Vibrio phage Ceto
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 181 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5BGR2|A0A2H5BGR2_9CAUD Macro domain-containing protein OS=Vibrio phage Ceto OX=2570300 GN=CETO_176 PE=4 SV=1
MM1 pKa = 7.75 ADD3 pKa = 3.23 FWDD6 pKa = 3.36 KK7 pKa = 11.22 HH8 pKa = 4.61 LTKK11 pKa = 9.82 EE12 pKa = 3.97 QQYY15 pKa = 10.59 EE16 pKa = 4.19 IVDD19 pKa = 4.28 SVAGTCDD26 pKa = 3.11 TGCNAEE32 pKa = 4.07 YY33 pKa = 8.82 WQKK36 pKa = 11.08 EE37 pKa = 3.99 FGFSNEE43 pKa = 4.33 VRR45 pKa = 11.84 DD46 pKa = 3.92 EE47 pKa = 4.47 LNWDD51 pKa = 4.02 FPSLAAKK58 pKa = 10.51 FEE60 pKa = 4.34 LFRR63 pKa = 11.84 CEE65 pKa = 4.38 DD66 pKa = 3.84 CMWWGDD72 pKa = 3.46 MGEE75 pKa = 4.25 VGDD78 pKa = 4.54 CNEE81 pKa = 4.77 CGGEE85 pKa = 4.11 LVV87 pKa = 3.95
Molecular weight: 10.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.961
IPC_protein 3.884
Toseland 3.694
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.605
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.77
Sillero 3.999
Patrickios 0.896
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.888
Protein with the highest isoelectric point:
>tr|A0A2H5BGM5|A0A2H5BGM5_9CAUD Uncharacterized protein OS=Vibrio phage Ceto OX=2570300 GN=CETO_165 PE=4 SV=1
MM1 pKa = 8.01 DD2 pKa = 4.66 RR3 pKa = 11.84 RR4 pKa = 11.84 HH5 pKa = 6.23 KK6 pKa = 11.02 LRR8 pKa = 11.84 VNWHH12 pKa = 6.27 RR13 pKa = 11.84 RR14 pKa = 11.84 LKK16 pKa = 10.67 DD17 pKa = 3.35 DD18 pKa = 5.35 EE19 pKa = 4.14 IAIEE23 pKa = 4.07 NGFTTRR29 pKa = 11.84 LFKK32 pKa = 9.91 QTEE35 pKa = 4.38 VYY37 pKa = 9.9 MYY39 pKa = 10.76 DD40 pKa = 3.98 LSWWDD45 pKa = 3.37 YY46 pKa = 10.54 PRR48 pKa = 11.84 RR49 pKa = 11.84 TLEE52 pKa = 4.11 RR53 pKa = 11.84 SWKK56 pKa = 8.9 NQSKK60 pKa = 10.1 KK61 pKa = 9.47 RR62 pKa = 11.84 KK63 pKa = 6.39 QWEE66 pKa = 3.91 RR67 pKa = 3.3
Molecular weight: 8.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.276
IPC2_protein 9.604
IPC_protein 10.101
Toseland 10.555
ProMoST 10.379
Dawson 10.672
Bjellqvist 10.321
Wikipedia 10.833
Rodwell 10.994
Grimsley 10.716
Solomon 10.745
Lehninger 10.716
Nozaki 10.511
DTASelect 10.321
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.584
Patrickios 10.76
IPC_peptide 10.745
IPC2_peptide 8.916
IPC2.peptide.svr19 8.762
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
181
0
181
36038
35
1018
199.1
22.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.678 ± 0.356
1.246 ± 0.096
6.299 ± 0.144
6.92 ± 0.212
4.082 ± 0.146
6.396 ± 0.173
2.048 ± 0.116
6.26 ± 0.15
7.437 ± 0.151
8.585 ± 0.186
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.594 ± 0.093
5.025 ± 0.14
3.535 ± 0.141
3.605 ± 0.127
4.512 ± 0.15
6.008 ± 0.23
6.096 ± 0.188
6.313 ± 0.156
1.423 ± 0.083
3.938 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here