Amino acid dipepetide frequency for Sporolactobacillus sp. THM19-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.511AlaAla: 7.511 ± 0.128	0.901AlaCys: 0.901 ± 0.037	4.604AlaAsp: 4.604 ± 0.086	5.007AlaGlu: 5.007 ± 0.076	3.671AlaPhe: 3.671 ± 0.068	6.882AlaGly: 6.882 ± 0.115	1.601AlaHis: 1.601 ± 0.05	5.174AlaIle: 5.174 ± 0.092	4.015AlaLys: 4.015 ± 0.075	8.103AlaLeu: 8.103 ± 0.106	2.049AlaMet: 2.049 ± 0.048	2.22AlaAsn: 2.22 ± 0.06	2.453AlaPro: 2.453 ± 0.063	2.494AlaGln: 2.494 ± 0.061	4.082AlaArg: 4.082 ± 0.069	4.451AlaSer: 4.451 ± 0.081	3.425AlaThr: 3.425 ± 0.074	6.127AlaVal: 6.127 ± 0.099	0.732AlaTrp: 0.732 ± 0.03	2.406AlaTyr: 2.406 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.671CysAla: 0.671 ± 0.03	0.121CysCys: 0.121 ± 0.013	0.474CysAsp: 0.474 ± 0.023	0.414CysGlu: 0.414 ± 0.025	0.382CysPhe: 0.382 ± 0.02	0.893CysGly: 0.893 ± 0.033	0.262CysHis: 0.262 ± 0.017	0.554CysIle: 0.554 ± 0.025	0.299CysLys: 0.299 ± 0.021	0.924CysLeu: 0.924 ± 0.037	0.239CysMet: 0.239 ± 0.02	0.233CysAsn: 0.233 ± 0.018	0.474CysPro: 0.474 ± 0.028	0.235CysGln: 0.235 ± 0.015	0.605CysArg: 0.605 ± 0.028	0.553CysSer: 0.553 ± 0.027	0.516CysThr: 0.516 ± 0.026	0.47CysVal: 0.47 ± 0.024	0.077CysTrp: 0.077 ± 0.01	0.278CysTyr: 0.278 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.868AspAla: 3.868 ± 0.083	0.421AspCys: 0.421 ± 0.023	2.751AspAsp: 2.751 ± 0.07	3.861AspGlu: 3.861 ± 0.079	2.397AspPhe: 2.397 ± 0.055	3.597AspGly: 3.597 ± 0.072	1.668AspHis: 1.668 ± 0.045	3.665AspIle: 3.665 ± 0.071	3.044AspLys: 3.044 ± 0.068	5.493AspLeu: 5.493 ± 0.091	1.393AspMet: 1.393 ± 0.042	1.515AspAsn: 1.515 ± 0.039	2.648AspPro: 2.648 ± 0.058	2.904AspGln: 2.904 ± 0.064	3.486AspArg: 3.486 ± 0.072	2.753AspSer: 2.753 ± 0.052	2.561AspThr: 2.561 ± 0.056	3.815AspVal: 3.815 ± 0.075	0.641AspTrp: 0.641 ± 0.028	2.212AspTyr: 2.212 ± 0.06	0.0AspXaa: 0.0 ± 0.0
Glu	5.199GluAla: 5.199 ± 0.087	0.352GluCys: 0.352 ± 0.02	3.045GluAsp: 3.045 ± 0.068	4.31GluGlu: 4.31 ± 0.091	1.636GluPhe: 1.636 ± 0.047	3.704GluGly: 3.704 ± 0.07	1.457GluHis: 1.457 ± 0.045	4.057GluIle: 4.057 ± 0.074	6.052GluLys: 6.052 ± 0.096	5.533GluLeu: 5.533 ± 0.094	1.849GluMet: 1.849 ± 0.043	2.855GluAsn: 2.855 ± 0.062	1.993GluPro: 1.993 ± 0.053	2.769GluGln: 2.769 ± 0.051	3.485GluArg: 3.485 ± 0.082	3.189GluSer: 3.189 ± 0.057	3.527GluThr: 3.527 ± 0.074	3.57GluVal: 3.57 ± 0.072	0.668GluTrp: 0.668 ± 0.028	1.524GluTyr: 1.524 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	2.973PheAla: 2.973 ± 0.063	0.466PheCys: 0.466 ± 0.022	2.412PheAsp: 2.412 ± 0.051	2.129PheGlu: 2.129 ± 0.053	2.378PhePhe: 2.378 ± 0.062	3.328PheGly: 3.328 ± 0.062	1.147PheHis: 1.147 ± 0.042	3.342PheIle: 3.342 ± 0.071	2.031PheLys: 2.031 ± 0.057	4.481PheLeu: 4.481 ± 0.095	1.032PheMet: 1.032 ± 0.03	1.691PheAsn: 1.691 ± 0.051	1.775PhePro: 1.775 ± 0.052	1.522PheGln: 1.522 ± 0.043	2.171PheArg: 2.171 ± 0.049	3.467PheSer: 3.467 ± 0.069	2.502PheThr: 2.502 ± 0.059	2.784PheVal: 2.784 ± 0.056	0.43PheTrp: 0.43 ± 0.025	1.605PheTyr: 1.605 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	5.21GlyAla: 5.21 ± 0.092	0.743GlyCys: 0.743 ± 0.032	3.29GlyAsp: 3.29 ± 0.066	3.974GlyGlu: 3.974 ± 0.073	3.316GlyPhe: 3.316 ± 0.062	5.015GlyGly: 5.015 ± 0.086	1.737GlyHis: 1.737 ± 0.044	5.679GlyIle: 5.679 ± 0.079	5.279GlyLys: 5.279 ± 0.077	7.161GlyLeu: 7.161 ± 0.096	2.351GlyMet: 2.351 ± 0.058	2.507GlyAsn: 2.507 ± 0.052	1.995GlyPro: 1.995 ± 0.054	2.626GlyGln: 2.626 ± 0.063	4.041GlyArg: 4.041 ± 0.082	4.429GlySer: 4.429 ± 0.076	4.286GlyThr: 4.286 ± 0.077	4.933GlyVal: 4.933 ± 0.082	0.837GlyTrp: 0.837 ± 0.034	2.741GlyTyr: 2.741 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.799HisAla: 1.799 ± 0.046	0.257HisCys: 0.257 ± 0.018	1.227HisAsp: 1.227 ± 0.033	1.327HisGlu: 1.327 ± 0.039	1.289HisPhe: 1.289 ± 0.039	1.566HisGly: 1.566 ± 0.043	0.807HisHis: 0.807 ± 0.031	1.595HisIle: 1.595 ± 0.047	1.026HisLys: 1.026 ± 0.031	2.607HisLeu: 2.607 ± 0.065	0.594HisMet: 0.594 ± 0.028	0.715HisAsn: 0.715 ± 0.032	1.511HisPro: 1.511 ± 0.042	1.003HisGln: 1.003 ± 0.038	1.259HisArg: 1.259 ± 0.041	1.3HisSer: 1.3 ± 0.037	1.144HisThr: 1.144 ± 0.038	1.876HisVal: 1.876 ± 0.055	0.254HisTrp: 0.254 ± 0.018	1.037HisTyr: 1.037 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.746IleAla: 5.746 ± 0.094	0.781IleCys: 0.781 ± 0.031	4.258IleAsp: 4.258 ± 0.08	4.063IleGlu: 4.063 ± 0.075	2.934IlePhe: 2.934 ± 0.07	5.578IleGly: 5.578 ± 0.093	1.919IleHis: 1.919 ± 0.046	4.886IleIle: 4.886 ± 0.085	3.648IleLys: 3.648 ± 0.071	6.766IleLeu: 6.766 ± 0.091	1.709IleMet: 1.709 ± 0.047	2.742IleAsn: 2.742 ± 0.067	3.243IlePro: 3.243 ± 0.066	2.486IleGln: 2.486 ± 0.058	4.583IleArg: 4.583 ± 0.071	4.804IleSer: 4.804 ± 0.088	3.753IleThr: 3.753 ± 0.067	4.833IleVal: 4.833 ± 0.074	0.678IleTrp: 0.678 ± 0.031	2.301IleTyr: 2.301 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	4.85LysAla: 4.85 ± 0.093	0.325LysCys: 0.325 ± 0.022	3.274LysAsp: 3.274 ± 0.073	4.604LysGlu: 4.604 ± 0.078	1.641LysPhe: 1.641 ± 0.043	4.07LysGly: 4.07 ± 0.075	1.245LysHis: 1.245 ± 0.042	4.46LysIle: 4.46 ± 0.058	6.008LysLys: 6.008 ± 0.09	5.18LysLeu: 5.18 ± 0.083	2.131LysMet: 2.131 ± 0.049	3.002LysAsn: 3.002 ± 0.071	2.165LysPro: 2.165 ± 0.049	2.595LysGln: 2.595 ± 0.06	3.561LysArg: 3.561 ± 0.076	3.376LysSer: 3.376 ± 0.066	3.587LysThr: 3.587 ± 0.07	4.042LysVal: 4.042 ± 0.076	0.724LysTrp: 0.724 ± 0.03	2.02LysTyr: 2.02 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	7.742LeuAla: 7.742 ± 0.108	0.826LeuCys: 0.826 ± 0.035	5.356LeuAsp: 5.356 ± 0.078	5.062LeuGlu: 5.062 ± 0.083	4.945LeuPhe: 4.945 ± 0.101	6.1LeuGly: 6.1 ± 0.09	2.268LeuHis: 2.268 ± 0.05	7.967LeuIle: 7.967 ± 0.135	6.604LeuLys: 6.604 ± 0.098	9.809LeuLeu: 9.809 ± 0.143	2.636LeuMet: 2.636 ± 0.06	4.313LeuAsn: 4.313 ± 0.076	4.335LeuPro: 4.335 ± 0.074	3.165LeuGln: 3.165 ± 0.063	4.694LeuArg: 4.694 ± 0.092	7.622LeuSer: 7.622 ± 0.103	6.009LeuThr: 6.009 ± 0.077	6.171LeuVal: 6.171 ± 0.088	0.791LeuTrp: 0.791 ± 0.03	2.976LeuTyr: 2.976 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	2.266MetAla: 2.266 ± 0.054	0.166MetCys: 0.166 ± 0.015	1.614MetAsp: 1.614 ± 0.046	1.53MetGlu: 1.53 ± 0.048	0.874MetPhe: 0.874 ± 0.03	1.799MetGly: 1.799 ± 0.049	0.53MetHis: 0.53 ± 0.024	2.411MetIle: 2.411 ± 0.06	2.17MetLys: 2.17 ± 0.053	2.452MetLeu: 2.452 ± 0.056	0.891MetMet: 0.891 ± 0.036	1.419MetAsn: 1.419 ± 0.04	1.117MetPro: 1.117 ± 0.033	0.895MetGln: 0.895 ± 0.028	1.395MetArg: 1.395 ± 0.039	1.632MetSer: 1.632 ± 0.044	1.883MetThr: 1.883 ± 0.048	1.672MetVal: 1.672 ± 0.047	0.167MetTrp: 0.167 ± 0.015	0.62MetTyr: 0.62 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.548AsnAla: 2.548 ± 0.055	0.254AsnCys: 0.254 ± 0.016	1.941AsnAsp: 1.941 ± 0.054	2.338AsnGlu: 2.338 ± 0.071	1.367AsnPhe: 1.367 ± 0.044	2.986AsnGly: 2.986 ± 0.061	1.219AsnHis: 1.219 ± 0.042	2.652AsnIle: 2.652 ± 0.059	2.346AsnLys: 2.346 ± 0.061	3.416AsnLeu: 3.416 ± 0.073	1.029AsnMet: 1.029 ± 0.033	1.409AsnAsn: 1.409 ± 0.052	2.021AsnPro: 2.021 ± 0.054	1.891AsnGln: 1.891 ± 0.053	2.472AsnArg: 2.472 ± 0.058	1.775AsnSer: 1.775 ± 0.053	1.781AsnThr: 1.781 ± 0.051	2.4AsnVal: 2.4 ± 0.054	0.446AsnTrp: 0.446 ± 0.022	1.377AsnTyr: 1.377 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	3.323ProAla: 3.323 ± 0.077	0.288ProCys: 0.288 ± 0.019	3.101ProAsp: 3.101 ± 0.071	3.539ProGlu: 3.539 ± 0.065	2.106ProPhe: 2.106 ± 0.05	3.178ProGly: 3.178 ± 0.064	0.821ProHis: 0.821 ± 0.032	2.242ProIle: 2.242 ± 0.055	1.989ProLys: 1.989 ± 0.06	3.845ProLeu: 3.845 ± 0.075	0.887ProMet: 0.887 ± 0.036	1.278ProAsn: 1.278 ± 0.042	1.147ProPro: 1.147 ± 0.044	1.188ProGln: 1.188 ± 0.042	1.411ProArg: 1.411 ± 0.04	2.302ProSer: 2.302 ± 0.057	1.611ProThr: 1.611 ± 0.044	3.889ProVal: 3.889 ± 0.073	0.418ProTrp: 0.418 ± 0.027	1.363ProTyr: 1.363 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	3.286GlnAla: 3.286 ± 0.069	0.203GlnCys: 0.203 ± 0.016	1.741GlnAsp: 1.741 ± 0.049	2.218GlnGlu: 2.218 ± 0.052	1.505GlnPhe: 1.505 ± 0.044	2.106GlnGly: 2.106 ± 0.05	0.745GlnHis: 0.745 ± 0.031	2.86GlnIle: 2.86 ± 0.06	2.763GlnLys: 2.763 ± 0.057	4.187GlnLeu: 4.187 ± 0.082	1.314GlnMet: 1.314 ± 0.038	1.573GlnAsn: 1.573 ± 0.05	1.445GlnPro: 1.445 ± 0.048	1.492GlnGln: 1.492 ± 0.046	1.675GlnArg: 1.675 ± 0.045	2.328GlnSer: 2.328 ± 0.054	2.141GlnThr: 2.141 ± 0.061	2.468GlnVal: 2.468 ± 0.053	0.419GlnTrp: 0.419 ± 0.023	1.145GlnTyr: 1.145 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	3.616ArgAla: 3.616 ± 0.065	0.4ArgCys: 0.4 ± 0.024	2.873ArgAsp: 2.873 ± 0.071	3.743ArgGlu: 3.743 ± 0.081	2.376ArgPhe: 2.376 ± 0.048	3.074ArgGly: 3.074 ± 0.066	1.381ArgHis: 1.381 ± 0.047	3.981ArgIle: 3.981 ± 0.072	3.981ArgLys: 3.981 ± 0.074	5.884ArgLeu: 5.884 ± 0.102	1.657ArgMet: 1.657 ± 0.047	2.112ArgAsn: 2.112 ± 0.063	2.119ArgPro: 2.119 ± 0.051	2.498ArgGln: 2.498 ± 0.063	3.662ArgArg: 3.662 ± 0.075	3.099ArgSer: 3.099 ± 0.06	2.909ArgThr: 2.909 ± 0.065	3.346ArgVal: 3.346 ± 0.07	0.506ArgTrp: 0.506 ± 0.027	1.865ArgTyr: 1.865 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	4.706SerAla: 4.706 ± 0.078	0.57SerCys: 0.57 ± 0.025	3.546SerAsp: 3.546 ± 0.073	3.825SerGlu: 3.825 ± 0.077	2.96SerPhe: 2.96 ± 0.065	5.952SerGly: 5.952 ± 0.094	1.397SerHis: 1.397 ± 0.046	4.203SerIle: 4.203 ± 0.074	2.962SerLys: 2.962 ± 0.078	6.198SerLeu: 6.198 ± 0.096	1.636SerMet: 1.636 ± 0.048	1.927SerAsn: 1.927 ± 0.05	2.279SerPro: 2.279 ± 0.057	1.981SerGln: 1.981 ± 0.045	3.446SerArg: 3.446 ± 0.062	3.858SerSer: 3.858 ± 0.097	2.73SerThr: 2.73 ± 0.062	4.474SerVal: 4.474 ± 0.084	0.714SerTrp: 0.714 ± 0.032	1.809SerTyr: 1.809 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	4.523ThrAla: 4.523 ± 0.079	0.503ThrCys: 0.503 ± 0.027	3.123ThrAsp: 3.123 ± 0.063	3.142ThrGlu: 3.142 ± 0.072	2.613ThrPhe: 2.613 ± 0.057	5.11ThrGly: 5.11 ± 0.086	1.151ThrHis: 1.151 ± 0.035	3.662ThrIle: 3.662 ± 0.076	2.541ThrLys: 2.541 ± 0.058	5.547ThrLeu: 5.547 ± 0.092	1.197ThrMet: 1.197 ± 0.044	1.684ThrAsn: 1.684 ± 0.043	2.314ThrPro: 2.314 ± 0.055	1.516ThrGln: 1.516 ± 0.04	2.651ThrArg: 2.651 ± 0.054	2.967ThrSer: 2.967 ± 0.055	2.534ThrThr: 2.534 ± 0.059	4.423ThrVal: 4.423 ± 0.075	0.532ThrTrp: 0.532 ± 0.026	1.746ThrTyr: 1.746 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	5.159ValAla: 5.159 ± 0.09	0.726ValCys: 0.726 ± 0.029	3.718ValAsp: 3.718 ± 0.069	3.425ValGlu: 3.425 ± 0.075	3.051ValPhe: 3.051 ± 0.061	4.089ValGly: 4.089 ± 0.078	1.612ValHis: 1.612 ± 0.048	5.468ValIle: 5.468 ± 0.078	3.848ValLys: 3.848 ± 0.081	7.004ValLeu: 7.004 ± 0.11	1.809ValMet: 1.809 ± 0.046	2.828ValAsn: 2.828 ± 0.064	3.04ValPro: 3.04 ± 0.065	2.332ValGln: 2.332 ± 0.048	3.863ValArg: 3.863 ± 0.078	4.79ValSer: 4.79 ± 0.081	4.321ValThr: 4.321 ± 0.077	4.669ValVal: 4.669 ± 0.08	0.626ValTrp: 0.626 ± 0.028	2.167ValTyr: 2.167 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.626TrpAla: 0.626 ± 0.026	0.073TrpCys: 0.073 ± 0.009	0.479TrpAsp: 0.479 ± 0.026	0.522TrpGlu: 0.522 ± 0.025	0.511TrpPhe: 0.511 ± 0.028	0.64TrpGly: 0.64 ± 0.029	0.254TrpHis: 0.254 ± 0.018	0.81TrpIle: 0.81 ± 0.034	0.665TrpLys: 0.665 ± 0.028	1.275TrpLeu: 1.275 ± 0.048	0.337TrpMet: 0.337 ± 0.021	0.476TrpAsn: 0.476 ± 0.024	0.348TrpPro: 0.348 ± 0.024	0.407TrpGln: 0.407 ± 0.021	0.528TrpArg: 0.528 ± 0.024	0.624TrpSer: 0.624 ± 0.03	0.547TrpThr: 0.547 ± 0.026	0.578TrpVal: 0.578 ± 0.025	0.127TrpTrp: 0.127 ± 0.013	0.336TrpTyr: 0.336 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.285TyrAla: 2.285 ± 0.049	0.299TyrCys: 0.299 ± 0.018	1.866TyrAsp: 1.866 ± 0.052	1.741TyrGlu: 1.741 ± 0.052	1.716TyrPhe: 1.716 ± 0.043	2.482TyrGly: 2.482 ± 0.058	0.971TyrHis: 0.971 ± 0.035	2.045TyrIle: 2.045 ± 0.054	1.588TyrLys: 1.588 ± 0.049	3.526TyrLeu: 3.526 ± 0.071	0.762TyrMet: 0.762 ± 0.029	1.169TyrAsn: 1.169 ± 0.04	1.504TyrPro: 1.504 ± 0.044	1.617TyrGln: 1.617 ± 0.053	2.014TyrArg: 2.014 ± 0.052	1.853TyrSer: 1.853 ± 0.051	1.709TyrThr: 1.709 ± 0.048	2.004TyrVal: 2.004 ± 0.042	0.378TyrTrp: 0.378 ± 0.02	1.293TyrTyr: 1.293 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2735 proteins (833227 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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