Streptococcus satellite phage Javan294

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZIK6|A0A4D5ZIK6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan294 OX=2558613 GN=JavanS294_0002 PE=4 SV=1
MM1 pKa = 7.27NRR3 pKa = 11.84LKK5 pKa = 10.58EE6 pKa = 3.96LRR8 pKa = 11.84QEE10 pKa = 4.17KK11 pKa = 9.72NAKK14 pKa = 9.67QEE16 pKa = 4.08DD17 pKa = 4.01LAEE20 pKa = 4.27VASVSAMTISRR31 pKa = 11.84WEE33 pKa = 3.97NGEE36 pKa = 4.19SQIKK40 pKa = 9.07PDD42 pKa = 3.87KK43 pKa = 10.83AQTLADD49 pKa = 3.72YY50 pKa = 10.58FGVSVAYY57 pKa = 10.62LLGYY61 pKa = 10.32EE62 pKa = 4.36EE63 pKa = 6.26ADD65 pKa = 3.41TLEE68 pKa = 4.86NILKK72 pKa = 10.01EE73 pKa = 4.01AEE75 pKa = 4.26EE76 pKa = 4.09YY77 pKa = 11.0LGLTEE82 pKa = 5.41EE83 pKa = 5.14DD84 pKa = 5.44LLAQDD89 pKa = 3.96YY90 pKa = 8.55TSKK93 pKa = 10.76IKK95 pKa = 10.38IALTEE100 pKa = 3.92MSNIPILKK108 pKa = 10.31NEE110 pKa = 4.54IIYY113 pKa = 10.99NLDD116 pKa = 3.27FLSFDD121 pKa = 3.36NLKK124 pKa = 10.72AINQIVRR131 pKa = 11.84DD132 pKa = 4.06LPKK135 pKa = 10.58KK136 pKa = 10.45EE137 pKa = 4.11DD138 pKa = 3.53TDD140 pKa = 3.66NN141 pKa = 3.58

Molecular weight: 16.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNL3|A0A4D5ZNL3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan294 OX=2558613 GN=JavanS294_0009 PE=4 SV=1
MM1 pKa = 7.13TRR3 pKa = 11.84YY4 pKa = 9.33LKK6 pKa = 10.8HH7 pKa = 5.84PALRR11 pKa = 11.84NLKK14 pKa = 9.76IGQEE18 pKa = 4.28SNIKK22 pKa = 10.08DD23 pKa = 3.17RR24 pKa = 11.84RR25 pKa = 11.84LTIEE29 pKa = 3.55EE30 pKa = 3.98VEE32 pKa = 3.92RR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 10.01AQKK38 pKa = 10.32RR39 pKa = 11.84LQKK42 pKa = 9.99KK43 pKa = 9.6RR44 pKa = 11.84KK45 pKa = 8.35RR46 pKa = 3.66

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

2629

46

573

154.6

17.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.352 ± 0.632

0.266 ± 0.083

5.896 ± 0.419

8.254 ± 0.893

3.652 ± 0.378

4.869 ± 0.488

1.598 ± 0.244

6.2 ± 0.442

9.89 ± 0.528

10.917 ± 0.631

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.244 ± 0.272

5.021 ± 0.482

3.081 ± 0.573

5.173 ± 0.397

5.706 ± 0.601

4.412 ± 0.293

6.314 ± 0.663

4.603 ± 0.418

0.989 ± 0.148

4.564 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski