Anaerofilum sp. An201

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Anaerofilum; unclassified Anaerofilum

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2953 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4HAG2|A0A1Y4HAG2_9FIRM NADH-quinone oxidoreductase subunit F OS=Anaerofilum sp. An201 OX=1965588 GN=B5F36_04575 PE=3 SV=1
MM1 pKa = 7.55EE2 pKa = 5.61RR3 pKa = 11.84FCATCGTRR11 pKa = 11.84LEE13 pKa = 4.94PPAEE17 pKa = 4.16SCPCCGRR24 pKa = 11.84QIDD27 pKa = 4.18WDD29 pKa = 3.94AASQPLSFVKK39 pKa = 10.58QEE41 pKa = 4.28SVPAAQADD49 pKa = 3.62ADD51 pKa = 3.97AGAEE55 pKa = 4.14GSLEE59 pKa = 3.88EE60 pKa = 5.3LEE62 pKa = 5.21EE63 pKa = 5.19KK64 pKa = 10.22DD65 pKa = 4.13TVCGQPGEE73 pKa = 4.63AEE75 pKa = 4.28PEE77 pKa = 4.05PDD79 pKa = 3.99ASEE82 pKa = 4.97ADD84 pKa = 3.59AQEE87 pKa = 4.38EE88 pKa = 4.13LSADD92 pKa = 3.04VDD94 pKa = 4.27APCDD98 pKa = 3.61LQLEE102 pKa = 4.51YY103 pKa = 10.73LSQEE107 pKa = 4.09PAPAPLEE114 pKa = 4.1FSDD117 pKa = 4.06AVVMCIYY124 pKa = 11.21AMVPLAGLILLTLVMLFSDD143 pKa = 3.57EE144 pKa = 3.8EE145 pKa = 4.13DD146 pKa = 3.38RR147 pKa = 11.84NRR149 pKa = 11.84RR150 pKa = 11.84TLAKK154 pKa = 10.01ALLVAHH160 pKa = 7.12LGLMLLLVVGWFALRR175 pKa = 11.84PLVASYY181 pKa = 10.49MMSGYY186 pKa = 11.29GMLGG190 pKa = 3.0

Molecular weight: 20.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4GZF0|A0A1Y4GZF0_9FIRM Capsular biosynthesis protein OS=Anaerofilum sp. An201 OX=1965588 GN=B5F36_12885 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.59KK9 pKa = 7.38RR10 pKa = 11.84HH11 pKa = 5.81RR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.07GFLNRR21 pKa = 11.84MSTKK25 pKa = 10.25NGRR28 pKa = 11.84KK29 pKa = 9.11VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.24GRR39 pKa = 11.84AKK41 pKa = 10.54LAII44 pKa = 4.11

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2953

0

2953

938666

26

2747

317.9

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.451 ± 0.068

1.783 ± 0.024

5.488 ± 0.039

6.612 ± 0.041

3.769 ± 0.03

7.761 ± 0.041

1.843 ± 0.02

5.119 ± 0.037

4.336 ± 0.036

10.095 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.734 ± 0.02

3.201 ± 0.038

4.416 ± 0.03

3.895 ± 0.031

5.97 ± 0.053

5.415 ± 0.035

5.333 ± 0.038

7.129 ± 0.041

1.208 ± 0.02

3.443 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski