Neptunomonas marina
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3482 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A437Q3U1|A0A437Q3U1_9GAMM Histidine kinase OS=Neptunomonas marina OX=1815562 GN=EOE65_17370 PE=4 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 AHH4 pKa = 7.03 TYY6 pKa = 10.29 SGYY9 pKa = 8.87 LTLLFAVCAVFLFPSKK25 pKa = 10.79 ASAYY29 pKa = 10.83 LMGDD33 pKa = 3.98 GGCTTDD39 pKa = 3.3 SVSITGISEE48 pKa = 4.32 TSSGTPIYY56 pKa = 10.47 SGSLSATSCAGLFQGINDD74 pKa = 4.21 GPANNPDD81 pKa = 3.85 PNIGEE86 pKa = 4.43 LEE88 pKa = 4.2 DD89 pKa = 4.3 GFLNGEE95 pKa = 4.18 PVKK98 pKa = 10.15 TGPRR102 pKa = 11.84 TEE104 pKa = 5.41 DD105 pKa = 3.29 GLDD108 pKa = 3.41 PLTFIDD114 pKa = 4.03 STEE117 pKa = 3.96 LQALSGDD124 pKa = 4.6 GIFDD128 pKa = 3.93 DD129 pKa = 5.23 PGWIHH134 pKa = 6.79 LAEE137 pKa = 5.04 IDD139 pKa = 3.66 ADD141 pKa = 3.7 SGTRR145 pKa = 11.84 YY146 pKa = 10.51 SFLDD150 pKa = 3.57 TLDD153 pKa = 3.94 LASVLDD159 pKa = 3.86 VSLVCNLGPPNDD171 pKa = 4.32 CKK173 pKa = 11.19 GGTWNLAVDD182 pKa = 4.48 PAAIAAVQAILGPNSFDD199 pKa = 3.07 HH200 pKa = 6.49 LAFVFKK206 pKa = 10.83 SSTAVSIYY214 pKa = 10.93 DD215 pKa = 3.57 FDD217 pKa = 6.51 FIDD220 pKa = 4.48 LAPAIGGGFNFATAYY235 pKa = 9.23 TFTGNWNMDD244 pKa = 3.58 DD245 pKa = 3.91 FQNPNNNNAQGYY257 pKa = 6.78 SHH259 pKa = 7.11 ISFWARR265 pKa = 11.84 DD266 pKa = 3.58 PADD269 pKa = 3.7 AADD272 pKa = 3.96 GTEE275 pKa = 3.97 IPTPGTLVLFGLGLLLLLFRR295 pKa = 11.84 LRR297 pKa = 11.84 LGRR300 pKa = 11.84 AA301 pKa = 3.18
Molecular weight: 31.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.617
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.681
Grimsley 3.528
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.279
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.986
Patrickios 1.189
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|A0A437QB87|A0A437QB87_9GAMM Probable nicotinate-nucleotide adenylyltransferase OS=Neptunomonas marina OX=1815562 GN=nadD PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.31 SGRR28 pKa = 11.84 AVINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.7 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3482
0
3482
1104039
23
2272
317.1
35.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.116 ± 0.051
1.026 ± 0.015
5.532 ± 0.033
6.395 ± 0.038
3.836 ± 0.031
7.045 ± 0.038
2.293 ± 0.023
5.715 ± 0.031
4.279 ± 0.034
10.742 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.53 ± 0.018
3.447 ± 0.026
4.264 ± 0.027
4.692 ± 0.034
5.406 ± 0.033
6.245 ± 0.029
5.233 ± 0.024
7.196 ± 0.036
1.237 ± 0.016
2.771 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here