Panagrellus redivivus (Microworm)
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26326 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7E4VBK4|A0A7E4VBK4_PANRE Uncharacterized protein OS=Panagrellus redivivus OX=6233 PE=4 SV=1
MM1 pKa = 7.62 SNRR4 pKa = 11.84 SIKK7 pKa = 9.23 TLNEE11 pKa = 3.65 AEE13 pKa = 4.62 EE14 pKa = 4.17 VHH16 pKa = 6.23 SLYY19 pKa = 10.91 VFQDD23 pKa = 3.05 TDD25 pKa = 3.96 DD26 pKa = 4.76 DD27 pKa = 4.29 VASVYY32 pKa = 11.13 AFDD35 pKa = 5.09 APSKK39 pKa = 10.19 ICFKK43 pKa = 10.7 GSSSAYY49 pKa = 10.03 AFWSTLFFSVWAYY62 pKa = 9.19 LQYY65 pKa = 11.06 VIAYY69 pKa = 8.87 LYY71 pKa = 10.8 QIDD74 pKa = 4.14 NNSIIAEE81 pKa = 4.37 TVNSNLNSEE90 pKa = 4.1 VDD92 pKa = 3.35 TLKK95 pKa = 11.1 ANKK98 pKa = 9.99 ADD100 pKa = 4.19 DD101 pKa = 4.72 DD102 pKa = 4.59 SVSVYY107 pKa = 11.07 AFDD110 pKa = 5.29 NSDD113 pKa = 3.8 SPSTDD118 pKa = 2.99 ADD120 pKa = 3.8 KK121 pKa = 10.5 DD122 pKa = 3.72 TDD124 pKa = 3.99 DD125 pKa = 4.15 VEE127 pKa = 4.54 SVYY130 pKa = 11.27 AFDD133 pKa = 5.37 GADD136 pKa = 3.24 NDD138 pKa = 4.82 EE139 pKa = 4.6 IQSSDD144 pKa = 3.51 DD145 pKa = 3.62 TEE147 pKa = 5.19 GSVSSEE153 pKa = 3.84 SSNSFGFTIPNLGEE167 pKa = 4.13 GEE169 pKa = 4.25 QYY171 pKa = 10.97 GYY173 pKa = 10.6 HH174 pKa = 6.97 ADD176 pKa = 3.66 SDD178 pKa = 4.09 DD179 pKa = 3.84 EE180 pKa = 5.95 RR181 pKa = 11.84 FDD183 pKa = 3.85 FYY185 pKa = 11.96 NNVTCVRR192 pKa = 11.84 LVTGEE197 pKa = 4.24 SLTLAFDD204 pKa = 3.89 GQNLKK209 pKa = 10.64 VVIHH213 pKa = 7.06 DD214 pKa = 4.02 GKK216 pKa = 10.96 AYY218 pKa = 9.7 TSFDD222 pKa = 3.49 AMPSDD227 pKa = 4.41 VADD230 pKa = 4.11 LLIAARR236 pKa = 11.84 ARR238 pKa = 11.84 IIDD241 pKa = 3.76 LVFF244 pKa = 4.55
Molecular weight: 26.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.516
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.427
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.139
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.872
Patrickios 1.214
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.777
Protein with the highest isoelectric point:
>tr|A0A7E4VW86|A0A7E4VW86_PANRE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit KCP2 OS=Panagrellus redivivus OX=6233 PE=3 SV=1
AA1 pKa = 7.85 RR2 pKa = 11.84 PLASNQPPARR12 pKa = 11.84 QPKK15 pKa = 9.37 ARR17 pKa = 11.84 HH18 pKa = 5.32 QGTVRR23 pKa = 11.84 RR24 pKa = 11.84 PRR26 pKa = 11.84 APLKK30 pKa = 10.56 ARR32 pKa = 11.84 PLASNQPPARR42 pKa = 11.84 QPKK45 pKa = 8.68 ARR47 pKa = 11.84 HH48 pKa = 5.99 RR49 pKa = 11.84 GTARR53 pKa = 11.84 RR54 pKa = 11.84 PKK56 pKa = 10.42 APLKK60 pKa = 10.51 ARR62 pKa = 11.84 PLASNQPPARR72 pKa = 11.84 QPKK75 pKa = 9.44 ARR77 pKa = 11.84 PLATNPPPARR87 pKa = 11.84 QPKK90 pKa = 9.41 ARR92 pKa = 11.84 PLASNQPPARR102 pKa = 11.84 QPKK105 pKa = 9.32 ARR107 pKa = 11.84 HH108 pKa = 5.95 RR109 pKa = 11.84 DD110 pKa = 3.16 SARR113 pKa = 11.84 RR114 pKa = 11.84 PRR116 pKa = 11.84 APLKK120 pKa = 10.56 ARR122 pKa = 11.84 PLASNQPPAA131 pKa = 4.28
Molecular weight: 14.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.691
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.214
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26288
38
26326
10071718
11
9598
382.6
42.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.581 ± 0.024
1.958 ± 0.015
5.584 ± 0.015
5.902 ± 0.024
4.453 ± 0.015
5.408 ± 0.02
2.399 ± 0.008
5.494 ± 0.015
5.712 ± 0.022
8.472 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.317 ± 0.008
4.668 ± 0.012
5.827 ± 0.02
3.772 ± 0.016
5.468 ± 0.017
7.734 ± 0.022
6.257 ± 0.021
6.646 ± 0.012
1.02 ± 0.005
3.138 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here