Mycobacterium virus Astro
Average proteome isoelectric point is 5.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6RA54|I6RA54_9CAUD Uncharacterized protein OS=Mycobacterium virus Astro OX=1195075 GN=98 PE=4 SV=1
MM1 pKa = 7.24 ARR3 pKa = 11.84 IYY5 pKa = 10.83 VADD8 pKa = 4.32 LAAYY12 pKa = 8.91 NAGVLHH18 pKa = 6.5 GEE20 pKa = 4.15 WIDD23 pKa = 3.77 LNGRR27 pKa = 11.84 DD28 pKa = 4.51 SEE30 pKa = 4.57 EE31 pKa = 3.44 VWAEE35 pKa = 4.1 VKK37 pKa = 10.92 AMLAEE42 pKa = 4.34 GQKK45 pKa = 10.79 LYY47 pKa = 11.0 VSKK50 pKa = 9.52 TLGVHH55 pKa = 5.61 EE56 pKa = 4.84 EE57 pKa = 4.16 WAIHH61 pKa = 6.54 DD62 pKa = 4.03 YY63 pKa = 11.45 EE64 pKa = 4.98 GFGSLRR70 pKa = 11.84 IGEE73 pKa = 4.47 YY74 pKa = 10.72 EE75 pKa = 6.09 DD76 pKa = 3.71 IDD78 pKa = 4.26 QLIDD82 pKa = 3.92 LADD85 pKa = 3.83 ALEE88 pKa = 4.11 KK89 pKa = 10.75 HH90 pKa = 6.09 GEE92 pKa = 4.04 PFEE95 pKa = 4.38 AWLSEE100 pKa = 3.95 IIGDD104 pKa = 3.3 IGYY107 pKa = 8.55 FASVSDD113 pKa = 5.21 AEE115 pKa = 4.47 DD116 pKa = 3.27 QFGDD120 pKa = 3.65 RR121 pKa = 11.84 FIGAVTKK128 pKa = 10.56 EE129 pKa = 4.04 DD130 pKa = 3.52 YY131 pKa = 10.86 AYY133 pKa = 10.52 DD134 pKa = 3.74 YY135 pKa = 11.85 AEE137 pKa = 5.11 DD138 pKa = 3.84 VLGLTGVALDD148 pKa = 3.63 YY149 pKa = 11.09 FDD151 pKa = 4.31 YY152 pKa = 11.37 EE153 pKa = 4.1 KK154 pKa = 10.54 FARR157 pKa = 11.84 DD158 pKa = 3.64 LEE160 pKa = 4.28 LSGDD164 pKa = 3.51 ISEE167 pKa = 5.13 CYY169 pKa = 10.48 YY170 pKa = 11.28 NGTDD174 pKa = 3.33 YY175 pKa = 11.44 LFRR178 pKa = 11.84 SWW180 pKa = 4.0
Molecular weight: 20.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.757
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.783
Grimsley 3.656
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.228
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.062
Patrickios 0.922
IPC_peptide 3.91
IPC2_peptide 4.05
IPC2.peptide.svr19 3.954
Protein with the highest isoelectric point:
>tr|I6RTV2|I6RTV2_9CAUD Uncharacterized protein OS=Mycobacterium virus Astro OX=1195075 GN=5 PE=4 SV=1
MM1 pKa = 7.4 SWLTSARR8 pKa = 11.84 RR9 pKa = 11.84 FDD11 pKa = 5.54 LPPDD15 pKa = 3.27 WEE17 pKa = 4.24 VRR19 pKa = 11.84 RR20 pKa = 11.84 QEE22 pKa = 4.38 VLDD25 pKa = 4.3 DD26 pKa = 4.24 ANGVCEE32 pKa = 4.31 IRR34 pKa = 11.84 GPGCLGWATEE44 pKa = 3.88 VDD46 pKa = 4.01 HH47 pKa = 7.09 IRR49 pKa = 11.84 RR50 pKa = 11.84 GNDD53 pKa = 2.65 HH54 pKa = 6.47 SRR56 pKa = 11.84 RR57 pKa = 11.84 NLQAACSKK65 pKa = 10.62 CHH67 pKa = 5.76 GKK69 pKa = 10.3 KK70 pKa = 10.37 SSAEE74 pKa = 3.6 GNARR78 pKa = 11.84 KK79 pKa = 9.28 RR80 pKa = 11.84 QLRR83 pKa = 11.84 AMRR86 pKa = 11.84 RR87 pKa = 11.84 RR88 pKa = 11.84 PPDD91 pKa = 3.2 RR92 pKa = 11.84 HH93 pKa = 6.14 PGSMM97 pKa = 3.91
Molecular weight: 11.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.18
IPC_protein 9.633
Toseland 10.613
ProMoST 10.248
Dawson 10.643
Bjellqvist 10.365
Wikipedia 10.833
Rodwell 10.745
Grimsley 10.657
Solomon 10.833
Lehninger 10.804
Nozaki 10.657
DTASelect 10.335
Thurlkill 10.599
EMBOSS 11.023
Sillero 10.613
Patrickios 10.57
IPC_peptide 10.847
IPC2_peptide 9.809
IPC2.peptide.svr19 8.409
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
99
0
99
16367
25
1025
165.3
18.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.33 ± 0.409
0.935 ± 0.134
6.984 ± 0.266
7.344 ± 0.283
3.379 ± 0.175
8.181 ± 0.328
1.912 ± 0.151
4.955 ± 0.205
4.662 ± 0.332
8.407 ± 0.271
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.536 ± 0.149
3.367 ± 0.181
5.151 ± 0.239
3.385 ± 0.208
5.78 ± 0.356
5.261 ± 0.192
6.171 ± 0.249
6.867 ± 0.204
2.261 ± 0.172
3.134 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here