Actinomyces graevenitzii C83

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; Actinomyces graevenitzii

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1852 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9PD70|G9PD70_9ACTO Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Actinomyces graevenitzii C83 OX=435830 GN=HMPREF0045_00349 PE=3 SV=1
MM1 pKa = 7.14ATDD4 pKa = 3.4YY5 pKa = 10.3DD6 pKa = 3.71APRR9 pKa = 11.84RR10 pKa = 11.84NEE12 pKa = 4.38DD13 pKa = 3.32EE14 pKa = 4.68PEE16 pKa = 4.33ADD18 pKa = 3.58SLEE21 pKa = 4.13EE22 pKa = 4.0LTTHH26 pKa = 6.75QKK28 pKa = 10.33DD29 pKa = 3.49QSSAAIEE36 pKa = 3.85EE37 pKa = 4.51DD38 pKa = 3.46EE39 pKa = 4.56NEE41 pKa = 3.9IAEE44 pKa = 4.35GFEE47 pKa = 4.77LPGADD52 pKa = 4.28LSRR55 pKa = 11.84EE56 pKa = 4.08EE57 pKa = 4.7LSVHH61 pKa = 5.87VVPQLEE67 pKa = 4.28DD68 pKa = 3.18EE69 pKa = 4.82FTCSEE74 pKa = 4.53CFLVHH79 pKa = 6.55HH80 pKa = 7.18RR81 pKa = 11.84SQLAYY86 pKa = 10.71VDD88 pKa = 4.63DD89 pKa = 4.18ATNLPVCTDD98 pKa = 3.56CAGG101 pKa = 3.17

Molecular weight: 11.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9PGJ6|G9PGJ6_9ACTO 30S ribosomal protein S6 OS=Actinomyces graevenitzii C83 OX=435830 GN=rpsF PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.56VHH17 pKa = 5.47GFRR20 pKa = 11.84HH21 pKa = 5.67RR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AVLAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.68GRR40 pKa = 11.84ARR42 pKa = 11.84LAAA45 pKa = 4.44

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1852

0

1852

644062

32

3141

347.8

37.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.521 ± 0.074

0.831 ± 0.016

5.444 ± 0.047

5.382 ± 0.059

2.857 ± 0.037

8.218 ± 0.051

1.979 ± 0.025

4.544 ± 0.039

3.537 ± 0.046

9.983 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.047 ± 0.025

3.011 ± 0.03

5.036 ± 0.047

4.089 ± 0.052

6.078 ± 0.06

6.504 ± 0.055

5.845 ± 0.053

8.194 ± 0.052

1.42 ± 0.023

2.478 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski