Vibrio phage vB_VorS-PVo5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Ermolyevavirinae; unclassified Ermolyevavirinae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9BA64|A0A0N9BA64_9CAUD Uncharacterized protein OS=Vibrio phage vB_VorS-PVo5 OX=1689660 GN=AEO54_221 PE=4 SV=1
MM1 pKa = 7.42QINSTTIGATEE12 pKa = 4.02GAINIYY18 pKa = 10.62LDD20 pKa = 3.45GEE22 pKa = 4.08EE23 pKa = 4.65AYY25 pKa = 10.43GVVNLHH31 pKa = 5.42TQGKK35 pKa = 8.65YY36 pKa = 8.68IEE38 pKa = 5.35RR39 pKa = 11.84YY40 pKa = 8.78VKK42 pKa = 10.38DD43 pKa = 4.01DD44 pKa = 3.54NGHH47 pKa = 5.55FVIDD51 pKa = 3.81GDD53 pKa = 4.2EE54 pKa = 4.29LMLEE58 pKa = 4.06CVPFNTATVVFDD70 pKa = 3.59NVVIEE75 pKa = 4.45VII77 pKa = 3.74

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9BA43|A0A0N9BA43_9CAUD Uncharacterized protein OS=Vibrio phage vB_VorS-PVo5 OX=1689660 GN=AEO54_133 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.39KK3 pKa = 9.75RR4 pKa = 11.84GKK6 pKa = 6.6MQKK9 pKa = 9.51IAMFALAYY17 pKa = 10.19GSSIPLACEE26 pKa = 3.46KK27 pKa = 10.83SGLGLRR33 pKa = 11.84RR34 pKa = 11.84TKK36 pKa = 10.16TLAKK40 pKa = 10.05HH41 pKa = 4.93IAEE44 pKa = 3.99NGFRR48 pKa = 11.84IRR50 pKa = 11.84NN51 pKa = 3.61

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

23758

37

1594

255.5

28.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.039 ± 0.666

0.947 ± 0.124

6.402 ± 0.222

6.886 ± 0.341

3.712 ± 0.232

6.638 ± 0.272

1.89 ± 0.124

5.872 ± 0.256

7.471 ± 0.199

8.279 ± 0.438

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.142

5.114 ± 0.192

3.628 ± 0.219

3.67 ± 0.135

4.504 ± 0.208

6.6 ± 0.429

6.406 ± 0.277

6.28 ± 0.206

1.33 ± 0.107

3.914 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski