Wenzhou yanvirus-like virus 2
Average proteome isoelectric point is 7.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLD2|A0A1L3KLD2_9VIRU Uncharacterized protein OS=Wenzhou yanvirus-like virus 2 OX=1923684 PE=4 SV=1
MM1 pKa = 7.92 SITTHH6 pKa = 6.06 KK7 pKa = 9.61 KK8 pKa = 9.53 KK9 pKa = 10.56 CGSHH13 pKa = 6.81 PDD15 pKa = 3.41 VKK17 pKa = 10.64 RR18 pKa = 11.84 GAEE21 pKa = 3.5 QVLFQKK27 pKa = 10.71 YY28 pKa = 7.75 HH29 pKa = 4.84 GKK31 pKa = 9.9 YY32 pKa = 8.36 GRR34 pKa = 11.84 SRR36 pKa = 11.84 RR37 pKa = 11.84 SYY39 pKa = 10.02 LKK41 pKa = 10.54 RR42 pKa = 11.84 GFGPDD47 pKa = 3.42 GGPDD51 pKa = 2.76 RR52 pKa = 11.84 AVALVHH58 pKa = 6.71 RR59 pKa = 11.84 LYY61 pKa = 11.09 AGLRR65 pKa = 11.84 GAGRR69 pKa = 11.84 YY70 pKa = 7.52 PVPQNPSDD78 pKa = 3.71 ILNHH82 pKa = 6.4 LLATNVIDD90 pKa = 3.84 GRR92 pKa = 11.84 YY93 pKa = 9.02 RR94 pKa = 11.84 DD95 pKa = 3.92 EE96 pKa = 4.36 EE97 pKa = 4.22 KK98 pKa = 11.05 GGLGLPCSLTAFATAFPNQRR118 pKa = 11.84 HH119 pKa = 5.35 ICLFYY124 pKa = 11.14 EE125 pKa = 4.46 KK126 pKa = 10.51 LCEE129 pKa = 4.22 QGLHH133 pKa = 6.04 EE134 pKa = 4.89 EE135 pKa = 4.3 NSRR138 pKa = 11.84 GPSGGMTLTGMVSALQEE155 pKa = 3.89 WEE157 pKa = 3.94 AGIIADD163 pKa = 4.52 PKK165 pKa = 9.39 PKK167 pKa = 9.98 VVRR170 pKa = 11.84 PRR172 pKa = 11.84 GMDD175 pKa = 3.32 LHH177 pKa = 6.48 QVNCRR182 pKa = 11.84 GGTFLSYY189 pKa = 11.03 DD190 pKa = 3.62 VVHH193 pKa = 6.19 RR194 pKa = 11.84 TLLRR198 pKa = 11.84 DD199 pKa = 3.49 GARR202 pKa = 11.84 EE203 pKa = 3.79 QIIVYY208 pKa = 10.39 INDD211 pKa = 3.16 EE212 pKa = 4.3 SCNFGHH218 pKa = 6.62 FCAVSSLNEE227 pKa = 3.76 DD228 pKa = 2.95 AMKK231 pKa = 10.81 RR232 pKa = 11.84 EE233 pKa = 3.66 IAKK236 pKa = 9.79 GKK238 pKa = 10.06 RR239 pKa = 11.84 RR240 pKa = 11.84 AEE242 pKa = 4.11 YY243 pKa = 10.89 ADD245 pKa = 3.64 MVAADD250 pKa = 3.95 SDD252 pKa = 4.09 QVPKK256 pKa = 10.6 EE257 pKa = 4.11 VAPKK261 pKa = 10.23 AISGDD266 pKa = 3.92 HH267 pKa = 6.14 IVPATTSEE275 pKa = 4.27 PGPSGINQEE284 pKa = 4.48 AEE286 pKa = 4.08 THH288 pKa = 6.11 HH289 pKa = 6.23 SQEE292 pKa = 3.72 LDD294 pKa = 3.26 ALLEE298 pKa = 3.92 EE299 pKa = 5.93 AIAEE303 pKa = 4.25 MQGMDD308 pKa = 4.51 PPQPPPPEE316 pKa = 4.82 PNDD319 pKa = 3.4 NGPGGNIGGEE329 pKa = 4.03 GDD331 pKa = 4.52 GPDD334 pKa = 3.69 EE335 pKa = 4.14 PPIIKK340 pKa = 10.34 EE341 pKa = 4.14 EE342 pKa = 4.24 VPPPPPPPPPEE353 pKa = 4.25 PKK355 pKa = 9.75 PIPVSRR361 pKa = 11.84 RR362 pKa = 11.84 TLRR365 pKa = 11.84 YY366 pKa = 9.38 LRR368 pKa = 11.84 EE369 pKa = 3.67 KK370 pKa = 11.23 LEE372 pKa = 4.46 FIKK375 pKa = 9.33 THH377 pKa = 5.83 SATPILDD384 pKa = 3.56 HH385 pKa = 6.82 RR386 pKa = 11.84 PVRR389 pKa = 11.84 AYY391 pKa = 10.32 ACAGLVSEE399 pKa = 5.09 GCVEE403 pKa = 4.49 HH404 pKa = 7.39 EE405 pKa = 4.51 DD406 pKa = 3.78 ASHH409 pKa = 6.4 EE410 pKa = 4.34 GGVGLTEE417 pKa = 3.97 LHH419 pKa = 6.75 PEE421 pKa = 3.65 VLARR425 pKa = 11.84 FNFTRR430 pKa = 11.84 RR431 pKa = 11.84 SYY433 pKa = 10.35 YY434 pKa = 9.78 YY435 pKa = 8.23 VTAGVEE441 pKa = 3.92 PDD443 pKa = 3.47 ALDD446 pKa = 4.56 GRR448 pKa = 11.84 FLTNGQYY455 pKa = 10.56 NPAWVASIQFEE466 pKa = 4.31 DD467 pKa = 3.75 TAFTLEE473 pKa = 4.01 FVGNKK478 pKa = 9.61 GPCDD482 pKa = 3.5 YY483 pKa = 11.19 YY484 pKa = 11.15 RR485 pKa = 11.84 LYY487 pKa = 10.83 KK488 pKa = 10.57 AATLGAKK495 pKa = 9.79 KK496 pKa = 10.64 GFFSCPDD503 pKa = 4.68 FINEE507 pKa = 4.18 ALDD510 pKa = 5.4 DD511 pKa = 4.09 VSAWAAEE518 pKa = 4.12 IGLITQPQATIKK530 pKa = 10.59 HH531 pKa = 4.66 VTNLPGRR538 pKa = 11.84 GLRR541 pKa = 11.84 PRR543 pKa = 11.84 SLKK546 pKa = 10.46 GVAGASRR553 pKa = 11.84 AQWAMHH559 pKa = 6.24 ARR561 pKa = 11.84 HH562 pKa = 6.25 APEE565 pKa = 4.66 DD566 pKa = 3.51 LRR568 pKa = 11.84 GPYY571 pKa = 9.82 HH572 pKa = 7.15 SLFIEE577 pKa = 4.37 NADD580 pKa = 4.96 DD581 pKa = 4.69 SNSDD585 pKa = 3.55 PNEE588 pKa = 3.57 VLKK591 pKa = 10.88 ALKK594 pKa = 10.1 YY595 pKa = 9.47 EE596 pKa = 3.87 KK597 pKa = 11.29 AMMEE601 pKa = 4.15 KK602 pKa = 9.68 EE603 pKa = 3.87 HH604 pKa = 6.64 GKK606 pKa = 10.2 RR607 pKa = 11.84 GYY609 pKa = 9.57 CAPKK613 pKa = 9.08 MSTPKK618 pKa = 9.27 TCASCGVSPPKK629 pKa = 10.57 GKK631 pKa = 10.71 YY632 pKa = 8.04 KK633 pKa = 9.94 WKK635 pKa = 10.1 HH636 pKa = 4.92 RR637 pKa = 11.84 QCKK640 pKa = 10.1 DD641 pKa = 3.2 CQQKK645 pKa = 10.8 LKK647 pKa = 9.71 TCGAVSTMGQQIQQNLTVAEE667 pKa = 4.07 GHH669 pKa = 6.83 PGCVHH674 pKa = 6.74 LNSSTLPPKK683 pKa = 9.87 KK684 pKa = 10.15 EE685 pKa = 3.47 KK686 pKa = 8.47 WAKK689 pKa = 8.66 VHH691 pKa = 6.11 IPKK694 pKa = 10.47 GAIKK698 pKa = 9.75 VHH700 pKa = 6.44 KK701 pKa = 10.43 SDD703 pKa = 4.53 VPWLRR708 pKa = 11.84 HH709 pKa = 5.65 ADD711 pKa = 3.12 WGGSKK716 pKa = 8.22 MHH718 pKa = 6.24 SVEE721 pKa = 5.46 KK722 pKa = 10.55 EE723 pKa = 3.92 DD724 pKa = 4.56 LSKK727 pKa = 10.54 IDD729 pKa = 3.61 TTLEE733 pKa = 3.81 RR734 pKa = 11.84 PKK736 pKa = 10.46 RR737 pKa = 11.84 EE738 pKa = 3.98 CVLAGIGVSGCYY750 pKa = 10.11 PMVTRR755 pKa = 11.84 KK756 pKa = 10.05 GFYY759 pKa = 10.85 ARR761 pKa = 11.84 MQALIGRR768 pKa = 11.84 AYY770 pKa = 9.78 LQKK773 pKa = 10.52 PEE775 pKa = 4.29 SSPAAWEE782 pKa = 4.48 AMNQMKK788 pKa = 10.47 DD789 pKa = 3.36 CLLPNDD795 pKa = 4.64 ALDD798 pKa = 4.09 GEE800 pKa = 4.68 KK801 pKa = 10.7 FSIDD805 pKa = 3.12 FWLTTMPARR814 pKa = 11.84 RR815 pKa = 11.84 RR816 pKa = 11.84 RR817 pKa = 11.84 ALEE820 pKa = 4.04 RR821 pKa = 11.84 AHH823 pKa = 7.26 KK824 pKa = 9.64 EE825 pKa = 3.97 YY826 pKa = 10.76 KK827 pKa = 10.27 EE828 pKa = 4.16 SGGLRR833 pKa = 11.84 DD834 pKa = 3.79 KK835 pKa = 11.34 DD836 pKa = 3.58 LTFSAFVKK844 pKa = 10.61 QEE846 pKa = 3.72 LLAGYY851 pKa = 10.04 KK852 pKa = 10.19 KK853 pKa = 10.76 FGWADD858 pKa = 3.61 AKK860 pKa = 10.75 PLAEE864 pKa = 4.85 SIARR868 pKa = 11.84 MIMAPKK874 pKa = 10.23 DD875 pKa = 3.36 KK876 pKa = 10.78 AHH878 pKa = 6.46 IVAGPVIKK886 pKa = 10.4 PKK888 pKa = 10.76 LEE890 pKa = 3.56 RR891 pKa = 11.84 LKK893 pKa = 10.92 RR894 pKa = 11.84 HH895 pKa = 6.15 WGPDD899 pKa = 2.42 NWLFYY904 pKa = 11.12 GATTPEE910 pKa = 4.63 KK911 pKa = 10.04 LQSWLDD917 pKa = 3.55 SCITGCEE924 pKa = 4.04 DD925 pKa = 3.94 GEE927 pKa = 5.23 VFAFWCDD934 pKa = 3.29 YY935 pKa = 11.96 SMFDD939 pKa = 3.92 CTHH942 pKa = 6.13 SAEE945 pKa = 4.18 SMEE948 pKa = 4.29 LVEE951 pKa = 4.94 SFYY954 pKa = 11.32 SEE956 pKa = 3.91 MRR958 pKa = 11.84 TDD960 pKa = 3.48 PEE962 pKa = 3.77 FARR965 pKa = 11.84 LINAWRR971 pKa = 11.84 APRR974 pKa = 11.84 GKK976 pKa = 9.44 MGEE979 pKa = 3.92 LSYY982 pKa = 10.82 RR983 pKa = 11.84 AAIMLASGRR992 pKa = 11.84 DD993 pKa = 3.57 DD994 pKa = 3.51 TSLMNAILNGLVMGLSVAAAVAGVEE1019 pKa = 4.16 LEE1021 pKa = 4.26 SLQKK1025 pKa = 9.97 EE1026 pKa = 4.3 HH1027 pKa = 7.02 LRR1029 pKa = 11.84 FAEE1032 pKa = 4.3 AYY1034 pKa = 9.26 IRR1036 pKa = 11.84 ISICGDD1042 pKa = 3.11 DD1043 pKa = 3.78 TLGFLPKK1050 pKa = 10.28 HH1051 pKa = 6.06 LWPDD1055 pKa = 3.38 RR1056 pKa = 11.84 ARR1058 pKa = 11.84 IMRR1061 pKa = 11.84 DD1062 pKa = 2.45 IQTNIARR1069 pKa = 11.84 FGLVPKK1075 pKa = 10.39 LDD1077 pKa = 3.77 CTNFLGSAVYY1087 pKa = 10.53 LGMRR1091 pKa = 11.84 PYY1093 pKa = 10.87 DD1094 pKa = 3.45 VPTPTGRR1101 pKa = 11.84 KK1102 pKa = 7.14 WLWGRR1107 pKa = 11.84 TVGRR1111 pKa = 11.84 AAYY1114 pKa = 9.46 KK1115 pKa = 9.79 FGWMLDD1121 pKa = 3.61 PSKK1124 pKa = 11.33 GDD1126 pKa = 3.31 AAAWATGVADD1136 pKa = 3.64 SVVRR1140 pKa = 11.84 TQPYY1144 pKa = 9.86 VPILSDD1150 pKa = 4.56 LARR1153 pKa = 11.84 QTLKK1157 pKa = 10.64 LRR1159 pKa = 11.84 EE1160 pKa = 4.18 GARR1163 pKa = 11.84 RR1164 pKa = 11.84 TPVAFEE1170 pKa = 4.19 AGKK1173 pKa = 9.1 PWTHH1177 pKa = 3.94 WTVRR1181 pKa = 11.84 EE1182 pKa = 4.04 EE1183 pKa = 4.35 IKK1185 pKa = 11.06 NLTYY1189 pKa = 10.49 DD1190 pKa = 3.34 RR1191 pKa = 11.84 KK1192 pKa = 8.95 TIEE1195 pKa = 4.59 ALVKK1199 pKa = 10.49 SYY1201 pKa = 8.65 STPTLYY1207 pKa = 11.04 GGDD1210 pKa = 3.8 EE1211 pKa = 4.78 PICVPSVEE1219 pKa = 4.36 DD1220 pKa = 4.13 FYY1222 pKa = 11.44 RR1223 pKa = 11.84 TIATIQAIPCLPYY1236 pKa = 10.94 VIDD1239 pKa = 4.7 DD1240 pKa = 3.76 LALRR1244 pKa = 11.84 FMCVTDD1250 pKa = 3.72 DD1251 pKa = 3.77 CC1252 pKa = 6.43
Molecular weight: 139.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.909
IPC2_protein 7.263
IPC_protein 7.073
Toseland 6.868
ProMoST 7.717
Dawson 7.775
Bjellqvist 8.214
Wikipedia 7.673
Rodwell 7.79
Grimsley 6.956
Solomon 7.79
Lehninger 7.805
Nozaki 8.463
DTASelect 7.951
Thurlkill 7.995
EMBOSS 8.009
Sillero 8.317
Patrickios 4.329
IPC_peptide 7.79
IPC2_peptide 7.337
IPC2.peptide.svr19 7.2
Protein with the highest isoelectric point:
>tr|A0A1L3KLD2|A0A1L3KLD2_9VIRU Uncharacterized protein OS=Wenzhou yanvirus-like virus 2 OX=1923684 PE=4 SV=1
MM1 pKa = 7.35 SHH3 pKa = 6.77 TGLKK7 pKa = 10.42 SLDD10 pKa = 4.15 EE11 pKa = 4.22 IAQTICLPNEE21 pKa = 4.01 RR22 pKa = 11.84 APVRR26 pKa = 11.84 LPTYY30 pKa = 10.14 PSIDD34 pKa = 3.02 KK35 pKa = 9.25 TATFRR40 pKa = 11.84 YY41 pKa = 9.7 RR42 pKa = 11.84 YY43 pKa = 10.05 QNTQSLRR50 pKa = 11.84 DD51 pKa = 3.49 EE52 pKa = 5.29 ADD54 pKa = 3.53 PSDD57 pKa = 3.84 QNVLGRR63 pKa = 11.84 KK64 pKa = 9.0 RR65 pKa = 11.84 FLVTRR70 pKa = 11.84 DD71 pKa = 3.21 PAAPLLLDD79 pKa = 4.09 TVHH82 pKa = 6.96 TLQKK86 pKa = 10.3 AISGNPSIYY95 pKa = 9.67 TGSVLARR102 pKa = 11.84 TEE104 pKa = 3.86 PVNINTADD112 pKa = 3.84 EE113 pKa = 4.39 LTTATVTLSKK123 pKa = 9.89 MYY125 pKa = 9.57 PWEE128 pKa = 4.37 FYY130 pKa = 10.84 NRR132 pKa = 11.84 LPSGEE137 pKa = 4.32 YY138 pKa = 9.95 DD139 pKa = 3.3 SKK141 pKa = 11.37 QWFVVPKK148 pKa = 9.69 TLDD151 pKa = 3.26 SYY153 pKa = 11.72 GRR155 pKa = 11.84 ASGFLGHH162 pKa = 7.28 LGVALFVTDD171 pKa = 5.07 IAPQTQPIVTYY182 pKa = 10.52 KK183 pKa = 10.68 PGDD186 pKa = 3.76 PVSEE190 pKa = 4.2 IPVGALVLDD199 pKa = 3.77 YY200 pKa = 10.29 TLTIEE205 pKa = 4.58 IVDD208 pKa = 3.89 STGVAGSMICDD219 pKa = 4.05 LDD221 pKa = 3.74 YY222 pKa = 11.27 TKK224 pKa = 10.44 TRR226 pKa = 11.84 EE227 pKa = 4.19 APGTYY232 pKa = 9.13 TGFFIIDD239 pKa = 3.4 PNAALVRR246 pKa = 11.84 IQSLSYY252 pKa = 9.85 TGPIKK257 pKa = 10.23 MKK259 pKa = 10.31 QSNGTLTVIPEE270 pKa = 4.11 RR271 pKa = 11.84 NVYY274 pKa = 8.23 PTILLTEE281 pKa = 4.75 KK282 pKa = 10.6 GSHH285 pKa = 6.6 PPGATKK291 pKa = 9.66 TFRR294 pKa = 11.84 CLSYY298 pKa = 10.17 PATSVNPEE306 pKa = 4.2 YY307 pKa = 10.47 YY308 pKa = 10.6 NSVAPFRR315 pKa = 11.84 STRR318 pKa = 11.84 LNASALLLTNVTKK331 pKa = 10.79 VLNKK335 pKa = 9.91 EE336 pKa = 4.13 GTVEE340 pKa = 3.9 SSRR343 pKa = 11.84 VIFNPDD349 pKa = 2.72 SGRR352 pKa = 11.84 TFMNADD358 pKa = 3.49 VPTVSTSNPEE368 pKa = 3.47 TRR370 pKa = 11.84 YY371 pKa = 10.25 FGALEE376 pKa = 3.95 KK377 pKa = 10.7 GAYY380 pKa = 8.23 TFTAPDD386 pKa = 3.4 QEE388 pKa = 4.64 SLVFTTPYY396 pKa = 8.39 EE397 pKa = 4.25 TVRR400 pKa = 11.84 VNDD403 pKa = 3.81 ALIGHH408 pKa = 7.35 GGGSVPIFVPEE419 pKa = 3.98 RR420 pKa = 11.84 LAEE423 pKa = 4.01 RR424 pKa = 11.84 PVMNLNPRR432 pKa = 11.84 YY433 pKa = 8.66 MNAIILTDD441 pKa = 4.31 LDD443 pKa = 4.03 MTDD446 pKa = 3.83 DD447 pKa = 3.75 TQLALTLDD455 pKa = 3.78 THH457 pKa = 5.87 WEE459 pKa = 3.82 FRR461 pKa = 11.84 TISTLYY467 pKa = 9.4 QLDD470 pKa = 4.11 YY471 pKa = 11.39 SRR473 pKa = 11.84 MPIEE477 pKa = 4.51 TYY479 pKa = 9.85 HH480 pKa = 7.02 AAMLAVMRR488 pKa = 11.84 AGLFYY493 pKa = 10.94 EE494 pKa = 4.59 NNTHH498 pKa = 6.48 QRR500 pKa = 11.84 ILQAVGKK507 pKa = 8.47 GLKK510 pKa = 9.32 FAAPLIPGGNMLQLAHH526 pKa = 6.55 SAAKK530 pKa = 10.11 AVADD534 pKa = 3.6 SRR536 pKa = 11.84 GKK538 pKa = 10.69 KK539 pKa = 8.41 KK540 pKa = 9.19 TPTANSSKK548 pKa = 9.36 RR549 pKa = 11.84 TKK551 pKa = 10.33 DD552 pKa = 3.14 SKK554 pKa = 8.14 QTKK557 pKa = 10.21 GSMKK561 pKa = 10.46 QKK563 pKa = 10.47 GLKK566 pKa = 9.49
Molecular weight: 62.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.162
IPC2_protein 8.244
IPC_protein 8.185
Toseland 8.609
ProMoST 8.697
Dawson 8.99
Bjellqvist 8.873
Wikipedia 9.165
Rodwell 9.077
Grimsley 9.004
Solomon 9.077
Lehninger 9.019
Nozaki 8.843
DTASelect 8.799
Thurlkill 8.858
EMBOSS 9.107
Sillero 9.019
Patrickios 4.368
IPC_peptide 9.063
IPC2_peptide 7.556
IPC2.peptide.svr19 7.606
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1818
566
1252
909.0
100.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.801 ± 0.552
1.87 ± 0.72
5.556 ± 0.232
5.831 ± 0.855
3.19 ± 0.09
7.591 ± 0.756
2.53 ± 0.505
4.51 ± 0.14
5.996 ± 0.469
8.526 ± 0.545
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.475 ± 0.096
3.245 ± 0.725
7.261 ± 0.104
2.915 ± 0.142
6.106 ± 0.433
5.941 ± 0.605
6.766 ± 2.156
5.776 ± 0.409
1.43 ± 0.484
3.685 ± 0.393
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here