Chelativorans sp. (strain BNC1)
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4530 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q11MW0|Q11MW0_CHESB NnrS OS=Chelativorans sp. (strain BNC1) OX=266779 GN=Meso_4289 PE=4 SV=1
MM1 pKa = 7.03 SQEE4 pKa = 3.93 LSLAQNHH11 pKa = 5.76 AWNLARR17 pKa = 11.84 TLMTPVILFKK27 pKa = 10.75 VDD29 pKa = 2.71 EE30 pKa = 4.62 DD31 pKa = 4.55 AYY33 pKa = 10.86 GVLPADD39 pKa = 5.06 DD40 pKa = 5.6 LDD42 pKa = 5.33 DD43 pKa = 5.84 DD44 pKa = 4.72 EE45 pKa = 6.16 VNALYY50 pKa = 9.8 FYY52 pKa = 11.0 DD53 pKa = 5.54 PYY55 pKa = 11.21 TGGQPVHH62 pKa = 6.44
Molecular weight: 6.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.846
IPC_protein 3.77
Toseland 3.567
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.77
Sillero 3.897
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|Q11LH4|Q11LH4_CHESB FAD linked oxidase-like protein OS=Chelativorans sp. (strain BNC1) OX=266779 GN=Meso_0347 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.25 GGRR28 pKa = 11.84 GVIAARR34 pKa = 11.84 RR35 pKa = 11.84 NRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4530
0
4530
1453148
41
2950
320.8
34.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.952 ± 0.037
0.834 ± 0.011
5.379 ± 0.03
6.344 ± 0.036
3.808 ± 0.023
8.495 ± 0.048
2.0 ± 0.018
5.501 ± 0.025
3.234 ± 0.027
10.023 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.491 ± 0.018
2.71 ± 0.025
5.136 ± 0.026
3.121 ± 0.022
7.356 ± 0.041
5.648 ± 0.028
5.123 ± 0.033
7.289 ± 0.029
1.289 ± 0.015
2.267 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here