Sewage-associated circular DNA virus-7

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075J468|A0A075J468_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-7 OX=1519396 PE=4 SV=1
MM1 pKa = 7.68ARR3 pKa = 11.84RR4 pKa = 11.84QGIYY8 pKa = 9.05WLLTIPHH15 pKa = 6.26SEE17 pKa = 4.05FTPYY21 pKa = 10.64LPPPVAWIRR30 pKa = 11.84GQLEE34 pKa = 4.24LGAGGYY40 pKa = 7.29LHH42 pKa = 6.69WQAVLAFRR50 pKa = 11.84SKK52 pKa = 11.21GSLATVQAIFGTAMHH67 pKa = 6.82AEE69 pKa = 4.42ISRR72 pKa = 11.84SSAASDD78 pKa = 3.91YY79 pKa = 10.57VWKK82 pKa = 10.46EE83 pKa = 3.8DD84 pKa = 3.46TRR86 pKa = 11.84VSGTQFEE93 pKa = 4.53LGEE96 pKa = 4.27RR97 pKa = 11.84PFKK100 pKa = 11.15RR101 pKa = 11.84NDD103 pKa = 3.05PRR105 pKa = 11.84DD106 pKa = 3.12WDD108 pKa = 5.17AIWEE112 pKa = 4.2MAVAGNLCGIPASVRR127 pKa = 11.84VQSYY131 pKa = 7.02RR132 pKa = 11.84TLRR135 pKa = 11.84AICADD140 pKa = 3.05HH141 pKa = 6.49SKK143 pKa = 10.23PVGMEE148 pKa = 3.66RR149 pKa = 11.84TCFVFWGRR157 pKa = 11.84TGTGKK162 pKa = 9.99SRR164 pKa = 11.84RR165 pKa = 11.84AWDD168 pKa = 3.43EE169 pKa = 4.06AGVDD173 pKa = 4.36AYY175 pKa = 11.56SKK177 pKa = 11.13DD178 pKa = 3.53PNTKK182 pKa = 9.54FWCGYY187 pKa = 7.95NGQRR191 pKa = 11.84NVVIDD196 pKa = 3.81EE197 pKa = 3.85FRR199 pKa = 11.84GRR201 pKa = 11.84IDD203 pKa = 3.04VSHH206 pKa = 7.15FLRR209 pKa = 11.84WLDD212 pKa = 3.54RR213 pKa = 11.84YY214 pKa = 9.27PVNVEE219 pKa = 3.75IKK221 pKa = 10.51GSSTPLCCEE230 pKa = 3.95RR231 pKa = 11.84VWITSNLDD239 pKa = 2.94PRR241 pKa = 11.84AWYY244 pKa = 10.44SEE246 pKa = 3.88LDD248 pKa = 3.84EE249 pKa = 4.47EE250 pKa = 4.85TLNALLRR257 pKa = 11.84RR258 pKa = 11.84LNITHH263 pKa = 7.31FLL265 pKa = 3.74

Molecular weight: 30.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075J468|A0A075J468_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-7 OX=1519396 PE=4 SV=1
MM1 pKa = 7.39IKK3 pKa = 10.07KK4 pKa = 9.51HH5 pKa = 5.81FKK7 pKa = 10.27SIKK10 pKa = 9.78KK11 pKa = 10.14GIAAAATAASLRR23 pKa = 11.84RR24 pKa = 11.84SNQQRR29 pKa = 11.84LAQRR33 pKa = 11.84KK34 pKa = 8.09RR35 pKa = 11.84VGRR38 pKa = 11.84SRR40 pKa = 11.84TKK42 pKa = 10.38TKK44 pKa = 10.28TKK46 pKa = 8.93TKK48 pKa = 10.04RR49 pKa = 11.84FRR51 pKa = 11.84TITDD55 pKa = 3.17DD56 pKa = 3.68SGIGSSAHH64 pKa = 5.74LVVVDD69 pKa = 4.23AKK71 pKa = 10.71KK72 pKa = 10.8KK73 pKa = 9.04KK74 pKa = 6.44TAKK77 pKa = 10.33GKK79 pKa = 8.89WIYY82 pKa = 8.8TQTHH86 pKa = 5.72NEE88 pKa = 4.29VIFSNAGTQGTQQLGMGALSQINVSSGVGYY118 pKa = 8.88TGFQSQVAVRR128 pKa = 11.84QLNPMLGNTGSAYY141 pKa = 9.3LTASTAPTTDD151 pKa = 2.84SFVISDD157 pKa = 4.18MIFDD161 pKa = 4.51IEE163 pKa = 4.22MCSWSDD169 pKa = 3.4VAQTVDD175 pKa = 3.17MYY177 pKa = 11.63VYY179 pKa = 10.11VAKK182 pKa = 10.61DD183 pKa = 3.32GQANDD188 pKa = 4.33VGIEE192 pKa = 3.85WARR195 pKa = 11.84GYY197 pKa = 8.37QQQGSGKK204 pKa = 10.26GAMTMLSAPLYY215 pKa = 10.1QGVAGYY221 pKa = 10.96GRR223 pKa = 11.84VDD225 pKa = 3.56EE226 pKa = 5.49PYY228 pKa = 11.03ASPLDD233 pKa = 3.61VKK235 pKa = 10.92EE236 pKa = 4.12YY237 pKa = 10.62MNNTYY242 pKa = 10.84RR243 pKa = 11.84FMKK246 pKa = 10.25KK247 pKa = 7.39VTINLTSGATHH258 pKa = 6.72KK259 pKa = 10.96CKK261 pKa = 10.47VHH263 pKa = 6.72CVMNKK268 pKa = 9.69LVNNSEE274 pKa = 4.33LDD276 pKa = 3.27VDD278 pKa = 4.14TRR280 pKa = 11.84GSDD283 pKa = 3.48LTIKK287 pKa = 10.71GLTTIIMITVRR298 pKa = 11.84GQVVDD303 pKa = 3.59DD304 pKa = 3.94TTAGGTAKK312 pKa = 9.21PTFASTRR319 pKa = 11.84VGLITRR325 pKa = 11.84NTYY328 pKa = 9.22VCHH331 pKa = 6.27PVPSPISNRR340 pKa = 11.84TVGIHH345 pKa = 6.43SYY347 pKa = 10.44NVPINAALAAQQLINDD363 pKa = 3.98VDD365 pKa = 4.29VVAQAINCC373 pKa = 4.04

Molecular weight: 40.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

898

260

373

299.3

32.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.572 ± 1.0

1.559 ± 0.251

4.009 ± 0.921

4.12 ± 1.257

2.784 ± 0.503

7.795 ± 0.259

1.782 ± 0.215

5.122 ± 0.544

5.011 ± 1.034

7.35 ± 1.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.561 ± 0.419

3.898 ± 0.603

5.679 ± 1.794

4.009 ± 0.827

6.459 ± 1.136

9.688 ± 1.959

8.018 ± 1.069

7.238 ± 0.945

2.45 ± 0.932

2.895 ± 0.443
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski