Burkholderia virus DC1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lessievirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6NW24|I6NW24_9CAUD Uncharacterized protein OS=Burkholderia virus DC1 OX=1136535 GN=DC1_00035 PE=4 SV=1
MM1 pKa = 7.0KK2 pKa = 10.3QLYY5 pKa = 8.33TKK7 pKa = 10.63AGLRR11 pKa = 11.84IVGTKK16 pKa = 9.37EE17 pKa = 3.98VINATALVSGFDD29 pKa = 3.61DD30 pKa = 3.9HH31 pKa = 6.76GQPIYY36 pKa = 10.86AGSTDD41 pKa = 5.11LDD43 pKa = 3.48WDD45 pKa = 4.06SQLSEE50 pKa = 4.04TDD52 pKa = 2.96EE53 pKa = 4.28KK54 pKa = 11.58GNYY57 pKa = 9.19ILIDD61 pKa = 3.61EE62 pKa = 5.37DD63 pKa = 4.65GEE65 pKa = 4.28EE66 pKa = 4.27HH67 pKa = 7.46SPDD70 pKa = 3.31EE71 pKa = 4.95CEE73 pKa = 5.57LRR75 pKa = 11.84DD76 pKa = 3.55AA77 pKa = 5.46

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6NRM0|I6NRM0_9CAUD Uncharacterized protein OS=Burkholderia virus DC1 OX=1136535 GN=DC1_00024 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 4.72AQSQNRR8 pKa = 11.84IILDD12 pKa = 3.34HH13 pKa = 6.71LKK15 pKa = 10.3EE16 pKa = 4.25VGPITPLEE24 pKa = 3.95ALRR27 pKa = 11.84LHH29 pKa = 7.05GIMRR33 pKa = 11.84LGARR37 pKa = 11.84VHH39 pKa = 5.68EE40 pKa = 4.34LRR42 pKa = 11.84EE43 pKa = 4.26GGHH46 pKa = 6.44NIVTEE51 pKa = 3.93IVKK54 pKa = 10.46VKK56 pKa = 9.96GRR58 pKa = 11.84KK59 pKa = 8.03GSKK62 pKa = 7.4PARR65 pKa = 11.84VARR68 pKa = 11.84YY69 pKa = 9.78SLVKK73 pKa = 10.35AAAA76 pKa = 3.73

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

19256

29

4650

263.8

28.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.211 ± 0.493

0.717 ± 0.2

6.486 ± 0.231

6.652 ± 0.317

3.147 ± 0.158

8.003 ± 0.347

1.844 ± 0.195

4.528 ± 0.253

4.622 ± 0.283

7.665 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.109

3.35 ± 0.192

5.572 ± 0.223

4.232 ± 0.296

7.296 ± 0.29

4.689 ± 0.15

5.5 ± 0.203

6.185 ± 0.221

1.34 ± 0.112

2.446 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski