.361 ± 0.388
6.022 ± 0.481
4.686 ± 0.307
3.049 ± 0.44
2.465 ± 0.208
10.181 ± 0.502
8.863 ± 0.286
8.863 ± 0.554
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.011 ± 0.374
7.49 ± 0.396
3.105 ± 0.164
2.616 ± 0.256
3.406 ± 0.188
7.697 ± 0.42
5.702 ± 0.496
4.535 ± 0.457
0.452 ± 0.149
6.022 ± 0.515
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here