Amino acid dipepetide frequency for Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.213AlaAla: 14.213 ± 0.17	1.276AlaCys: 1.276 ± 0.038	6.002AlaAsp: 6.002 ± 0.083	7.818AlaGlu: 7.818 ± 0.12	3.819AlaPhe: 3.819 ± 0.064	10.477AlaGly: 10.477 ± 0.124	2.179AlaHis: 2.179 ± 0.052	5.163AlaIle: 5.163 ± 0.077	3.332AlaLys: 3.332 ± 0.058	12.625AlaLeu: 12.625 ± 0.163	2.838AlaMet: 2.838 ± 0.062	2.574AlaAsn: 2.574 ± 0.057	4.827AlaPro: 4.827 ± 0.088	3.428AlaGln: 3.428 ± 0.068	8.325AlaArg: 8.325 ± 0.106	5.499AlaSer: 5.499 ± 0.076	4.705AlaThr: 4.705 ± 0.073	8.643AlaVal: 8.643 ± 0.115	1.604AlaTrp: 1.604 ± 0.042	2.5AlaTyr: 2.5 ± 0.06	0.0AlaXaa: 0.0 ± 0.0
Cys	1.126CysAla: 1.126 ± 0.037	0.16CysCys: 0.16 ± 0.013	0.606CysAsp: 0.606 ± 0.023	0.602CysGlu: 0.602 ± 0.024	0.378CysPhe: 0.378 ± 0.021	1.12CysGly: 1.12 ± 0.036	0.487CysHis: 0.487 ± 0.039	0.475CysIle: 0.475 ± 0.023	0.254CysLys: 0.254 ± 0.018	1.108CysLeu: 1.108 ± 0.034	0.194CysMet: 0.194 ± 0.017	0.295CysAsn: 0.295 ± 0.018	0.63CysPro: 0.63 ± 0.026	0.3CysGln: 0.3 ± 0.019	1.015CysArg: 1.015 ± 0.032	0.641CysSer: 0.641 ± 0.029	0.493CysThr: 0.493 ± 0.026	0.673CysVal: 0.673 ± 0.031	0.129CysTrp: 0.129 ± 0.011	0.284CysTyr: 0.284 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	5.983AspAla: 5.983 ± 0.089	0.656AspCys: 0.656 ± 0.028	2.81AspAsp: 2.81 ± 0.052	3.456AspGlu: 3.456 ± 0.066	2.298AspPhe: 2.298 ± 0.048	4.701AspGly: 4.701 ± 0.082	1.189AspHis: 1.189 ± 0.034	2.705AspIle: 2.705 ± 0.054	1.644AspLys: 1.644 ± 0.051	5.676AspLeu: 5.676 ± 0.095	1.084AspMet: 1.084 ± 0.031	1.183AspAsn: 1.183 ± 0.037	3.42AspPro: 3.42 ± 0.06	1.552AspGln: 1.552 ± 0.039	4.356AspArg: 4.356 ± 0.062	2.356AspSer: 2.356 ± 0.05	2.505AspThr: 2.505 ± 0.056	3.766AspVal: 3.766 ± 0.064	0.995AspTrp: 0.995 ± 0.033	1.729AspTyr: 1.729 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	7.44GluAla: 7.44 ± 0.135	0.558GluCys: 0.558 ± 0.025	2.897GluAsp: 2.897 ± 0.054	3.675GluGlu: 3.675 ± 0.083	2.303GluPhe: 2.303 ± 0.051	4.47GluGly: 4.47 ± 0.066	1.517GluHis: 1.517 ± 0.041	3.667GluIle: 3.667 ± 0.065	2.385GluLys: 2.385 ± 0.067	6.563GluLeu: 6.563 ± 0.091	1.356GluMet: 1.356 ± 0.043	1.77GluAsn: 1.77 ± 0.045	2.974GluPro: 2.974 ± 0.056	2.362GluGln: 2.362 ± 0.057	5.838GluArg: 5.838 ± 0.107	3.19GluSer: 3.19 ± 0.064	3.587GluThr: 3.587 ± 0.072	4.349GluVal: 4.349 ± 0.073	0.759GluTrp: 0.759 ± 0.029	1.379GluTyr: 1.379 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	3.885PheAla: 3.885 ± 0.074	0.413PheCys: 0.413 ± 0.023	2.557PheAsp: 2.557 ± 0.051	2.37PheGlu: 2.37 ± 0.048	1.579PhePhe: 1.579 ± 0.041	3.57PheGly: 3.57 ± 0.065	0.883PheHis: 0.883 ± 0.03	1.616PheIle: 1.616 ± 0.041	1.062PheLys: 1.062 ± 0.036	3.828PheLeu: 3.828 ± 0.075	0.741PheMet: 0.741 ± 0.029	1.124PheAsn: 1.124 ± 0.034	1.892PhePro: 1.892 ± 0.048	1.14PheGln: 1.14 ± 0.04	2.782PheArg: 2.782 ± 0.06	2.282PheSer: 2.282 ± 0.051	1.825PheThr: 1.825 ± 0.044	2.807PheVal: 2.807 ± 0.055	0.567PheTrp: 0.567 ± 0.027	1.002PheTyr: 1.002 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	8.023GlyAla: 8.023 ± 0.117	1.197GlyCys: 1.197 ± 0.035	4.275GlyAsp: 4.275 ± 0.079	5.321GlyGlu: 5.321 ± 0.072	3.801GlyPhe: 3.801 ± 0.075	7.248GlyGly: 7.248 ± 0.137	1.977GlyHis: 1.977 ± 0.046	4.747GlyIle: 4.747 ± 0.081	3.397GlyLys: 3.397 ± 0.065	9.052GlyLeu: 9.052 ± 0.112	2.233GlyMet: 2.233 ± 0.053	2.237GlyAsn: 2.237 ± 0.063	3.073GlyPro: 3.073 ± 0.059	2.838GlyGln: 2.838 ± 0.064	6.711GlyArg: 6.711 ± 0.096	4.675GlySer: 4.675 ± 0.085	4.195GlyThr: 4.195 ± 0.063	6.195GlyVal: 6.195 ± 0.083	1.405GlyTrp: 1.405 ± 0.045	2.489GlyTyr: 2.489 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.509HisAla: 2.509 ± 0.052	0.31HisCys: 0.31 ± 0.019	1.186HisAsp: 1.186 ± 0.036	1.194HisGlu: 1.194 ± 0.034	0.969HisPhe: 0.969 ± 0.032	2.241HisGly: 2.241 ± 0.059	0.698HisHis: 0.698 ± 0.031	1.007HisIle: 1.007 ± 0.03	0.563HisLys: 0.563 ± 0.024	2.37HisLeu: 2.37 ± 0.056	0.471HisMet: 0.471 ± 0.023	0.496HisAsn: 0.496 ± 0.027	1.735HisPro: 1.735 ± 0.051	0.654HisGln: 0.654 ± 0.027	1.944HisArg: 1.944 ± 0.055	1.068HisSer: 1.068 ± 0.033	1.054HisThr: 1.054 ± 0.034	1.586HisVal: 1.586 ± 0.042	0.41HisTrp: 0.41 ± 0.02	0.682HisTyr: 0.682 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.89IleAla: 5.89 ± 0.087	0.455IleCys: 0.455 ± 0.019	3.159IleAsp: 3.159 ± 0.06	3.592IleGlu: 3.592 ± 0.067	1.449IlePhe: 1.449 ± 0.04	4.372IleGly: 4.372 ± 0.081	1.206IleHis: 1.206 ± 0.039	1.769IleIle: 1.769 ± 0.049	1.424IleLys: 1.424 ± 0.041	4.483IleLeu: 4.483 ± 0.068	0.804IleMet: 0.804 ± 0.033	1.256IleAsn: 1.256 ± 0.042	2.681IlePro: 2.681 ± 0.055	1.415IleGln: 1.415 ± 0.043	3.728IleArg: 3.728 ± 0.066	2.366IleSer: 2.366 ± 0.056	2.302IleThr: 2.302 ± 0.05	3.697IleVal: 3.697 ± 0.072	0.483IleTrp: 0.483 ± 0.023	1.152IleTyr: 1.152 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	3.752LysAla: 3.752 ± 0.073	0.219LysCys: 0.219 ± 0.016	1.738LysAsp: 1.738 ± 0.045	1.861LysGlu: 1.861 ± 0.051	0.884LysPhe: 0.884 ± 0.03	2.537LysGly: 2.537 ± 0.064	0.671LysHis: 0.671 ± 0.03	1.588LysIle: 1.588 ± 0.039	1.37LysLys: 1.37 ± 0.047	3.31LysLeu: 3.31 ± 0.071	0.643LysMet: 0.643 ± 0.025	0.972LysAsn: 0.972 ± 0.039	1.977LysPro: 1.977 ± 0.048	1.083LysGln: 1.083 ± 0.036	2.348LysArg: 2.348 ± 0.051	1.827LysSer: 1.827 ± 0.048	1.953LysThr: 1.953 ± 0.049	2.28LysVal: 2.28 ± 0.056	0.327LysTrp: 0.327 ± 0.018	0.735LysTyr: 0.735 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	13.464LeuAla: 13.464 ± 0.168	1.128LeuCys: 1.128 ± 0.039	6.284LeuAsp: 6.284 ± 0.084	7.169LeuGlu: 7.169 ± 0.106	3.922LeuPhe: 3.922 ± 0.075	8.984LeuGly: 8.984 ± 0.128	2.305LeuHis: 2.305 ± 0.048	4.954LeuIle: 4.954 ± 0.081	3.88LeuLys: 3.88 ± 0.077	11.512LeuLeu: 11.512 ± 0.166	2.186LeuMet: 2.186 ± 0.056	2.847LeuAsn: 2.847 ± 0.055	6.178LeuPro: 6.178 ± 0.082	3.258LeuGln: 3.258 ± 0.059	8.033LeuArg: 8.033 ± 0.103	6.261LeuSer: 6.261 ± 0.082	5.268LeuThr: 5.268 ± 0.073	7.552LeuVal: 7.552 ± 0.106	1.384LeuTrp: 1.384 ± 0.042	2.428LeuTyr: 2.428 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.624MetAla: 2.624 ± 0.048	0.137MetCys: 0.137 ± 0.01	1.035MetAsp: 1.035 ± 0.031	1.278MetGlu: 1.278 ± 0.041	0.663MetPhe: 0.663 ± 0.026	1.58MetGly: 1.58 ± 0.044	0.472MetHis: 0.472 ± 0.023	1.079MetIle: 1.079 ± 0.035	0.963MetLys: 0.963 ± 0.03	2.307MetLeu: 2.307 ± 0.051	0.436MetMet: 0.436 ± 0.024	0.784MetAsn: 0.784 ± 0.026	1.303MetPro: 1.303 ± 0.038	0.748MetGln: 0.748 ± 0.029	1.568MetArg: 1.568 ± 0.04	1.312MetSer: 1.312 ± 0.041	1.298MetThr: 1.298 ± 0.035	1.454MetVal: 1.454 ± 0.04	0.162MetTrp: 0.162 ± 0.015	0.368MetTyr: 0.368 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.712AsnAla: 2.712 ± 0.053	0.296AsnCys: 0.296 ± 0.018	1.319AsnAsp: 1.319 ± 0.045	1.309AsnGlu: 1.309 ± 0.036	0.966AsnPhe: 0.966 ± 0.03	2.31AsnGly: 2.31 ± 0.059	0.622AsnHis: 0.622 ± 0.027	1.22AsnIle: 1.22 ± 0.043	0.69AsnLys: 0.69 ± 0.029	2.951AsnLeu: 2.951 ± 0.062	0.457AsnMet: 0.457 ± 0.022	0.644AsnAsn: 0.644 ± 0.03	2.049AsnPro: 2.049 ± 0.049	0.835AsnGln: 0.835 ± 0.031	2.087AsnArg: 2.087 ± 0.051	1.16AsnSer: 1.16 ± 0.045	1.259AsnThr: 1.259 ± 0.042	1.913AsnVal: 1.913 ± 0.05	0.392AsnTrp: 0.392 ± 0.022	0.76AsnTyr: 0.76 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	6.449ProAla: 6.449 ± 0.097	0.534ProCys: 0.534 ± 0.026	3.502ProAsp: 3.502 ± 0.055	4.42ProGlu: 4.42 ± 0.076	2.033ProPhe: 2.033 ± 0.044	4.985ProGly: 4.985 ± 0.083	1.158ProHis: 1.158 ± 0.037	1.905ProIle: 1.905 ± 0.046	1.453ProLys: 1.453 ± 0.038	5.396ProLeu: 5.396 ± 0.079	1.178ProMet: 1.178 ± 0.037	1.265ProAsn: 1.265 ± 0.037	3.104ProPro: 3.104 ± 0.071	1.599ProGln: 1.599 ± 0.039	3.26ProArg: 3.26 ± 0.067	2.754ProSer: 2.754 ± 0.064	2.263ProThr: 2.263 ± 0.06	4.479ProVal: 4.479 ± 0.068	0.785ProTrp: 0.785 ± 0.027	1.354ProTyr: 1.354 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.975GlnAla: 3.975 ± 0.068	0.33GlnCys: 0.33 ± 0.02	1.504GlnAsp: 1.504 ± 0.044	1.717GlnGlu: 1.717 ± 0.044	1.149GlnPhe: 1.149 ± 0.033	2.607GlnGly: 2.607 ± 0.062	0.694GlnHis: 0.694 ± 0.028	1.651GlnIle: 1.651 ± 0.042	1.119GlnLys: 1.119 ± 0.035	2.935GlnLeu: 2.935 ± 0.063	0.689GlnMet: 0.689 ± 0.031	0.881GlnAsn: 0.881 ± 0.035	1.822GlnPro: 1.822 ± 0.046	1.276GlnGln: 1.276 ± 0.043	2.777GlnArg: 2.777 ± 0.059	1.65GlnSer: 1.65 ± 0.038	1.825GlnThr: 1.825 ± 0.046	2.381GlnVal: 2.381 ± 0.056	0.463GlnTrp: 0.463 ± 0.02	0.747GlnTyr: 0.747 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	7.008ArgAla: 7.008 ± 0.092	0.901ArgCys: 0.901 ± 0.027	3.924ArgAsp: 3.924 ± 0.07	4.921ArgGlu: 4.921 ± 0.076	3.412ArgPhe: 3.412 ± 0.06	5.03ArgGly: 5.03 ± 0.075	2.232ArgHis: 2.232 ± 0.05	4.552ArgIle: 4.552 ± 0.07	2.541ArgLys: 2.541 ± 0.056	9.923ArgLeu: 9.923 ± 0.117	1.916ArgMet: 1.916 ± 0.042	2.155ArgAsn: 2.155 ± 0.048	4.033ArgPro: 4.033 ± 0.073	3.084ArgGln: 3.084 ± 0.062	6.956ArgArg: 6.956 ± 0.109	3.927ArgSer: 3.927 ± 0.066	3.396ArgThr: 3.396 ± 0.064	5.033ArgVal: 5.033 ± 0.087	1.215ArgTrp: 1.215 ± 0.034	2.291ArgTyr: 2.291 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	5.804SerAla: 5.804 ± 0.086	0.599SerCys: 0.599 ± 0.025	2.676SerAsp: 2.676 ± 0.06	2.901SerGlu: 2.901 ± 0.062	2.063SerPhe: 2.063 ± 0.049	5.271SerGly: 5.271 ± 0.079	1.252SerHis: 1.252 ± 0.037	2.374SerIle: 2.374 ± 0.055	1.407SerLys: 1.407 ± 0.035	5.905SerLeu: 5.905 ± 0.079	1.155SerMet: 1.155 ± 0.036	1.227SerAsn: 1.227 ± 0.045	3.121SerPro: 3.121 ± 0.069	1.526SerGln: 1.526 ± 0.044	4.208SerArg: 4.208 ± 0.065	2.828SerSer: 2.828 ± 0.067	2.34SerThr: 2.34 ± 0.054	3.891SerVal: 3.891 ± 0.066	0.704SerTrp: 0.704 ± 0.028	1.197SerTyr: 1.197 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	5.52ThrAla: 5.52 ± 0.077	0.454ThrCys: 0.454 ± 0.023	2.457ThrAsp: 2.457 ± 0.048	2.693ThrGlu: 2.693 ± 0.051	1.586ThrPhe: 1.586 ± 0.047	4.962ThrGly: 4.962 ± 0.081	1.112ThrHis: 1.112 ± 0.036	2.021ThrIle: 2.021 ± 0.043	1.005ThrLys: 1.005 ± 0.035	6.059ThrLeu: 6.059 ± 0.082	0.817ThrMet: 0.817 ± 0.03	1.034ThrAsn: 1.034 ± 0.04	3.234ThrPro: 3.234 ± 0.068	1.386ThrGln: 1.386 ± 0.039	3.274ThrArg: 3.274 ± 0.059	2.241ThrSer: 2.241 ± 0.052	2.207ThrThr: 2.207 ± 0.059	4.338ThrVal: 4.338 ± 0.067	0.601ThrTrp: 0.601 ± 0.027	1.075ThrTyr: 1.075 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	7.789ValAla: 7.789 ± 0.101	0.872ValCys: 0.872 ± 0.03	4.007ValAsp: 4.007 ± 0.064	4.768ValGlu: 4.768 ± 0.072	2.989ValPhe: 2.989 ± 0.065	5.394ValGly: 5.394 ± 0.088	1.567ValHis: 1.567 ± 0.045	3.658ValIle: 3.658 ± 0.073	2.405ValLys: 2.405 ± 0.061	7.993ValLeu: 7.993 ± 0.103	1.683ValMet: 1.683 ± 0.045	2.066ValAsn: 2.066 ± 0.057	3.905ValPro: 3.905 ± 0.066	2.195ValGln: 2.195 ± 0.05	5.529ValArg: 5.529 ± 0.081	4.305ValSer: 4.305 ± 0.075	3.789ValThr: 3.789 ± 0.063	5.77ValVal: 5.77 ± 0.096	0.933ValTrp: 0.933 ± 0.029	1.855ValTyr: 1.855 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	1.142TrpAla: 1.142 ± 0.031	0.158TrpCys: 0.158 ± 0.013	0.665TrpAsp: 0.665 ± 0.029	0.693TrpGlu: 0.693 ± 0.026	0.574TrpPhe: 0.574 ± 0.027	0.929TrpGly: 0.929 ± 0.035	0.36TrpHis: 0.36 ± 0.018	0.695TrpIle: 0.695 ± 0.025	0.508TrpLys: 0.508 ± 0.024	1.927TrpLeu: 1.927 ± 0.054	0.308TrpMet: 0.308 ± 0.018	0.53TrpAsn: 0.53 ± 0.026	0.657TrpPro: 0.657 ± 0.026	0.557TrpGln: 0.557 ± 0.025	1.25TrpArg: 1.25 ± 0.04	0.785TrpSer: 0.785 ± 0.03	0.676TrpThr: 0.676 ± 0.031	0.923TrpVal: 0.923 ± 0.031	0.208TrpTrp: 0.208 ± 0.018	0.389TrpTyr: 0.389 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.541TyrAla: 2.541 ± 0.054	0.318TyrCys: 0.318 ± 0.019	1.457TyrAsp: 1.457 ± 0.04	1.282TyrGlu: 1.282 ± 0.037	1.086TyrPhe: 1.086 ± 0.037	2.302TyrGly: 2.302 ± 0.053	0.568TyrHis: 0.568 ± 0.027	0.838TyrIle: 0.838 ± 0.033	0.672TyrLys: 0.672 ± 0.027	3.072TyrLeu: 3.072 ± 0.064	0.382TyrMet: 0.382 ± 0.019	0.61TyrAsn: 0.61 ± 0.027	1.262TyrPro: 1.262 ± 0.032	0.957TyrGln: 0.957 ± 0.031	2.459TyrArg: 2.459 ± 0.054	1.321TyrSer: 1.321 ± 0.043	1.141TyrThr: 1.141 ± 0.039	1.74TyrVal: 1.74 ± 0.045	0.409TyrTrp: 0.409 ± 0.024	0.776TyrTyr: 0.776 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2925 proteins (971767 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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