Rodentibacter heylii
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1998 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V3K6K5|A0A1V3K6K5_9PAST Aminotransferase OS=Rodentibacter heylii OX=1906744 GN=BKG89_07455 PE=4 SV=1
MM1 pKa = 7.32 YY2 pKa = 9.89 IDD4 pKa = 3.75 TSEE7 pKa = 4.48 LCDD10 pKa = 3.39 IYY12 pKa = 11.16 ADD14 pKa = 3.4 QVDD17 pKa = 4.16 VVEE20 pKa = 5.55 PIFSSFGGLTYY31 pKa = 9.96 FYY33 pKa = 11.39 GKK35 pKa = 8.46 VTTVKK40 pKa = 10.34 CFEE43 pKa = 4.59 SNGLITEE50 pKa = 4.13 VLEE53 pKa = 4.28 EE54 pKa = 4.16 NGEE57 pKa = 4.11 GRR59 pKa = 11.84 VLVIDD64 pKa = 3.74 GGGAVRR70 pKa = 11.84 RR71 pKa = 11.84 ALIDD75 pKa = 3.65 AEE77 pKa = 4.31 LAQLAADD84 pKa = 4.38 NGWEE88 pKa = 4.36 GIIVYY93 pKa = 9.79 GAVRR97 pKa = 11.84 QIQQLEE103 pKa = 4.1 NIEE106 pKa = 4.18 IGIHH110 pKa = 5.7 ALAPVPVRR118 pKa = 11.84 ADD120 pKa = 3.34 DD121 pKa = 3.97 KK122 pKa = 11.82 DD123 pKa = 3.75 NGEE126 pKa = 4.09 SDD128 pKa = 3.64 VAVNFGGVTFFPEE141 pKa = 4.3 DD142 pKa = 3.43 YY143 pKa = 10.81 VYY145 pKa = 11.59 ADD147 pKa = 3.49 LTGIILSQEE156 pKa = 4.06 PLDD159 pKa = 5.19 LEE161 pKa = 5.35 DD162 pKa = 5.62 FEE164 pKa = 5.6 QEE166 pKa = 3.8
Molecular weight: 18.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.528
ProMoST 3.846
Dawson 3.706
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.643
Nozaki 3.821
DTASelect 3.999
Thurlkill 3.567
EMBOSS 3.63
Sillero 3.834
Patrickios 1.837
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A1V3K0Z1|A0A1V3K0Z1_9PAST Probable alpha-L-glutamate ligase OS=Rodentibacter heylii OX=1906744 GN=rimK PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.4 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1998
0
1998
647336
37
3037
324.0
36.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.759 ± 0.059
1.061 ± 0.019
4.722 ± 0.045
6.198 ± 0.05
4.531 ± 0.049
6.612 ± 0.057
2.032 ± 0.027
7.769 ± 0.049
6.15 ± 0.048
10.698 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.333 ± 0.03
5.02 ± 0.052
3.702 ± 0.033
4.92 ± 0.054
4.49 ± 0.045
6.13 ± 0.036
5.188 ± 0.04
6.203 ± 0.045
1.159 ± 0.022
3.323 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here