Amino acid dipepetide frequency for Curvibacter phage P26059B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.813AlaAla: 16.813 ± 1.969
0.71AlaCys: 0.71 ± 0.199
6.867AlaAsp: 6.867 ± 0.923
7.025AlaGlu: 7.025 ± 0.917
3.315AlaPhe: 3.315 ± 0.425
9.156AlaGly: 9.156 ± 0.934
1.579AlaHis: 1.579 ± 0.392
5.525AlaIle: 5.525 ± 0.791
8.051AlaLys: 8.051 ± 0.876
8.604AlaLeu: 8.604 ± 0.785
3.078AlaMet: 3.078 ± 0.694
5.683AlaAsn: 5.683 ± 0.558
3.473AlaPro: 3.473 ± 0.552
4.262AlaGln: 4.262 ± 0.788
5.92AlaArg: 5.92 ± 0.68
7.42AlaSer: 7.42 ± 0.99
6.472AlaThr: 6.472 ± 0.955
8.367AlaVal: 8.367 ± 1.299
1.026AlaTrp: 1.026 ± 0.227
4.42AlaTyr: 4.42 ± 0.617
0.0AlaXaa: 0.0 ± 0.0
Cys
1.263CysAla: 1.263 ± 0.404
0.079CysCys: 0.079 ± 0.085
0.237CysAsp: 0.237 ± 0.132
0.079CysGlu: 0.079 ± 0.07
0.631CysPhe: 0.631 ± 0.212
0.553CysGly: 0.553 ± 0.26
0.079CysHis: 0.079 ± 0.079
0.395CysIle: 0.395 ± 0.227
0.553CysLys: 0.553 ± 0.196
1.026CysLeu: 1.026 ± 0.364
0.395CysMet: 0.395 ± 0.158
0.237CysAsn: 0.237 ± 0.149
0.158CysPro: 0.158 ± 0.117
0.395CysGln: 0.395 ± 0.177
0.079CysArg: 0.079 ± 0.082
0.395CysSer: 0.395 ± 0.187
0.395CysThr: 0.395 ± 0.164
0.474CysVal: 0.474 ± 0.206
0.079CysTrp: 0.079 ± 0.075
0.316CysTyr: 0.316 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
6.394AspAla: 6.394 ± 0.542
0.868AspCys: 0.868 ± 0.319
3.473AspAsp: 3.473 ± 0.569
2.684AspGlu: 2.684 ± 0.543
2.447AspPhe: 2.447 ± 0.319
3.315AspGly: 3.315 ± 0.573
1.105AspHis: 1.105 ± 0.333
3.078AspIle: 3.078 ± 0.563
3.947AspLys: 3.947 ± 0.565
4.815AspLeu: 4.815 ± 0.683
1.263AspMet: 1.263 ± 0.329
1.894AspAsn: 1.894 ± 0.311
3.789AspPro: 3.789 ± 0.438
1.894AspGln: 1.894 ± 0.531
2.052AspArg: 2.052 ± 0.439
3.552AspSer: 3.552 ± 0.544
3.078AspThr: 3.078 ± 0.397
4.42AspVal: 4.42 ± 0.513
0.71AspTrp: 0.71 ± 0.249
2.052AspTyr: 2.052 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
6.788GluAla: 6.788 ± 0.814
0.316GluCys: 0.316 ± 0.144
2.999GluAsp: 2.999 ± 0.519
3.315GluGlu: 3.315 ± 0.575
2.052GluPhe: 2.052 ± 0.476
2.999GluGly: 2.999 ± 0.479
0.789GluHis: 0.789 ± 0.265
2.447GluIle: 2.447 ± 0.241
1.973GluLys: 1.973 ± 0.417
4.578GluLeu: 4.578 ± 0.649
0.947GluMet: 0.947 ± 0.232
2.368GluAsn: 2.368 ± 0.444
1.658GluPro: 1.658 ± 0.542
3.236GluGln: 3.236 ± 0.483
4.183GluArg: 4.183 ± 0.619
2.605GluSer: 2.605 ± 0.447
2.921GluThr: 2.921 ± 0.56
4.657GluVal: 4.657 ± 0.669
1.342GluTrp: 1.342 ± 0.384
1.894GluTyr: 1.894 ± 0.554
0.0GluXaa: 0.0 ± 0.0
Phe
3.315PheAla: 3.315 ± 0.59
0.395PheCys: 0.395 ± 0.208
2.763PheAsp: 2.763 ± 0.558
2.052PheGlu: 2.052 ± 0.32
1.5PhePhe: 1.5 ± 0.247
2.999PheGly: 2.999 ± 0.487
0.237PheHis: 0.237 ± 0.114
1.184PheIle: 1.184 ± 0.237
1.737PheLys: 1.737 ± 0.405
2.052PheLeu: 2.052 ± 0.376
0.868PheMet: 0.868 ± 0.301
2.368PheAsn: 2.368 ± 0.41
1.184PhePro: 1.184 ± 0.315
1.342PheGln: 1.342 ± 0.311
1.263PheArg: 1.263 ± 0.326
2.052PheSer: 2.052 ± 0.419
2.368PheThr: 2.368 ± 0.36
2.684PheVal: 2.684 ± 0.447
0.395PheTrp: 0.395 ± 0.166
0.947PheTyr: 0.947 ± 0.236
0.0PheXaa: 0.0 ± 0.0
Gly
8.209GlyAla: 8.209 ± 1.208
0.71GlyCys: 0.71 ± 0.233
3.473GlyAsp: 3.473 ± 0.465
3.947GlyGlu: 3.947 ± 0.755
2.842GlyPhe: 2.842 ± 0.487
7.656GlyGly: 7.656 ± 0.746
0.789GlyHis: 0.789 ± 0.346
2.921GlyIle: 2.921 ± 0.375
4.973GlyLys: 4.973 ± 0.676
6.551GlyLeu: 6.551 ± 0.641
1.815GlyMet: 1.815 ± 0.469
3.71GlyAsn: 3.71 ± 0.445
2.21GlyPro: 2.21 ± 0.445
3.631GlyGln: 3.631 ± 0.553
3.631GlyArg: 3.631 ± 0.682
5.131GlySer: 5.131 ± 1.016
6.078GlyThr: 6.078 ± 0.703
5.92GlyVal: 5.92 ± 0.78
1.184GlyTrp: 1.184 ± 0.313
2.999GlyTyr: 2.999 ± 0.445
0.0GlyXaa: 0.0 ± 0.0
His
1.815HisAla: 1.815 ± 0.392
0.0HisCys: 0.0 ± 0.0
0.947HisAsp: 0.947 ± 0.28
0.947HisGlu: 0.947 ± 0.324
0.631HisPhe: 0.631 ± 0.21
1.184HisGly: 1.184 ± 0.358
0.158HisHis: 0.158 ± 0.102
0.631HisIle: 0.631 ± 0.201
0.947HisLys: 0.947 ± 0.266
1.737HisLeu: 1.737 ± 0.44
0.395HisMet: 0.395 ± 0.168
0.553HisAsn: 0.553 ± 0.235
0.71HisPro: 0.71 ± 0.21
0.553HisGln: 0.553 ± 0.206
1.026HisArg: 1.026 ± 0.469
0.789HisSer: 0.789 ± 0.24
1.184HisThr: 1.184 ± 0.338
1.105HisVal: 1.105 ± 0.245
0.237HisTrp: 0.237 ± 0.14
0.474HisTyr: 0.474 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
4.815IleAla: 4.815 ± 0.634
0.395IleCys: 0.395 ± 0.19
2.289IleAsp: 2.289 ± 0.422
2.921IleGlu: 2.921 ± 0.447
0.947IlePhe: 0.947 ± 0.287
2.763IleGly: 2.763 ± 0.458
1.184IleHis: 1.184 ± 0.302
2.052IleIle: 2.052 ± 0.39
2.447IleLys: 2.447 ± 0.428
2.842IleLeu: 2.842 ± 0.449
1.5IleMet: 1.5 ± 0.408
1.737IleAsn: 1.737 ± 0.369
1.894IlePro: 1.894 ± 0.445
1.737IleGln: 1.737 ± 0.298
2.052IleArg: 2.052 ± 0.328
2.447IleSer: 2.447 ± 0.484
3.71IleThr: 3.71 ± 0.539
3.789IleVal: 3.789 ± 0.581
0.158IleTrp: 0.158 ± 0.123
0.947IleTyr: 0.947 ± 0.237
0.0IleXaa: 0.0 ± 0.0
Lys
7.735LysAla: 7.735 ± 0.88
0.631LysCys: 0.631 ± 0.224
2.526LysAsp: 2.526 ± 0.453
3.552LysGlu: 3.552 ± 0.606
1.973LysPhe: 1.973 ± 0.403
3.473LysGly: 3.473 ± 0.629
1.026LysHis: 1.026 ± 0.32
2.368LysIle: 2.368 ± 0.382
2.999LysLys: 2.999 ± 0.502
4.815LysLeu: 4.815 ± 0.497
1.263LysMet: 1.263 ± 0.24
2.052LysAsn: 2.052 ± 0.38
2.684LysPro: 2.684 ± 0.438
2.526LysGln: 2.526 ± 0.383
3.868LysArg: 3.868 ± 0.528
2.605LysSer: 2.605 ± 0.398
3.157LysThr: 3.157 ± 0.66
4.815LysVal: 4.815 ± 0.796
0.947LysTrp: 0.947 ± 0.321
1.579LysTyr: 1.579 ± 0.445
0.0LysXaa: 0.0 ± 0.0
Leu
9.709LeuAla: 9.709 ± 0.995
0.868LeuCys: 0.868 ± 0.282
4.973LeuAsp: 4.973 ± 0.556
4.578LeuGlu: 4.578 ± 0.544
1.658LeuPhe: 1.658 ± 0.284
6.157LeuGly: 6.157 ± 0.609
2.052LeuHis: 2.052 ± 0.372
3.236LeuIle: 3.236 ± 0.695
4.105LeuLys: 4.105 ± 0.548
7.183LeuLeu: 7.183 ± 0.702
2.289LeuMet: 2.289 ± 0.39
2.921LeuAsn: 2.921 ± 0.395
4.183LeuPro: 4.183 ± 0.548
4.262LeuGln: 4.262 ± 0.628
4.183LeuArg: 4.183 ± 0.579
6.236LeuSer: 6.236 ± 0.632
4.736LeuThr: 4.736 ± 0.51
6.551LeuVal: 6.551 ± 0.621
0.868LeuTrp: 0.868 ± 0.216
2.447LeuTyr: 2.447 ± 0.438
0.0LeuXaa: 0.0 ± 0.0
Met
2.763MetAla: 2.763 ± 0.519
0.079MetCys: 0.079 ± 0.07
1.263MetAsp: 1.263 ± 0.359
1.579MetGlu: 1.579 ± 0.357
0.553MetPhe: 0.553 ± 0.193
2.763MetGly: 2.763 ± 0.469
0.316MetHis: 0.316 ± 0.15
0.71MetIle: 0.71 ± 0.214
1.5MetLys: 1.5 ± 0.317
2.684MetLeu: 2.684 ± 0.344
0.474MetMet: 0.474 ± 0.15
0.868MetAsn: 0.868 ± 0.25
1.026MetPro: 1.026 ± 0.271
0.947MetGln: 0.947 ± 0.259
1.815MetArg: 1.815 ± 0.393
1.894MetSer: 1.894 ± 0.385
1.894MetThr: 1.894 ± 0.424
0.947MetVal: 0.947 ± 0.269
0.079MetTrp: 0.079 ± 0.07
0.868MetTyr: 0.868 ± 0.243
0.0MetXaa: 0.0 ± 0.0
Asn
4.657AsnAla: 4.657 ± 0.631
0.474AsnCys: 0.474 ± 0.225
2.447AsnAsp: 2.447 ± 0.41
1.342AsnGlu: 1.342 ± 0.379
1.342AsnPhe: 1.342 ± 0.309
4.815AsnGly: 4.815 ± 0.769
0.395AsnHis: 0.395 ± 0.139
1.658AsnIle: 1.658 ± 0.287
2.605AsnLys: 2.605 ± 0.397
2.763AsnLeu: 2.763 ± 0.565
1.184AsnMet: 1.184 ± 0.316
2.605AsnAsn: 2.605 ± 0.5
2.368AsnPro: 2.368 ± 0.424
1.5AsnGln: 1.5 ± 0.291
2.684AsnArg: 2.684 ± 0.514
2.131AsnSer: 2.131 ± 0.528
3.078AsnThr: 3.078 ± 0.549
3.868AsnVal: 3.868 ± 0.641
0.631AsnTrp: 0.631 ± 0.285
1.973AsnTyr: 1.973 ± 0.449
0.0AsnXaa: 0.0 ± 0.0
Pro
4.499ProAla: 4.499 ± 0.712
0.395ProCys: 0.395 ± 0.158
3.157ProAsp: 3.157 ± 0.512
2.684ProGlu: 2.684 ± 0.643
1.421ProPhe: 1.421 ± 0.366
2.999ProGly: 2.999 ± 0.517
0.474ProHis: 0.474 ± 0.2
1.342ProIle: 1.342 ± 0.357
2.289ProLys: 2.289 ± 0.385
3.947ProLeu: 3.947 ± 0.56
0.868ProMet: 0.868 ± 0.287
1.5ProAsn: 1.5 ± 0.288
1.421ProPro: 1.421 ± 0.43
1.421ProGln: 1.421 ± 0.369
1.579ProArg: 1.579 ± 0.448
2.447ProSer: 2.447 ± 0.397
3.473ProThr: 3.473 ± 0.496
3.868ProVal: 3.868 ± 0.439
0.631ProTrp: 0.631 ± 0.202
1.5ProTyr: 1.5 ± 0.295
0.0ProXaa: 0.0 ± 0.0
Gln
6.157GlnAla: 6.157 ± 0.999
0.079GlnCys: 0.079 ± 0.078
1.737GlnAsp: 1.737 ± 0.338
2.447GlnGlu: 2.447 ± 0.514
1.184GlnPhe: 1.184 ± 0.287
3.394GlnGly: 3.394 ± 0.434
0.789GlnHis: 0.789 ± 0.309
2.131GlnIle: 2.131 ± 0.404
1.815GlnLys: 1.815 ± 0.448
3.236GlnLeu: 3.236 ± 0.489
1.184GlnMet: 1.184 ± 0.323
2.131GlnAsn: 2.131 ± 0.511
1.342GlnPro: 1.342 ± 0.375
2.21GlnGln: 2.21 ± 0.558
2.842GlnArg: 2.842 ± 0.473
2.999GlnSer: 2.999 ± 0.69
1.894GlnThr: 1.894 ± 0.378
3.157GlnVal: 3.157 ± 0.516
0.631GlnTrp: 0.631 ± 0.197
1.263GlnTyr: 1.263 ± 0.236
0.0GlnXaa: 0.0 ± 0.0
Arg
6.472ArgAla: 6.472 ± 1.028
0.553ArgCys: 0.553 ± 0.318
2.763ArgAsp: 2.763 ± 0.426
2.921ArgGlu: 2.921 ± 0.621
2.131ArgPhe: 2.131 ± 0.324
2.684ArgGly: 2.684 ± 0.535
1.105ArgHis: 1.105 ± 0.332
2.21ArgIle: 2.21 ± 0.462
2.999ArgLys: 2.999 ± 0.424
5.052ArgLeu: 5.052 ± 0.798
1.421ArgMet: 1.421 ± 0.392
2.368ArgAsn: 2.368 ± 0.512
1.815ArgPro: 1.815 ± 0.38
2.368ArgGln: 2.368 ± 0.462
2.842ArgArg: 2.842 ± 0.482
3.236ArgSer: 3.236 ± 0.475
3.315ArgThr: 3.315 ± 0.442
2.605ArgVal: 2.605 ± 0.464
0.868ArgTrp: 0.868 ± 0.324
2.842ArgTyr: 2.842 ± 0.495
0.0ArgXaa: 0.0 ± 0.0
Ser
6.394SerAla: 6.394 ± 1.132
0.237SerCys: 0.237 ± 0.177
3.552SerAsp: 3.552 ± 0.639
2.447SerGlu: 2.447 ± 0.424
2.289SerPhe: 2.289 ± 0.33
5.446SerGly: 5.446 ± 0.775
1.184SerHis: 1.184 ± 0.288
2.842SerIle: 2.842 ± 0.61
3.236SerLys: 3.236 ± 0.487
5.288SerLeu: 5.288 ± 0.72
1.5SerMet: 1.5 ± 0.348
2.684SerAsn: 2.684 ± 0.586
2.684SerPro: 2.684 ± 0.448
2.447SerGln: 2.447 ± 0.472
3.947SerArg: 3.947 ± 0.578
3.473SerSer: 3.473 ± 0.851
3.789SerThr: 3.789 ± 0.661
4.657SerVal: 4.657 ± 0.687
0.868SerTrp: 0.868 ± 0.317
1.894SerTyr: 1.894 ± 0.423
0.0SerXaa: 0.0 ± 0.0
Thr
6.946ThrAla: 6.946 ± 0.739
0.158ThrCys: 0.158 ± 0.116
4.262ThrAsp: 4.262 ± 0.65
3.236ThrGlu: 3.236 ± 0.644
2.842ThrPhe: 2.842 ± 0.601
6.315ThrGly: 6.315 ± 0.637
0.947ThrHis: 0.947 ± 0.215
2.842ThrIle: 2.842 ± 0.453
3.394ThrLys: 3.394 ± 0.428
5.131ThrLeu: 5.131 ± 0.69
1.184ThrMet: 1.184 ± 0.273
2.605ThrAsn: 2.605 ± 0.412
3.71ThrPro: 3.71 ± 0.608
3.078ThrGln: 3.078 ± 0.559
2.052ThrArg: 2.052 ± 0.465
3.868ThrSer: 3.868 ± 0.561
3.631ThrThr: 3.631 ± 0.895
5.446ThrVal: 5.446 ± 0.815
0.631ThrTrp: 0.631 ± 0.204
1.5ThrTyr: 1.5 ± 0.313
0.0ThrXaa: 0.0 ± 0.0
Val
7.183ValAla: 7.183 ± 0.782
0.553ValCys: 0.553 ± 0.243
3.789ValAsp: 3.789 ± 0.665
4.341ValGlu: 4.341 ± 0.54
2.131ValPhe: 2.131 ± 0.288
5.683ValGly: 5.683 ± 0.698
1.105ValHis: 1.105 ± 0.332
2.605ValIle: 2.605 ± 0.493
4.736ValLys: 4.736 ± 0.68
6.551ValLeu: 6.551 ± 0.747
2.131ValMet: 2.131 ± 0.349
4.499ValAsn: 4.499 ± 0.544
3.868ValPro: 3.868 ± 0.6
3.078ValGln: 3.078 ± 0.41
4.42ValArg: 4.42 ± 0.421
4.815ValSer: 4.815 ± 1.008
5.288ValThr: 5.288 ± 0.711
4.578ValVal: 4.578 ± 0.477
1.421ValTrp: 1.421 ± 0.358
2.21ValTyr: 2.21 ± 0.36
0.0ValXaa: 0.0 ± 0.0
Trp
1.894TrpAla: 1.894 ± 0.323
0.158TrpCys: 0.158 ± 0.099
0.947TrpAsp: 0.947 ± 0.249
0.789TrpGlu: 0.789 ± 0.256
0.789TrpPhe: 0.789 ± 0.246
0.947TrpGly: 0.947 ± 0.202
0.079TrpHis: 0.079 ± 0.081
0.631TrpIle: 0.631 ± 0.277
0.631TrpLys: 0.631 ± 0.26
1.342TrpLeu: 1.342 ± 0.376
0.474TrpMet: 0.474 ± 0.181
0.316TrpAsn: 0.316 ± 0.161
0.474TrpPro: 0.474 ± 0.263
0.789TrpGln: 0.789 ± 0.341
0.474TrpArg: 0.474 ± 0.18
0.474TrpSer: 0.474 ± 0.25
0.395TrpThr: 0.395 ± 0.205
0.868TrpVal: 0.868 ± 0.295
0.0TrpTrp: 0.0 ± 0.0
0.316TrpTyr: 0.316 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.947TyrAla: 3.947 ± 0.398
0.158TyrCys: 0.158 ± 0.108
2.368TyrAsp: 2.368 ± 0.458
1.184TyrGlu: 1.184 ± 0.284
1.026TyrPhe: 1.026 ± 0.24
3.157TyrGly: 3.157 ± 0.511
0.631TyrHis: 0.631 ± 0.28
1.815TyrIle: 1.815 ± 0.335
1.658TyrLys: 1.658 ± 0.399
2.999TyrLeu: 2.999 ± 0.607
0.71TyrMet: 0.71 ± 0.174
1.421TyrAsn: 1.421 ± 0.27
1.263TyrPro: 1.263 ± 0.285
0.947TyrGln: 0.947 ± 0.318
1.658TyrArg: 1.658 ± 0.429
2.289TyrSer: 2.289 ± 0.333
2.921TyrThr: 2.921 ± 0.503
2.21TyrVal: 2.21 ± 0.349
0.079TyrTrp: 0.079 ± 0.076
1.5TyrTyr: 1.5 ± 0.36
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (12670 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski