Campylobacter sp. RM16192
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1867 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G5R5D0|A0A6G5R5D0_9PROT SCP-like extracellular protein OS=Campylobacter sp. RM16192 OX=1660080 GN=CDOMC_1425 PE=4 SV=1
MM1 pKa = 7.28 SVKK4 pKa = 9.21 ITDD7 pKa = 3.16 ICISCGSCIDD17 pKa = 3.71 EE18 pKa = 4.91 CPVEE22 pKa = 5.18 AIVDD26 pKa = 3.85 DD27 pKa = 4.11 SDD29 pKa = 3.86 NPTGEE34 pKa = 4.78 DD35 pKa = 2.69 IYY37 pKa = 11.57 YY38 pKa = 10.43 VYY40 pKa = 11.1 ADD42 pKa = 3.78 KK43 pKa = 11.13 CVEE46 pKa = 4.3 CVGHH50 pKa = 6.61 NDD52 pKa = 3.28 EE53 pKa = 4.91 PACASACPTDD63 pKa = 4.43 GCIVWDD69 pKa = 4.18 MPYY72 pKa = 10.19 EE73 pKa = 4.38 GQPSRR78 pKa = 11.84 SEE80 pKa = 3.53 ISADD84 pKa = 3.2 MRR86 pKa = 11.84 KK87 pKa = 10.5 GEE89 pKa = 4.21 TAVIAA94 pKa = 4.29
Molecular weight: 10.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.893
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.872
Patrickios 0.006
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A6G5R3P7|A0A6G5R3P7_9PROT Uncharacterized protein OS=Campylobacter sp. RM16192 OX=1660080 GN=CDOMC_0881 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPHH8 pKa = 5.76 KK9 pKa = 7.92 TPKK12 pKa = 9.96 KK13 pKa = 7.46 RR14 pKa = 11.84 THH16 pKa = 6.5 GFRR19 pKa = 11.84 QRR21 pKa = 11.84 MKK23 pKa = 9.24 TKK25 pKa = 10.33 NGRR28 pKa = 11.84 KK29 pKa = 9.12 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.98 GRR39 pKa = 11.84 RR40 pKa = 11.84 ILAAA44 pKa = 4.16
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1867
0
1867
556605
37
2261
298.1
33.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.123 ± 0.058
1.073 ± 0.022
5.69 ± 0.047
6.935 ± 0.058
5.489 ± 0.053
6.17 ± 0.074
1.549 ± 0.027
8.862 ± 0.058
8.988 ± 0.068
9.457 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.478 ± 0.026
5.784 ± 0.05
2.798 ± 0.033
2.658 ± 0.031
3.648 ± 0.04
6.731 ± 0.048
4.373 ± 0.035
6.016 ± 0.056
0.668 ± 0.016
3.509 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here