Sporolactobacillus sp. THM19-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Sporolactobacillaceae; Sporolactobacillus; unclassified Sporolactobacillus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4IDS9|A0A4Q4IDS9_9BACL Rhodanese-like domain-containing protein OS=Sporolactobacillus sp. THM19-2 OX=2511171 GN=EWH91_12240 PE=4 SV=1
MM1 pKa = 7.59AKK3 pKa = 10.19YY4 pKa = 10.68VIVDD8 pKa = 3.49KK9 pKa = 9.74DD10 pKa = 3.43TCIACGACGCAAPDD24 pKa = 3.28VFGYY28 pKa = 10.56DD29 pKa = 3.41EE30 pKa = 4.9EE31 pKa = 4.72GLSEE35 pKa = 4.62VLLDD39 pKa = 4.76LNQGTAKK46 pKa = 10.4IPDD49 pKa = 3.81EE50 pKa = 4.53LLDD53 pKa = 4.08DD54 pKa = 4.34VEE56 pKa = 5.67DD57 pKa = 4.3AFEE60 pKa = 4.57GCPSEE65 pKa = 4.41SIKK68 pKa = 10.79MSDD71 pKa = 3.31TPFNGNPLKK80 pKa = 10.98AEE82 pKa = 4.17NEE84 pKa = 4.06ASS86 pKa = 3.32

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4IM44|A0A4Q4IM44_9BACL Uncharacterized protein OS=Sporolactobacillus sp. THM19-2 OX=2511171 GN=EWH91_02455 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.33QPNKK9 pKa = 8.29RR10 pKa = 11.84SRR12 pKa = 11.84KK13 pKa = 7.82RR14 pKa = 11.84VHH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAKK26 pKa = 9.95GRR28 pKa = 11.84KK29 pKa = 8.58ILANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.72GRR39 pKa = 11.84KK40 pKa = 8.69VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2735

0

2735

833227

21

1569

304.7

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.791 ± 0.05

0.867 ± 0.016

5.469 ± 0.04

6.077 ± 0.044

4.367 ± 0.037

7.215 ± 0.042

2.419 ± 0.02

7.171 ± 0.046

6.286 ± 0.046

9.939 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.021

3.767 ± 0.028

3.993 ± 0.024

3.762 ± 0.03

5.508 ± 0.041

6.117 ± 0.034

5.348 ± 0.031

6.854 ± 0.039

1.017 ± 0.018

3.282 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski