Anaerococcus vaginalis ATCC 51170
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1764 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7HTY6|C7HTY6_9FIRM S-adenosylmethionine:tRNA ribosyltransferase-isomerase OS=Anaerococcus vaginalis ATCC 51170 OX=655811 GN=queA PE=3 SV=1
MM1 pKa = 7.28 SKK3 pKa = 10.49 KK4 pKa = 10.74 LEE6 pKa = 4.03 IHH8 pKa = 4.66 NHH10 pKa = 5.08 EE11 pKa = 4.38 IEE13 pKa = 4.06 FEE15 pKa = 4.2 KK16 pKa = 11.41 NEE18 pKa = 3.73 GKK20 pKa = 10.53 AIIEE24 pKa = 4.19 LQLDD28 pKa = 3.9 EE29 pKa = 6.12 NPIQCYY35 pKa = 10.28 LIDD38 pKa = 3.98 IFSVDD43 pKa = 3.38 GVDD46 pKa = 4.39 YY47 pKa = 10.77 IALVDD52 pKa = 4.1 SEE54 pKa = 4.21 NSEE57 pKa = 4.91 LIILLYY63 pKa = 10.33 EE64 pKa = 4.49 LEE66 pKa = 4.65 DD67 pKa = 3.96 EE68 pKa = 4.49 EE69 pKa = 5.44 SGEE72 pKa = 4.0 IKK74 pKa = 10.79 LNSLEE79 pKa = 5.7 DD80 pKa = 3.8 EE81 pKa = 4.92 DD82 pKa = 5.48 KK83 pKa = 11.28 LDD85 pKa = 4.44 EE86 pKa = 4.68 IYY88 pKa = 10.89 HH89 pKa = 6.22 LFSHH93 pKa = 6.13 YY94 pKa = 9.71 WDD96 pKa = 4.16 YY97 pKa = 11.28 DD98 pKa = 3.9 TIDD101 pKa = 4.44 EE102 pKa = 4.51 IVSEE106 pKa = 4.43 YY107 pKa = 10.88 EE108 pKa = 3.93 SDD110 pKa = 5.67 LEE112 pKa = 5.56 DD113 pKa = 4.52 SDD115 pKa = 5.89 LNEE118 pKa = 4.01
Molecular weight: 13.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.77
IPC_protein 3.732
Toseland 3.541
ProMoST 3.846
Dawson 3.706
Bjellqvist 3.884
Wikipedia 3.592
Rodwell 3.567
Grimsley 3.452
Solomon 3.694
Lehninger 3.643
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.579
EMBOSS 3.617
Sillero 3.846
Patrickios 1.024
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|C7HWC1|C7HWC1_9FIRM Oligopeptide ABC transporter permease protein AppB OS=Anaerococcus vaginalis ATCC 51170 OX=655811 GN=appB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.21 QPNRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.94 RR12 pKa = 11.84 KK13 pKa = 8.48 KK14 pKa = 9.79 DD15 pKa = 2.78 HH16 pKa = 6.25 GFRR19 pKa = 11.84 KK20 pKa = 10.0 RR21 pKa = 11.84 MSTPAGRR28 pKa = 11.84 RR29 pKa = 11.84 VLKK32 pKa = 10.45 SRR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.59 GRR39 pKa = 11.84 KK40 pKa = 8.7 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.017
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1764
0
1764
562464
37
4748
318.9
36.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.234 ± 0.058
0.89 ± 0.024
6.584 ± 0.058
7.709 ± 0.067
4.84 ± 0.053
5.902 ± 0.068
1.191 ± 0.02
9.811 ± 0.079
10.534 ± 0.085
9.148 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.463 ± 0.028
6.562 ± 0.06
2.533 ± 0.035
2.327 ± 0.032
3.221 ± 0.042
6.393 ± 0.043
4.315 ± 0.046
5.589 ± 0.052
0.547 ± 0.017
4.205 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here