CRESS virus sp. ctWOo3
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2WC86|A0A5Q2WC86_9VIRU Putative capsid protein OS=CRESS virus sp. ctWOo3 OX=2656678 PE=4 SV=1
MM1 pKa = 7.88 SDD3 pKa = 3.3 RR4 pKa = 11.84 LDD6 pKa = 3.46 TVSISRR12 pKa = 11.84 ACARR16 pKa = 11.84 WLIDD20 pKa = 3.38 YY21 pKa = 8.74 GRR23 pKa = 11.84 QLVNDD28 pKa = 5.65 GYY30 pKa = 10.59 GTDD33 pKa = 3.92 YY34 pKa = 11.67 DD35 pKa = 4.33 PFNPVADD42 pKa = 5.05 LEE44 pKa = 4.46 PEE46 pKa = 4.15 GAGFIDD52 pKa = 6.26 DD53 pKa = 3.87 IPMEE57 pKa = 4.55 NGEE60 pKa = 3.99 MWRR63 pKa = 11.84 FMNALINFARR73 pKa = 11.84 TVAGVLARR81 pKa = 11.84 NMNDD85 pKa = 3.69 PISPLTVSDD94 pKa = 5.45 LFLEE98 pKa = 4.51 DD99 pKa = 3.4 EE100 pKa = 4.57 KK101 pKa = 11.51 EE102 pKa = 3.86 II103 pKa = 4.59
Molecular weight: 11.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.268
IPC2_protein 3.973
IPC_protein 3.923
Toseland 3.706
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.63
Solomon 3.91
Lehninger 3.859
Nozaki 4.037
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.05
Patrickios 3.719
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.968
Protein with the highest isoelectric point:
>tr|A0A5Q2WC86|A0A5Q2WC86_9VIRU Putative capsid protein OS=CRESS virus sp. ctWOo3 OX=2656678 PE=4 SV=1
MM1 pKa = 7.43 RR2 pKa = 11.84 RR3 pKa = 11.84 KK4 pKa = 10.42 LFVSPFAKK12 pKa = 9.89 PQGAVRR18 pKa = 11.84 PNRR21 pKa = 11.84 GQANKK26 pKa = 10.36 KK27 pKa = 9.82 SYY29 pKa = 9.57 TYY31 pKa = 10.73 RR32 pKa = 11.84 KK33 pKa = 6.47 NTRR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.87 FSPGGFDD45 pKa = 3.19 TDD47 pKa = 3.02 KK48 pKa = 10.58 MLRR51 pKa = 11.84 NHH53 pKa = 7.01 IDD55 pKa = 3.21 NVTKK59 pKa = 10.64 KK60 pKa = 10.7 SIEE63 pKa = 4.11 RR64 pKa = 11.84 QKK66 pKa = 11.03 KK67 pKa = 8.73 PSFDD71 pKa = 3.35 SVAPFVEE78 pKa = 4.7 EE79 pKa = 4.01 KK80 pKa = 10.13 RR81 pKa = 11.84 RR82 pKa = 11.84 KK83 pKa = 9.21 RR84 pKa = 11.84 DD85 pKa = 3.48 FKK87 pKa = 10.84 IRR89 pKa = 11.84 TSVGTSFHH97 pKa = 6.86 VGPIPPPANGTRR109 pKa = 11.84 QDD111 pKa = 4.41 DD112 pKa = 3.96 SSDD115 pKa = 3.55 DD116 pKa = 3.09 TKK118 pKa = 10.64 MIHH121 pKa = 5.91 KK122 pKa = 10.31 VEE124 pKa = 4.25 VKK126 pKa = 10.23 SIVMGSDD133 pKa = 3.47 PSQEE137 pKa = 3.68 FRR139 pKa = 11.84 RR140 pKa = 11.84 KK141 pKa = 9.71 IEE143 pKa = 3.94 TGRR146 pKa = 11.84 PTTKK150 pKa = 10.54 SLLRR154 pKa = 11.84 AAKK157 pKa = 10.34 EE158 pKa = 3.75 NGTTRR163 pKa = 11.84 RR164 pKa = 11.84 IIYY167 pKa = 9.79 DD168 pKa = 3.72 SYY170 pKa = 11.77 NSAITDD176 pKa = 3.61 GLHH179 pKa = 5.66 FTRR182 pKa = 11.84 KK183 pKa = 8.92 QLTVGTGFNQRR194 pKa = 11.84 GFTVFGVPAYY204 pKa = 7.45 MTYY207 pKa = 11.31 GDD209 pKa = 4.31 MLGITDD215 pKa = 3.82 TNLNNTQSAFKK226 pKa = 9.71 MQTLLAGIMSSKK238 pKa = 10.04 TEE240 pKa = 3.73 MLLRR244 pKa = 11.84 NQSAHH249 pKa = 4.88 LRR251 pKa = 11.84 AYY253 pKa = 10.32 VRR255 pKa = 11.84 VHH257 pKa = 6.58 LVSKK261 pKa = 10.73 QCGEE265 pKa = 4.22 VNDD268 pKa = 4.82 PVLDD272 pKa = 3.56 MSNNVSHH279 pKa = 7.16 GDD281 pKa = 3.48 VNAEE285 pKa = 4.77 SIPADD290 pKa = 3.88 LKK292 pKa = 10.17 NKK294 pKa = 9.74 RR295 pKa = 11.84 MPVTYY300 pKa = 10.12 QYY302 pKa = 11.06 TDD304 pKa = 2.81 NRR306 pKa = 11.84 LEE308 pKa = 4.19 TGSTGTVEE316 pKa = 4.8 LFPPSRR322 pKa = 11.84 TLSFDD327 pKa = 3.09 TTTKK331 pKa = 11.03 GNILGQSPYY340 pKa = 10.26 FKK342 pKa = 10.9 EE343 pKa = 3.91 NFKK346 pKa = 10.49 ICATVSQNLGPGDD359 pKa = 4.06 FFRR362 pKa = 11.84 FSHH365 pKa = 4.66 VHH367 pKa = 5.82 HH368 pKa = 6.79 YY369 pKa = 11.2 GSGFDD374 pKa = 3.78 LANLYY379 pKa = 9.58 SEE381 pKa = 5.08 KK382 pKa = 10.84 SLTGPDD388 pKa = 3.39 DD389 pKa = 4.06 PGYY392 pKa = 11.11 AKK394 pKa = 8.32 EE395 pKa = 3.86 TSYY398 pKa = 11.12 FYY400 pKa = 9.86 IVEE403 pKa = 4.39 YY404 pKa = 10.21 KK405 pKa = 10.91 GHH407 pKa = 5.89 ICEE410 pKa = 4.33 ATFNNAGAFEE420 pKa = 4.16 ARR422 pKa = 11.84 IGTAPVLLNAEE433 pKa = 3.87 IRR435 pKa = 11.84 KK436 pKa = 9.11 SVRR439 pKa = 11.84 FVNAPNSGQDD449 pKa = 3.21 QTNSGVAPRR458 pKa = 11.84 CLLRR462 pKa = 11.84 VFEE465 pKa = 4.58 QSSLPNIVEE474 pKa = 3.69 QRR476 pKa = 11.84 RR477 pKa = 11.84 DD478 pKa = 3.69 FNSDD482 pKa = 2.9 LTNWTTVPTPALGLYY497 pKa = 9.19 VIQLSSDD504 pKa = 3.06 RR505 pKa = 11.84 TLRR508 pKa = 11.84 TEE510 pKa = 3.96 VNQAGNSNATDD521 pKa = 3.45 IVPP524 pKa = 3.84
Molecular weight: 58.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.976
IPC2_protein 9.048
IPC_protein 8.99
Toseland 9.721
ProMoST 9.458
Dawson 9.97
Bjellqvist 9.648
Wikipedia 10.145
Rodwell 10.306
Grimsley 10.043
Solomon 9.984
Lehninger 9.955
Nozaki 9.765
DTASelect 9.633
Thurlkill 9.809
EMBOSS 10.145
Sillero 9.882
Patrickios 5.906
IPC_peptide 9.984
IPC2_peptide 8.214
IPC2.peptide.svr19 8.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
932
103
524
310.7
35.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.721 ± 1.184
0.966 ± 0.164
7.296 ± 1.582
5.043 ± 0.719
5.687 ± 0.402
6.116 ± 0.837
1.395 ± 0.592
5.687 ± 1.164
6.223 ± 1.396
7.618 ± 0.885
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.682 ± 0.644
6.438 ± 0.241
5.043 ± 0.5
3.219 ± 0.664
6.545 ± 0.597
7.403 ± 0.807
6.974 ± 1.475
6.438 ± 0.219
0.966 ± 0.613
3.541 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here