Curvibacter phage P26059B
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A384V0X5|A0A384V0X5_9CAUD DNA primase/helicase OS=Curvibacter phage P26059B OX=1983784 GN=P26059B_0030 PE=4 SV=1
MM1 pKa = 6.52 VTITGEE7 pKa = 3.87 AVTLVNLIYY16 pKa = 9.38 GTEE19 pKa = 3.77 EE20 pKa = 5.13 GYY22 pKa = 8.49 TVEE25 pKa = 5.44 DD26 pKa = 3.5 MSEE29 pKa = 4.25 AIALAVDD36 pKa = 3.51 IMGFTDD42 pKa = 3.97 DD43 pKa = 5.45 AKK45 pKa = 11.43 ADD47 pKa = 4.1 LYY49 pKa = 11.68 SLLAEE54 pKa = 4.63 SLTADD59 pKa = 3.49 TKK61 pKa = 11.28 YY62 pKa = 11.13 YY63 pKa = 10.19 LALSIVEE70 pKa = 4.25 DD71 pKa = 4.58 AIIKK75 pKa = 9.32 NKK77 pKa = 9.92 RR78 pKa = 11.84 RR79 pKa = 11.84 FPRR82 pKa = 11.84 GG83 pKa = 3.0
Molecular weight: 9.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.318
IPC2_protein 4.279
IPC_protein 4.151
Toseland 3.961
ProMoST 4.291
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.024
Rodwell 3.986
Grimsley 3.884
Solomon 4.101
Lehninger 4.062
Nozaki 4.24
DTASelect 4.406
Thurlkill 3.999
EMBOSS 4.037
Sillero 4.266
Patrickios 3.592
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.191
Protein with the highest isoelectric point:
>tr|A0A384V7R6|A0A384V7R6_9CAUD Uncharacterized protein OS=Curvibacter phage P26059B OX=1983784 GN=P26059B_0042 PE=4 SV=1
MM1 pKa = 7.18 KK2 pKa = 9.3 HH3 pKa = 5.49 TYY5 pKa = 9.98 KK6 pKa = 9.36 HH7 pKa = 5.95 TKK9 pKa = 8.57 PQPSWLLQGLMIRR22 pKa = 11.84 AGLYY26 pKa = 10.48 DD27 pKa = 4.62 PLPPVAKK34 pKa = 9.88 SHH36 pKa = 6.19 AVPHH40 pKa = 5.96 EE41 pKa = 4.16 MPLITRR47 pKa = 11.84 QLGKK51 pKa = 10.33 SWLQRR56 pKa = 11.84 AWAKK60 pKa = 10.79 AKK62 pKa = 10.41 AAFVYY67 pKa = 10.67 DD68 pKa = 3.93 PARR71 pKa = 11.84 TT72 pKa = 3.49
Molecular weight: 8.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.24
IPC2_protein 9.882
IPC_protein 10.16
Toseland 10.613
ProMoST 10.175
Dawson 10.716
Bjellqvist 10.35
Wikipedia 10.862
Rodwell 11.286
Grimsley 10.774
Solomon 10.76
Lehninger 10.745
Nozaki 10.57
DTASelect 10.35
Thurlkill 10.599
EMBOSS 10.979
Sillero 10.628
Patrickios 11.052
IPC_peptide 10.774
IPC2_peptide 8.931
IPC2.peptide.svr19 8.692
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
12670
42
1577
275.4
30.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.776 ± 0.511
0.781 ± 0.123
5.683 ± 0.237
5.406 ± 0.325
3.37 ± 0.157
7.845 ± 0.279
1.602 ± 0.154
4.262 ± 0.171
5.659 ± 0.38
8.319 ± 0.264
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.502 ± 0.171
4.451 ± 0.226
4.191 ± 0.168
4.262 ± 0.233
5.257 ± 0.305
6.085 ± 0.45
6.377 ± 0.286
7.498 ± 0.238
1.279 ± 0.127
3.394 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here