Agrococcus jejuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agrococcus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8A2I9|A0A1G8A2I9_9MICO FtsX-like permease family protein OS=Agrococcus jejuensis OX=399736 GN=SAMN04489720_0223 PE=4 SV=1
MM1 pKa = 8.19RR2 pKa = 11.84IRR4 pKa = 11.84PAVLVLAASVSLVGCTASDD23 pKa = 3.45PAPRR27 pKa = 11.84VTADD31 pKa = 3.51DD32 pKa = 3.89VADD35 pKa = 4.33RR36 pKa = 11.84WAQASQGAIDD46 pKa = 3.98YY47 pKa = 10.09QLGGAYY53 pKa = 10.06DD54 pKa = 4.06PAPGTTIVARR64 pKa = 11.84DD65 pKa = 3.58ASDD68 pKa = 3.92PPAEE72 pKa = 4.26GAFSICYY79 pKa = 10.32LNGFQTQPADD89 pKa = 3.84GEE91 pKa = 4.33WWLEE95 pKa = 3.82EE96 pKa = 4.27HH97 pKa = 7.31PDD99 pKa = 4.04LVLRR103 pKa = 11.84DD104 pKa = 3.81AAGEE108 pKa = 3.93PAIDD112 pKa = 4.38PGWPDD117 pKa = 3.67EE118 pKa = 4.2MALDD122 pKa = 4.07TSTPEE127 pKa = 3.62QRR129 pKa = 11.84EE130 pKa = 3.85QIAAIVTPWIAGCASDD146 pKa = 5.88GYY148 pKa = 11.27DD149 pKa = 3.73AVEE152 pKa = 4.44FDD154 pKa = 5.87NLDD157 pKa = 2.81SWTRR161 pKa = 11.84FDD163 pKa = 3.82GLTMEE168 pKa = 5.26GNMALAALLVDD179 pKa = 4.28AAHH182 pKa = 7.9DD183 pKa = 3.84EE184 pKa = 4.56GLWAAQKK191 pKa = 10.4NALDD195 pKa = 4.25AGEE198 pKa = 4.83AGPGAGFDD206 pKa = 3.49LVVTEE211 pKa = 4.41EE212 pKa = 4.01CGQYY216 pKa = 9.96EE217 pKa = 4.24EE218 pKa = 4.59CAAYY222 pKa = 10.35AEE224 pKa = 4.9LYY226 pKa = 10.33GDD228 pKa = 3.31RR229 pKa = 11.84HH230 pKa = 6.71VDD232 pKa = 3.01IEE234 pKa = 4.57YY235 pKa = 10.9VEE237 pKa = 4.46STSEE241 pKa = 3.85DD242 pKa = 3.55DD243 pKa = 3.54FAAMCEE249 pKa = 4.25AGDD252 pKa = 3.98VPPLTVLRR260 pKa = 11.84DD261 pKa = 3.46RR262 pKa = 11.84PLSVEE267 pKa = 3.86GDD269 pKa = 3.75DD270 pKa = 3.54GHH272 pKa = 7.94VRR274 pKa = 11.84VPCSS278 pKa = 2.98

Molecular weight: 29.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8EVG8|A0A1G8EVG8_9MICO T5orf172 domain-containing protein OS=Agrococcus jejuensis OX=399736 GN=SAMN04489720_2205 PE=4 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NRR31 pKa = 11.84KK32 pKa = 8.65

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3218

0

3218

1039962

29

5825

323.2

34.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.682 ± 0.082

0.497 ± 0.01

6.632 ± 0.042

5.54 ± 0.051

2.714 ± 0.026

9.104 ± 0.054

2.067 ± 0.029

4.202 ± 0.03

1.366 ± 0.031

10.0 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.85 ± 0.023

1.432 ± 0.026

5.485 ± 0.049

2.967 ± 0.034

7.339 ± 0.071

5.097 ± 0.031

6.119 ± 0.086

9.753 ± 0.049

1.486 ± 0.021

1.667 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski