Nocardia farcinica (strain IFM 10152)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia; Nocardia farcinica

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5941 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5Z2B6|Q5Z2B6_NOCFA Phosphoribosylformylglycinamidine cyclo-ligase OS=Nocardia farcinica (strain IFM 10152) OX=247156 GN=purM PE=3 SV=1
MM1 pKa = 7.67AAYY4 pKa = 10.47LDD6 pKa = 4.57LLLHH10 pKa = 6.5PAASEE15 pKa = 4.16VAGTVLGLVRR25 pKa = 11.84EE26 pKa = 4.38VAHH29 pKa = 6.82DD30 pKa = 4.81LIAGTGDD37 pKa = 3.26WFTGSSTGDD46 pKa = 3.17AGLGSSTPDD55 pKa = 3.03GGVGSSTGDD64 pKa = 2.85WFTGSSYY71 pKa = 11.22PP72 pKa = 4.39

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5YMW6|Q5YMW6_NOCFA Uncharacterized protein OS=Nocardia farcinica (strain IFM 10152) OX=247156 GN=NFA_56230 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.75GRR42 pKa = 11.84ATLTAA47 pKa = 4.27

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5941

0

5941

1885749

32

14474

317.4

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.161 ± 0.05

0.751 ± 0.009

6.111 ± 0.036

5.565 ± 0.025

2.825 ± 0.019

8.921 ± 0.036

2.237 ± 0.016

3.731 ± 0.024

1.813 ± 0.024

10.143 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.831 ± 0.015

1.79 ± 0.017

6.203 ± 0.041

2.699 ± 0.02

8.22 ± 0.039

4.662 ± 0.028

6.145 ± 0.025

8.692 ± 0.038

1.47 ± 0.013

2.031 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski