Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylococcus; Methylococcus capsulatus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2925 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q60B46|Q60B46_METCA Uncharacterized protein OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=MCA0636 PE=4 SV=1
MM1 pKa = 7.2TSEE4 pKa = 4.69LGIFYY9 pKa = 10.57RR10 pKa = 11.84SDD12 pKa = 4.01FDD14 pKa = 5.31RR15 pKa = 11.84AWCDD19 pKa = 3.29MSAEE23 pKa = 4.43TIKK26 pKa = 10.38FTDD29 pKa = 3.17SAAQRR34 pKa = 11.84VSEE37 pKa = 4.15LLAEE41 pKa = 4.81EE42 pKa = 4.99GDD44 pKa = 3.75QNLKK48 pKa = 10.45LRR50 pKa = 11.84VYY52 pKa = 8.88VTGGGCSGFQYY63 pKa = 10.9GFTFDD68 pKa = 3.8EE69 pKa = 4.88VVNEE73 pKa = 4.34DD74 pKa = 3.56DD75 pKa = 4.42TVVEE79 pKa = 4.27KK80 pKa = 11.32NGVSVLVDD88 pKa = 3.57PMSLQYY94 pKa = 10.95LQGAEE99 pKa = 3.42IDD101 pKa = 3.75YY102 pKa = 9.89TEE104 pKa = 4.52NVSGSQFVIRR114 pKa = 11.84NPNATTTCGCGSSFSVV130 pKa = 3.54

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q60BR7|Q60BR7_METCA Putative oxidoreductase Gfo/Idh/MocA family OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=MCA0398 PE=4 SV=1
MM1 pKa = 7.64HH2 pKa = 7.41GPGLPARR9 pKa = 11.84AGRR12 pKa = 11.84RR13 pKa = 11.84LQRR16 pKa = 11.84SGLGRR21 pKa = 11.84MGRR24 pKa = 11.84RR25 pKa = 11.84SGQPHH30 pKa = 7.31PGAVLPGGTLGRR42 pKa = 11.84RR43 pKa = 11.84LQSGAGRR50 pKa = 11.84EE51 pKa = 3.97RR52 pKa = 11.84HH53 pKa = 6.02RR54 pKa = 11.84AQQPAGRR61 pKa = 11.84AHH63 pKa = 6.53LQQRR67 pKa = 11.84GIRR70 pKa = 11.84LQGTLHH76 pKa = 7.26RR77 pKa = 11.84LRR79 pKa = 11.84PRR81 pKa = 11.84LRR83 pKa = 11.84LLRR86 pKa = 11.84RR87 pKa = 11.84HH88 pKa = 5.89LRR90 pKa = 11.84RR91 pKa = 11.84HH92 pKa = 4.47VRR94 pKa = 11.84TTVRR98 pKa = 11.84QTVIPSGTGSKK109 pKa = 9.88PAVAASAGPSKK120 pKa = 10.9AGASRR125 pKa = 11.84DD126 pKa = 3.41APVRR130 pKa = 11.84QRR132 pKa = 11.84GDD134 pKa = 2.83

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2925

0

2925

971767

24

3349

332.2

36.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.185 ± 0.054

1.097 ± 0.017

5.406 ± 0.033

6.304 ± 0.048

3.792 ± 0.028

8.509 ± 0.046

2.315 ± 0.021

4.768 ± 0.031

3.207 ± 0.033

10.9 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.022

2.595 ± 0.024

5.388 ± 0.037

3.219 ± 0.026

7.845 ± 0.049

5.245 ± 0.032

4.808 ± 0.035

7.347 ± 0.034

1.393 ± 0.02

2.519 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski