Amino acid dipepetide frequency for Anaerofilum sp. An201

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.336AlaAla: 15.336 ± 0.235
1.721AlaCys: 1.721 ± 0.043
6.206AlaAsp: 6.206 ± 0.087
6.897AlaGlu: 6.897 ± 0.103
3.632AlaPhe: 3.632 ± 0.068
9.027AlaGly: 9.027 ± 0.127
1.744AlaHis: 1.744 ± 0.042
4.684AlaIle: 4.684 ± 0.076
3.975AlaLys: 3.975 ± 0.069
10.78AlaLeu: 10.78 ± 0.129
2.83AlaMet: 2.83 ± 0.057
2.545AlaAsn: 2.545 ± 0.053
4.056AlaPro: 4.056 ± 0.086
4.152AlaGln: 4.152 ± 0.078
5.797AlaArg: 5.797 ± 0.088
4.857AlaSer: 4.857 ± 0.074
3.802AlaThr: 3.802 ± 0.078
8.748AlaVal: 8.748 ± 0.122
1.05AlaTrp: 1.05 ± 0.035
2.673AlaTyr: 2.673 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
1.887CysAla: 1.887 ± 0.052
0.396CysCys: 0.396 ± 0.023
0.919CysAsp: 0.919 ± 0.028
1.03CysGlu: 1.03 ± 0.033
0.647CysPhe: 0.647 ± 0.027
1.905CysGly: 1.905 ± 0.046
0.34CysHis: 0.34 ± 0.019
0.948CysIle: 0.948 ± 0.028
0.65CysLys: 0.65 ± 0.029
1.473CysLeu: 1.473 ± 0.042
0.478CysMet: 0.478 ± 0.024
0.482CysAsn: 0.482 ± 0.024
0.918CysPro: 0.918 ± 0.034
0.473CysGln: 0.473 ± 0.023
1.331CysArg: 1.331 ± 0.038
0.989CysSer: 0.989 ± 0.034
1.032CysThr: 1.032 ± 0.033
1.174CysVal: 1.174 ± 0.042
0.209CysTrp: 0.209 ± 0.013
0.551CysTyr: 0.551 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
5.422AspAla: 5.422 ± 0.077
0.917AspCys: 0.917 ± 0.036
2.817AspAsp: 2.817 ± 0.074
4.39AspGlu: 4.39 ± 0.076
2.364AspPhe: 2.364 ± 0.049
5.037AspGly: 5.037 ± 0.082
0.947AspHis: 0.947 ± 0.039
3.174AspIle: 3.174 ± 0.061
2.23AspLys: 2.23 ± 0.052
4.87AspLeu: 4.87 ± 0.061
1.414AspMet: 1.414 ± 0.041
1.569AspAsn: 1.569 ± 0.045
2.583AspPro: 2.583 ± 0.057
1.479AspGln: 1.479 ± 0.041
3.0AspArg: 3.0 ± 0.062
2.924AspSer: 2.924 ± 0.054
3.246AspThr: 3.246 ± 0.059
3.634AspVal: 3.634 ± 0.063
0.75AspTrp: 0.75 ± 0.027
2.118AspTyr: 2.118 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
5.889GluAla: 5.889 ± 0.09
0.821GluCys: 0.821 ± 0.032
3.463GluAsp: 3.463 ± 0.063
5.065GluGlu: 5.065 ± 0.084
2.283GluPhe: 2.283 ± 0.05
4.545GluGly: 4.545 ± 0.072
1.252GluHis: 1.252 ± 0.038
3.59GluIle: 3.59 ± 0.073
4.23GluLys: 4.23 ± 0.075
6.27GluLeu: 6.27 ± 0.099
2.23GluMet: 2.23 ± 0.045
3.128GluAsn: 3.128 ± 0.065
2.289GluPro: 2.289 ± 0.06
3.52GluGln: 3.52 ± 0.072
3.527GluArg: 3.527 ± 0.072
3.0GluSer: 3.0 ± 0.064
3.586GluThr: 3.586 ± 0.07
3.995GluVal: 3.995 ± 0.065
0.795GluTrp: 0.795 ± 0.032
2.646GluTyr: 2.646 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.948PheAla: 3.948 ± 0.072
0.89PheCys: 0.89 ± 0.033
2.408PheAsp: 2.408 ± 0.053
2.298PheGlu: 2.298 ± 0.055
1.657PhePhe: 1.657 ± 0.048
3.265PheGly: 3.265 ± 0.065
0.69PheHis: 0.69 ± 0.029
1.765PheIle: 1.765 ± 0.051
1.081PheLys: 1.081 ± 0.037
3.702PheLeu: 3.702 ± 0.074
0.771PheMet: 0.771 ± 0.033
1.057PheAsn: 1.057 ± 0.038
1.36PhePro: 1.36 ± 0.038
1.048PheGln: 1.048 ± 0.036
1.943PheArg: 1.943 ± 0.042
2.771PheSer: 2.771 ± 0.056
2.26PheThr: 2.26 ± 0.047
2.867PheVal: 2.867 ± 0.06
0.513PheTrp: 0.513 ± 0.025
1.395PheTyr: 1.395 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
6.945GlyAla: 6.945 ± 0.108
1.652GlyCys: 1.652 ± 0.051
3.804GlyAsp: 3.804 ± 0.068
5.146GlyGlu: 5.146 ± 0.08
3.021GlyPhe: 3.021 ± 0.06
6.135GlyGly: 6.135 ± 0.102
1.452GlyHis: 1.452 ± 0.038
4.561GlyIle: 4.561 ± 0.077
4.07GlyLys: 4.07 ± 0.075
7.392GlyLeu: 7.392 ± 0.104
2.653GlyMet: 2.653 ± 0.054
2.397GlyAsn: 2.397 ± 0.07
2.069GlyPro: 2.069 ± 0.044
3.019GlyGln: 3.019 ± 0.065
4.821GlyArg: 4.821 ± 0.08
4.38GlySer: 4.38 ± 0.077
4.116GlyThr: 4.116 ± 0.076
5.826GlyVal: 5.826 ± 0.076
1.178GlyTrp: 1.178 ± 0.037
2.968GlyTyr: 2.968 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.501HisAla: 1.501 ± 0.039
0.384HisCys: 0.384 ± 0.02
0.867HisAsp: 0.867 ± 0.033
0.965HisGlu: 0.965 ± 0.028
0.79HisPhe: 0.79 ± 0.034
1.449HisGly: 1.449 ± 0.043
0.467HisHis: 0.467 ± 0.024
1.164HisIle: 1.164 ± 0.037
0.74HisLys: 0.74 ± 0.031
1.804HisLeu: 1.804 ± 0.048
0.426HisMet: 0.426 ± 0.022
0.674HisAsn: 0.674 ± 0.028
1.2HisPro: 1.2 ± 0.035
0.585HisGln: 0.585 ± 0.024
1.177HisArg: 1.177 ± 0.038
1.05HisSer: 1.05 ± 0.038
1.206HisThr: 1.206 ± 0.036
1.012HisVal: 1.012 ± 0.028
0.281HisTrp: 0.281 ± 0.016
0.685HisTyr: 0.685 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.05IleAla: 5.05 ± 0.082
1.043IleCys: 1.043 ± 0.035
3.104IleAsp: 3.104 ± 0.056
3.222IleGlu: 3.222 ± 0.063
1.787IlePhe: 1.787 ± 0.048
3.714IleGly: 3.714 ± 0.08
1.042IleHis: 1.042 ± 0.038
2.413IleIle: 2.413 ± 0.056
1.813IleLys: 1.813 ± 0.054
5.408IleLeu: 5.408 ± 0.089
1.024IleMet: 1.024 ± 0.035
1.614IleAsn: 1.614 ± 0.044
2.656IlePro: 2.656 ± 0.059
1.882IleGln: 1.882 ± 0.047
3.361IleArg: 3.361 ± 0.07
3.094IleSer: 3.094 ± 0.062
3.189IleThr: 3.189 ± 0.062
3.597IleVal: 3.597 ± 0.073
0.496IleTrp: 0.496 ± 0.025
1.678IleTyr: 1.678 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
4.372LysAla: 4.372 ± 0.079
0.484LysCys: 0.484 ± 0.02
2.252LysAsp: 2.252 ± 0.05
3.069LysGlu: 3.069 ± 0.072
1.151LysPhe: 1.151 ± 0.037
3.053LysGly: 3.053 ± 0.06
0.707LysHis: 0.707 ± 0.028
2.289LysIle: 2.289 ± 0.057
2.996LysLys: 2.996 ± 0.073
3.94LysLeu: 3.94 ± 0.065
1.255LysMet: 1.255 ± 0.042
1.945LysAsn: 1.945 ± 0.046
1.77LysPro: 1.77 ± 0.049
1.864LysGln: 1.864 ± 0.052
2.545LysArg: 2.545 ± 0.064
2.198LysSer: 2.198 ± 0.056
2.705LysThr: 2.705 ± 0.06
2.769LysVal: 2.769 ± 0.069
0.44LysTrp: 0.44 ± 0.022
1.553LysTyr: 1.553 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
11.333LeuAla: 11.333 ± 0.162
2.434LeuCys: 2.434 ± 0.059
5.871LeuAsp: 5.871 ± 0.08
6.584LeuGlu: 6.584 ± 0.095
3.961LeuPhe: 3.961 ± 0.076
6.885LeuGly: 6.885 ± 0.103
2.051LeuHis: 2.051 ± 0.05
4.097LeuIle: 4.097 ± 0.085
3.813LeuLys: 3.813 ± 0.075
11.003LeuLeu: 11.003 ± 0.166
2.262LeuMet: 2.262 ± 0.058
2.832LeuAsn: 2.832 ± 0.057
5.33LeuPro: 5.33 ± 0.08
3.413LeuGln: 3.413 ± 0.066
6.288LeuArg: 6.288 ± 0.12
6.29LeuSer: 6.29 ± 0.089
5.064LeuThr: 5.064 ± 0.065
6.815LeuVal: 6.815 ± 0.102
1.173LeuTrp: 1.173 ± 0.044
3.446LeuTyr: 3.446 ± 0.075
0.0LeuXaa: 0.0 ± 0.0
Met
2.84MetAla: 2.84 ± 0.058
0.338MetCys: 0.338 ± 0.018
1.564MetAsp: 1.564 ± 0.049
1.975MetGlu: 1.975 ± 0.044
0.802MetPhe: 0.802 ± 0.034
2.006MetGly: 2.006 ± 0.046
0.437MetHis: 0.437 ± 0.02
1.246MetIle: 1.246 ± 0.041
1.66MetLys: 1.66 ± 0.045
2.871MetLeu: 2.871 ± 0.055
0.843MetMet: 0.843 ± 0.035
1.063MetAsn: 1.063 ± 0.037
1.35MetPro: 1.35 ± 0.039
1.313MetGln: 1.313 ± 0.038
1.464MetArg: 1.464 ± 0.043
1.425MetSer: 1.425 ± 0.04
1.421MetThr: 1.421 ± 0.037
1.766MetVal: 1.766 ± 0.05
0.226MetTrp: 0.226 ± 0.015
0.728MetTyr: 0.728 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.99AsnAla: 2.99 ± 0.058
0.551AsnCys: 0.551 ± 0.024
1.496AsnAsp: 1.496 ± 0.042
1.893AsnGlu: 1.893 ± 0.05
1.187AsnPhe: 1.187 ± 0.04
3.049AsnGly: 3.049 ± 0.067
0.613AsnHis: 0.613 ± 0.029
2.026AsnIle: 2.026 ± 0.05
1.242AsnLys: 1.242 ± 0.038
3.104AsnLeu: 3.104 ± 0.062
0.85AsnMet: 0.85 ± 0.032
1.153AsnAsn: 1.153 ± 0.045
1.799AsnPro: 1.799 ± 0.05
1.025AsnGln: 1.025 ± 0.034
1.855AsnArg: 1.855 ± 0.048
1.668AsnSer: 1.668 ± 0.051
1.896AsnThr: 1.896 ± 0.051
2.026AsnVal: 2.026 ± 0.05
0.392AsnTrp: 0.392 ± 0.021
1.191AsnTyr: 1.191 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
5.608ProAla: 5.608 ± 0.108
0.67ProCys: 0.67 ± 0.029
3.198ProAsp: 3.198 ± 0.054
3.69ProGlu: 3.69 ± 0.074
1.737ProPhe: 1.737 ± 0.043
3.499ProGly: 3.499 ± 0.074
0.769ProHis: 0.769 ± 0.03
1.748ProIle: 1.748 ± 0.045
1.477ProLys: 1.477 ± 0.047
4.033ProLeu: 4.033 ± 0.07
1.015ProMet: 1.015 ± 0.032
1.241ProAsn: 1.241 ± 0.038
1.691ProPro: 1.691 ± 0.049
1.666ProGln: 1.666 ± 0.047
2.028ProArg: 2.028 ± 0.048
2.305ProSer: 2.305 ± 0.05
2.082ProThr: 2.082 ± 0.077
3.821ProVal: 3.821 ± 0.068
0.422ProTrp: 0.422 ± 0.022
1.457ProTyr: 1.457 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
3.875GlnAla: 3.875 ± 0.069
0.436GlnCys: 0.436 ± 0.023
1.841GlnAsp: 1.841 ± 0.052
2.319GlnGlu: 2.319 ± 0.059
1.229GlnPhe: 1.229 ± 0.036
2.382GlnGly: 2.382 ± 0.055
0.634GlnHis: 0.634 ± 0.029
2.011GlnIle: 2.011 ± 0.044
2.22GlnLys: 2.22 ± 0.06
4.464GlnLeu: 4.464 ± 0.065
1.236GlnMet: 1.236 ± 0.037
1.515GlnAsn: 1.515 ± 0.043
1.976GlnPro: 1.976 ± 0.058
2.1GlnGln: 2.1 ± 0.058
2.271GlnArg: 2.271 ± 0.053
1.885GlnSer: 1.885 ± 0.044
2.168GlnThr: 2.168 ± 0.043
2.511GlnVal: 2.511 ± 0.055
0.476GlnTrp: 0.476 ± 0.023
1.405GlnTyr: 1.405 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
5.56ArgAla: 5.56 ± 0.089
1.086ArgCys: 1.086 ± 0.037
2.726ArgAsp: 2.726 ± 0.058
3.999ArgGlu: 3.999 ± 0.07
2.39ArgPhe: 2.39 ± 0.047
3.649ArgGly: 3.649 ± 0.068
1.109ArgHis: 1.109 ± 0.038
3.39ArgIle: 3.39 ± 0.067
2.676ArgLys: 2.676 ± 0.065
6.391ArgLeu: 6.391 ± 0.098
2.022ArgMet: 2.022 ± 0.051
1.653ArgAsn: 1.653 ± 0.038
2.533ArgPro: 2.533 ± 0.059
2.741ArgGln: 2.741 ± 0.066
5.048ArgArg: 5.048 ± 0.101
2.806ArgSer: 2.806 ± 0.059
3.18ArgThr: 3.18 ± 0.062
3.765ArgVal: 3.765 ± 0.066
0.845ArgTrp: 0.845 ± 0.034
2.135ArgTyr: 2.135 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
5.593SerAla: 5.593 ± 0.074
0.852SerCys: 0.852 ± 0.033
2.824SerAsp: 2.824 ± 0.06
3.18SerGlu: 3.18 ± 0.066
2.331SerPhe: 2.331 ± 0.047
4.982SerGly: 4.982 ± 0.083
0.991SerHis: 0.991 ± 0.037
2.988SerIle: 2.988 ± 0.059
1.829SerLys: 1.829 ± 0.052
5.125SerLeu: 5.125 ± 0.078
1.525SerMet: 1.525 ± 0.041
1.564SerAsn: 1.564 ± 0.047
2.432SerPro: 2.432 ± 0.057
1.893SerGln: 1.893 ± 0.042
3.498SerArg: 3.498 ± 0.068
3.201SerSer: 3.201 ± 0.088
2.772SerThr: 2.772 ± 0.054
3.952SerVal: 3.952 ± 0.061
0.711SerTrp: 0.711 ± 0.03
1.904SerTyr: 1.904 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
6.218ThrAla: 6.218 ± 0.109
0.627ThrCys: 0.627 ± 0.026
2.906ThrAsp: 2.906 ± 0.061
3.134ThrGlu: 3.134 ± 0.067
1.926ThrPhe: 1.926 ± 0.045
4.946ThrGly: 4.946 ± 0.071
0.951ThrHis: 0.951 ± 0.033
2.971ThrIle: 2.971 ± 0.058
1.768ThrLys: 1.768 ± 0.047
5.415ThrLeu: 5.415 ± 0.086
1.402ThrMet: 1.402 ± 0.038
1.471ThrAsn: 1.471 ± 0.044
2.993ThrPro: 2.993 ± 0.066
1.676ThrGln: 1.676 ± 0.043
2.612ThrArg: 2.612 ± 0.054
2.618ThrSer: 2.618 ± 0.061
2.628ThrThr: 2.628 ± 0.063
4.961ThrVal: 4.961 ± 0.089
0.561ThrTrp: 0.561 ± 0.028
1.545ThrTyr: 1.545 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
5.941ValAla: 5.941 ± 0.095
1.745ValCys: 1.745 ± 0.052
3.83ValAsp: 3.83 ± 0.061
4.432ValGlu: 4.432 ± 0.067
2.873ValPhe: 2.873 ± 0.055
4.649ValGly: 4.649 ± 0.081
1.226ValHis: 1.226 ± 0.038
3.739ValIle: 3.739 ± 0.069
3.03ValLys: 3.03 ± 0.058
8.05ValLeu: 8.05 ± 0.116
1.915ValMet: 1.915 ± 0.043
2.23ValAsn: 2.23 ± 0.057
3.19ValPro: 3.19 ± 0.064
3.097ValGln: 3.097 ± 0.058
4.211ValArg: 4.211 ± 0.073
4.177ValSer: 4.177 ± 0.063
4.136ValThr: 4.136 ± 0.076
5.26ValVal: 5.26 ± 0.082
1.009ValTrp: 1.009 ± 0.034
2.548ValTyr: 2.548 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
1.006TrpAla: 1.006 ± 0.033
0.228TrpCys: 0.228 ± 0.017
0.664TrpAsp: 0.664 ± 0.031
0.734TrpGlu: 0.734 ± 0.03
0.507TrpPhe: 0.507 ± 0.023
0.817TrpGly: 0.817 ± 0.03
0.268TrpHis: 0.268 ± 0.019
0.528TrpIle: 0.528 ± 0.024
0.576TrpLys: 0.576 ± 0.024
1.383TrpLeu: 1.383 ± 0.042
0.427TrpMet: 0.427 ± 0.024
0.554TrpAsn: 0.554 ± 0.028
0.467TrpPro: 0.467 ± 0.024
0.722TrpGln: 0.722 ± 0.032
0.705TrpArg: 0.705 ± 0.025
0.537TrpSer: 0.537 ± 0.026
0.568TrpThr: 0.568 ± 0.024
0.723TrpVal: 0.723 ± 0.033
0.178TrpTrp: 0.178 ± 0.015
0.485TrpTyr: 0.485 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.195TyrAla: 3.195 ± 0.06
0.557TyrCys: 0.557 ± 0.024
2.123TyrAsp: 2.123 ± 0.054
2.102TyrGlu: 2.102 ± 0.049
1.416TyrPhe: 1.416 ± 0.042
2.612TyrGly: 2.612 ± 0.056
0.738TyrHis: 0.738 ± 0.028
1.849TyrIle: 1.849 ± 0.045
1.281TyrLys: 1.281 ± 0.042
3.468TyrLeu: 3.468 ± 0.07
0.76TyrMet: 0.76 ± 0.027
1.32TyrAsn: 1.32 ± 0.041
1.497TyrPro: 1.497 ± 0.041
1.286TyrGln: 1.286 ± 0.044
2.222TyrArg: 2.222 ± 0.057
1.972TyrSer: 1.972 ± 0.055
2.276TyrThr: 2.276 ± 0.052
2.066TyrVal: 2.066 ± 0.05
0.372TyrTrp: 0.372 ± 0.024
1.321TyrTyr: 1.321 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2953 proteins (938666 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski