Planctomycetes bacterium K23_9

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5858 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517NUL2|A0A517NUL2_9BACT Putative HTH-type transcriptional regulator YybR OS=Planctomycetes bacterium K23_9 OX=1930275 GN=yybR_1 PE=4 SV=1
MM1 pKa = 6.4MTIDD5 pKa = 5.25ALIDD9 pKa = 3.59LLNEE13 pKa = 3.56YY14 pKa = 10.08RR15 pKa = 11.84EE16 pKa = 4.17QHH18 pKa = 6.15GPDD21 pKa = 3.1AEE23 pKa = 4.32VRR25 pKa = 11.84LMTQEE30 pKa = 3.41NWPFEE35 pKa = 3.92NRR37 pKa = 11.84IAGITSGAEE46 pKa = 3.57MNEE49 pKa = 3.83ASEE52 pKa = 4.58EE53 pKa = 4.12DD54 pKa = 3.48PSEE57 pKa = 4.28YY58 pKa = 10.54FDD60 pKa = 4.65DD61 pKa = 4.13QDD63 pKa = 3.66VAEE66 pKa = 4.68DD67 pKa = 4.25AIVYY71 pKa = 9.53IVEE74 pKa = 4.89GGQICYY80 pKa = 10.07GSKK83 pKa = 10.35RR84 pKa = 11.84AWEE87 pKa = 4.2TCRR90 pKa = 11.84DD91 pKa = 3.78CC92 pKa = 6.52

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517P1R3|A0A517P1R3_9BACT Type II secretion system protein E OS=Planctomycetes bacterium K23_9 OX=1930275 GN=gspE_3 PE=3 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84RR3 pKa = 11.84SNNAKK8 pKa = 9.71SLYY11 pKa = 10.17SQDD14 pKa = 3.0HH15 pKa = 5.46SKK17 pKa = 10.15IRR19 pKa = 11.84SLRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84AVGRR28 pKa = 11.84FAEE31 pKa = 4.49CTPAEE36 pKa = 4.17WLFATGPLKK45 pKa = 10.57PIAGSEE51 pKa = 4.28SEE53 pKa = 4.79LDD55 pKa = 3.06NHH57 pKa = 5.59YY58 pKa = 10.63QCRR61 pKa = 11.84ALFLRR66 pKa = 11.84WGTQNIHH73 pKa = 6.84KK74 pKa = 10.35SRR76 pKa = 11.84LIDD79 pKa = 3.37RR80 pKa = 11.84VLKK83 pKa = 8.68THH85 pKa = 6.25SLRR88 pKa = 11.84MSLCCCQHH96 pKa = 5.62FQRR99 pKa = 11.84EE100 pKa = 4.17RR101 pKa = 3.25

Molecular weight: 11.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5858

0

5858

2345483

29

11999

400.4

43.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.668 ± 0.033

1.105 ± 0.017

6.741 ± 0.044

5.565 ± 0.033

3.697 ± 0.019

7.597 ± 0.051

2.138 ± 0.023

5.226 ± 0.019

3.928 ± 0.045

9.276 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.022

3.478 ± 0.032

4.929 ± 0.029

4.263 ± 0.025

6.243 ± 0.05

7.061 ± 0.031

5.855 ± 0.064

7.241 ± 0.032

1.401 ± 0.016

2.317 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski